Fabrication and Characterization of Chitosan Based Injectable Thermosensitive Hydrogels Containing Silica/Calcium Phosphate Nanocomposite Particles — Oak Academic Publishing
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Fabrication and Characterization of Chitosan Based Injectable Thermosensitive Hydrogels Containing Silica/Calcium Phosphate Nanocomposite Particles
Bone Engineering Science and Technology (BEST) Lab, Department of Biomedical Engineering, Virginia Commonwealth University, Richmond, VA, USA
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Department of Chemical and Life Sciences Engineering, Virginia Commonwealth University, Richmond, VA, USA
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Bone Engineering Science and Technology (BEST) Lab, Department of Biomedical Engineering, Virginia Commonwealth University, Richmond, VA, USA
1 Bone Engineering Science and Technology (BEST) Lab, Department of Biomedical Engineering, Virginia Commonwealth University, Richmond, VA, USA
2 Department of Chemical and Life Sciences Engineering, Virginia Commonwealth University, Richmond, VA, USA
3 Bone Engineering Science and Technology (BEST) Lab, Department of Biomedical Engineering, Virginia Commonwealth University, Richmond, VA, USA
In this study, the effect of silica/calcium phosphate (SiCaP) nanocomposite particles on the properties of a novel chitosan-based thermosensitive hydrogel system was examined. SiCaP nanocomposite powder was fabricated using a sol-gel method and then used to fabricate nanocomposite hydrogels (Ch- β /7.5SiCaP and Ch- β /15SiCaP) including chitosan and β -glycerophosphate (Ch- β ) as a matrix. Results revealed that compared to the Ch- β hydrogel without SiCaP, the presence of SiCaP particles in nanocomposite hydrogels maintained pH stability during the sol-gel transition, accelerated the gelation and improved the stiffness of nanocomposite hydrogels. Gelation time at 37℃ was reduced approximately 75% and stiffness was increased approximately 115%. Both of these changes are attributed to chemical and physical interactions of the SiCaP bioactive particles with chitosan. Furthermore, compared to the Ch- β hydrogel, the presence of SiCaP in the Ch- β /7.5SiCaP nanocomposite hydrogel did not affect biocompatibility negatively, but improved osteoblastic cell differentiation. Our studies suggest that these nanocomposite hydrogels may offer an innovative approach to bone regeneration strategies.
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