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Enzymatic pretreatment and microwave extraction of asiaticoside from Centella asiatica
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Journal of Biomedical Science and Engineering·Volume 02 (2009)·Pages 526–531·Published 19 November 2009·DOI10.4236/jbise.2009.27076
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Abstract
The extraction of asiaticoside from Centella asiatica by enzymatic pretreatment and microwave extraction (EPME) was studied in this article. The effects of several important factors such as temperature of enzymatic pretreatment, liquid to solid ratio and microwave radiation time were investigated by quadric regression orthogonal design experiment and were analyzed by response surface. An extraction model with well forecast performance was then established. The results indicate that the optimum extraction condition was as follows: liquid to solid ratio was 36mL/g, temperature of enzymatic pretreatment was 45℃, enzymatic time was 30min, and microwave ra-diation time was 11
KeywordsAsiaticosideEnzymolysisMicrowave ExtractionCentella Asiatica
- Randriamampionona, D., Diallo, B., and Rakotoniriana, F., (2007) Comparative analysis of active constituents in Centella asiatica samples from Madagascar: Application for ex situ conservation and clonal propagation, Fitotera- pia, 78, 482–489.
- Verma, K., Bhartariya, G., and Gupta, M., (1999) Reversephase high performance liquid chromatography of asiaticoside in Centella asiatica, Phytochem. Anal., 10, 191–193.
- Sarma, K., Khosa, L., and Chansauria, N., (1995) Antistress activity of Tinospora cordfolia and Centella asiatica extracts, Phytother. Res., 10, 181?183.
- Flora, S. and Gupta, R., (2007) Beneficial effects of Centella asiatica aqueous extract against arsenic-induced oxidative stress and essential metal status in rats, Phytother. Res., 21, 980?988.
- Li, B. B., Smith, B., and Hossain, M., (2006) Extraction of phenolics from citrus peels II, Enzyme-assisted extraction method, Sep. Purif. Technol., 48, 189?196.
- Bayramoglu, B., Sahin, S., and Sumnu, G., (2008) Solventfree microwave extraction of essential oil from oregano, J. Food Eng., 88, 535?540.
- Proestos, C. and Komaitis, M., (2008) Application of micro-wave-assisted extraction to the fast extraction of plant phenolic compounds, Food Sci. Technol-LEB, 41, 652?659.
- Pan, X. J., Niu, G. G., and Liu, H. Z., (2003) Micro-wave-assisted extraction of tea polyphenols and tea caffeine from green tea leaves, Chem. Eng. Process, 42, 129? 133.
- Lu, X. Y., “Determination of asiaticosides by UV- spectropho-tography. (2005) J Modern Food Drug, 16, 15?16.
- Ma, S. F., Wang, L. Q., and Hu, Z. C., (2005) Enzymatic ex-traction of the submerged mycelium polysaccharide from Pleurotus nebrodensis, T CSAE, 22, 198?201.
- Virot, M., Tomao, V., and Colnagui, G., (2007) New micro-wave-integrated Soxhlet extraction an advantageous tool for the extraction of lipids from food products, J. Chromatogr. A, 1174, 138?144.