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Influence of Ozonized Oil Nanoemulsions on B-16 Melanoma Cells: An <i>in Vitro Study</i>
Department of Nanotechnology Engineering, Zonguldak Bülent Ecevit University, Zonguldak, Turkey
Department of Immunology, Facultyof Medicine, ZonguldakBülent Ecevit University, Zonguldak, Turkey
Department of Biomedical Engineering, Zonguldak Bülent Ecevit University, Zonguldak, Turkey
- 1 Department of Nanotechnology Engineering, Zonguldak Bülent Ecevit University, Zonguldak, Turkey
- 2 Department of Immunology, Facultyof Medicine, ZonguldakBülent Ecevit University, Zonguldak, Turkey
- 3 Department of Biomedical Engineering, Zonguldak Bülent Ecevit University, Zonguldak, Turkey
Journal of Biomaterials and Nanobiotechnology·Volume 12 (2021)·Pages 49–56·Published 17 August 2021·DOI10.4236/jbnb.2021.124005
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Abstract
In this study, we aimed to use a novel approach to overcome the current limitations of ozone therapy in medicine through ozonized oil nanoemulsions (OZNEs). We evaluated dose-dependency on the cellular activities of B-16 melanoma cell line which were incubated with various OZNE doses (v/v). Antitumor effects of OZNE against cancer cell lines were evaluated by cellular morphology, apoptosis and cell cycle analysis. Flow cytometry results showed that OZNE induced DNA damage, apoptosis, and arrested cell cycle in G0-1 phase in B-16 melanoma cells. Thus, OZNE treatment could pose an effective way to act as a potential therapeutic for patients with tumors in the future.
KeywordsOzonized OilNanoemulsionB-16 MelanomaCancer
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