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Gold Nanoparticles/Thermochromic Composite Film on Screen-Printed Electrodes for Simultaneous Detection of Protein and Temperature
Biomedical Engineering Program, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana
School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, China
School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, China
School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, China
School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, China
- 1 Biomedical Engineering Program, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana
- 2 School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, China
- 3 School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, China
- 4 School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, China
- 5 School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, China
Journal of Biomaterials and Nanobiotechnology·Volume 12 (2021)·Pages 7–19·Published 22 April 2021·DOI10.4236/jbnb.2021.122002
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Abstract
In this study, gold nanoparticles and thermochromic composite films modified screen-printed carbon electrodes (TM-AuNPsSPCEs) were developed as a platform for the simultaneous detection of protein and temperature. The TM-AuNPs composited film had better sensitivity resulting from the gold nanoparticles amplification effect. A phase transition model analysis of TM-AuNPs films found that the TM-AuNPs films had three-phase transition intervals (<45℃, 45℃ to 80℃ and >80℃) which accommodated the temperature requirements for protein denaturation. When used to detect different concentrations of haemoglobin (Hb) solution, the TM-AuNPs modified SPCEs had a better sensitivity in detecting the different concentrations in comparison to TM and AuNP modified SPCEs which showed no clear sensitivity towards the different Hb concentrations. The dual detection and excellent sensitivity show a good application prospect for the study of the TM-AuNPs composite film.
KeywordsScreen-Printed Carbon ElectrodesGold NanoparticlesThermochromic MaterialSimultaneous Detection of Proteins and Temperature
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