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Green Synthesis and Characterization of Silver Nanoparticles Using Vachellia seyal Extract: A Comparative Physicochemical Study with Ascorbic Acid and Sodium Borohydride
Physical Pharmaceutics Laboratory, Faculty of Medicine, Pharmacy, and Odontology, Cheikh Anta Diop University, Dakar, Senegal
Galenic Pharmacy Laboratory, Faculty of Medicine, Pharmacy, and Odontology, Cheikh Anta Diop University, Dakar, Senegal
Physical Pharmaceutics Laboratory, Faculty of Medicine, Pharmacy, and Odontology, Cheikh Anta Diop University, Dakar, Senegal
Physical Pharmaceutics Laboratory, Faculty of Medicine, Pharmacy, and Odontology, Cheikh Anta Diop University, Dakar, Senegal
Physical Pharmaceutics Laboratory, Faculty of Medicine, Pharmacy, and Odontology, Cheikh Anta Diop University, Dakar, Senegal
Galenic Pharmacy Laboratory, Faculty of Medicine, Pharmacy, and Odontology, Cheikh Anta Diop University, Dakar, Senegal
Physical Pharmaceutics Laboratory, Faculty of Medicine, Pharmacy, and Odontology, Cheikh Anta Diop University, Dakar, Senegal
Physical Pharmaceutics Laboratory, Faculty of Medicine, Pharmacy, and Odontology, Cheikh Anta Diop University, Dakar, Senegal
Physical Pharmaceutics Laboratory, Faculty of Medicine, Pharmacy, and Odontology, Cheikh Anta Diop University, Dakar, Senegal
Physical Pharmaceutics Laboratory, Faculty of Medicine, Pharmacy, and Odontology, Cheikh Anta Diop University, Dakar, Senegal
Physical Pharmaceutics Laboratory, Faculty of Medicine, Pharmacy, and Odontology, Cheikh Anta Diop University, Dakar, Senegal
- 1 Physical Pharmaceutics Laboratory, Faculty of Medicine, Pharmacy, and Odontology, Cheikh Anta Diop University, Dakar, Senegal
- 2 Galenic Pharmacy Laboratory, Faculty of Medicine, Pharmacy, and Odontology, Cheikh Anta Diop University, Dakar, Senegal
- 3 Physical Pharmaceutics Laboratory, Faculty of Medicine, Pharmacy, and Odontology, Cheikh Anta Diop University, Dakar, Senegal
- 4 Physical Pharmaceutics Laboratory, Faculty of Medicine, Pharmacy, and Odontology, Cheikh Anta Diop University, Dakar, Senegal
- 5 Physical Pharmaceutics Laboratory, Faculty of Medicine, Pharmacy, and Odontology, Cheikh Anta Diop University, Dakar, Senegal
- 6 Galenic Pharmacy Laboratory, Faculty of Medicine, Pharmacy, and Odontology, Cheikh Anta Diop University, Dakar, Senegal
- 7 Physical Pharmaceutics Laboratory, Faculty of Medicine, Pharmacy, and Odontology, Cheikh Anta Diop University, Dakar, Senegal
- 8 Physical Pharmaceutics Laboratory, Faculty of Medicine, Pharmacy, and Odontology, Cheikh Anta Diop University, Dakar, Senegal
- 9 Physical Pharmaceutics Laboratory, Faculty of Medicine, Pharmacy, and Odontology, Cheikh Anta Diop University, Dakar, Senegal
- 10 Physical Pharmaceutics Laboratory, Faculty of Medicine, Pharmacy, and Odontology, Cheikh Anta Diop University, Dakar, Senegal
- 11 Physical Pharmaceutics Laboratory, Faculty of Medicine, Pharmacy, and Odontology, Cheikh Anta Diop University, Dakar, Senegal
Journal of Biomaterials and Nanobiotechnology·Volume 16 (2025)·Pages 21–30·Published 30 April 2025·DOI10.4236/jbnb.2025.162002
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Abstract
The green synthesis of silver nanoparticles (AgNPs) offers an eco-friendly and biocompatible alternative to conventional methods, which often involve toxic reagents. In this study, AgNPs were synthesized via chemical reduction using three different reducing agents: sodium borohydride (NaBH 4 ), ascorbic acid, and a Vachellia seyal plant extract. UV-Visible spectroscopy confirmed the formation of AgNPs, with characteristic surface plasmon resonance bands observed at 430 nm for all three reducing agents. Dynamic Light Scattering (DLS) analysis revealed average particle sizes of 18.69 ± 0.04 nm with a polydispersity index (PDI) of 0.639 for NaBH 4 , 42.44 ± 5.37 nm with a PDI of 0.234 ± 0.074 for ascorbic acid, and 129.53 ± 0.57 nm with a PDI of 0.092 ± 0.016 for V. seyal extract. Transmission Electron Microscopy (TEM) confirmed spherical morphology and sizes consistent with DLS measurements. These results indicate that the reducing strength of the agents significantly influences nanoparticle size and distribution. The V. seyal extract enabled the successful green synthesis of AgNPs with good size stability, highlighting its potential for pharmaceutical and biomedical applications.
KeywordsSilver NanoparticlesVachellia seyalGreen SynthesisDLSTEMPolydispersityAscorbic AcidNaBH 4
- Shahzadi, A. (2025) Plant-Mediated Green Synthesis and Biomedical Applications of Silver Nanoparticles: A Comparative Study Using Ocimum sanctum , Curcuma longa and Azadirachta indica Extracts.
- Haridas, E.S., Bhattacharya, S., Varma, M.K. and Chandra, G.K. (2022) Green Synthesis of Eco-Friendly Silver Nanoparticles Using Coffea arabica Leaf Extract and Development of a Biosensor for Cysteine. https://doi.org/10.48550/arXiv.2209.03823
- Do, J.-M., Hong, J.W. and Yoon, H.-S. (2025) Microalgae-Mediated Green Synthesis of Silver Nanoparticles: A Sustainable Approach Using Extracellular Polymeric Substances from Graesiella emersonii . Frontiers in Microbiology , 16, Article 1589285. https://doi.org/10.3389/fmicb.2025.1589285
- Khan, A.U., Hussain, T., Abdullah Khan, M.A., Almostafa, M.M., Younis, N.S. and Yahya, G. (2023) Antibacterial and Antibiofilm Activity of Ficus carica -Mediated Calcium Oxide (CaONPs) Phyto-Nanoparticles. Molecules , 28, Article 5553. http://doi.org/10.3390/molecules28145553
- Rajak, K.K., Pahilani, P.P., Patel, H., et al . (2023) Green Synthesis of Silver Nanoparticles Using Curcuma longa Flower Extract and Antibacterial Activity. https://doi.org/10.48550/arXiv.2304.04777
- Dilbar, S., Sher, H., Ali, A., et al . (2023) Antibacterial Efficacy of Green Synthesized Silver Nanoparticles Using Salvia nubicola Extract. ACS Omega , 8, 31155-31167. https://doi.org/10.1021/acsomega.3c03164
- Rizwana, H., Alzahrani, T., Alwahibi, M.S., et al . (2025) Phytofabrication of AgNPs Using Psidium guajava Leaf Extract: Antifungal Activity against Phytopathogens. BMC Plant Biology , 25, Article No. 65.
- Asefian, S. and Ghavam, M. (2024) Green and Environmentally Friendly Synthesis of Silver Nanoparticles with Antibacterial Properties from Some Medicinal Plants. BMC Biotechnology , 24, Article No. 5. https://doi.org/10.1186/s12896-023-00828-z
- Arshad, F., Naikoo, G.A., Hassan, I.U., Chava, S.R., El-Tanani, M., Aljabali, A.A., et al . (2024) Bioinspired and Green Synthesis of Silver Nanoparticles for Medical Applications: A Green Perspective. Applied Biochemistry and Biotechnology , 196, 3636-3669. https://doi.org/10.1007/s12010-023-04719-z
- Rehman, G., Umar, M., Shah, N., Hamayun, M., Ali, A., Khan, W., et al. (2023) Green Synthesis and Characterization of Silver Nanoparticles Using Azadirachta indica Seeds Extract: In Vitro and in Vivo Evaluation of Anti-Diabetic Activity. Pharmaceuticals , 16, Article 1677. https://doi.org/10.3390/ph16121677