Conjugation of Magnetite Nanoparticles with Melanogenesis Substrate, NPrCAP Provides Melanoma Targeted, <i>in Situ</i> Peptide Vaccine Immunotherapy through HSP Production by Chemo-Thermotherapy — Oak Academic Publishing
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Conjugation of Magnetite Nanoparticles with Melanogenesis Substrate, NPrCAP Provides Melanoma Targeted, <i>in Situ</i> Peptide Vaccine Immunotherapy through HSP Production by Chemo-Thermotherapy
Institute of Dermatology & Cutaneous Sciences, Sapporo, Japan
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Sapporo Medical University School of Medicine, Sapporo, Japan
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Department of Dermatology, Sapporo Medical University School of Medicine, Sapporo, Japan
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Department of Dermatology, Sapporo Medical University School of Medicine, Sapporo, Japan
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Department of Dermatology, Sapporo Medical University School of Medicine, Sapporo, Japan
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Department of Dermatology, Sapporo Medical University School of Medicine, Sapporo, Japan;
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Department of Chemical Engineering, Faculty of Engineering, Kyushu University, Fukuoka, Japan
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Department of Biotechnology, School of Engineering, Nagoya University, Nagoya, Japan
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Department of Chemistry, Fujita Health University School of Health Sciences, Toyoake, Japan
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Department of Chemistry, Fujita Health University School of Health Sciences, Toyoake, Japan
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Meito Sangyo Co., Nagoya, Japan
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Faculty of Health and Welfare, Kawasaki University of Medical Welfare, Kurashiki, Japan
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Department of Biological Chemistry, College of Bioscience and Biotechnology, Chubu University, Ka- sugai, Japan.
1 Institute of Dermatology & Cutaneous Sciences, Sapporo, Japan
2 Sapporo Medical University School of Medicine, Sapporo, Japan
3 Department of Dermatology, Sapporo Medical University School of Medicine, Sapporo, Japan
4 Department of Dermatology, Sapporo Medical University School of Medicine, Sapporo, Japan
5 Department of Dermatology, Sapporo Medical University School of Medicine, Sapporo, Japan
6 Department of Dermatology, Sapporo Medical University School of Medicine, Sapporo, Japan;
7 Department of Chemical Engineering, Faculty of Engineering, Kyushu University, Fukuoka, Japan
8 Department of Biotechnology, School of Engineering, Nagoya University, Nagoya, Japan
9 Department of Chemistry, Fujita Health University School of Health Sciences, Toyoake, Japan
10 Department of Chemistry, Fujita Health University School of Health Sciences, Toyoake, Japan
11 Meito Sangyo Co., Nagoya, Japan
12 Faculty of Health and Welfare, Kawasaki University of Medical Welfare, Kurashiki, Japan
13 Department of Biological Chemistry, College of Bioscience and Biotechnology, Chubu University, Ka- sugai, Japan.
In order to develop melanoma-targeted in situ peptide vaccine immunotherapy, magnetite nanoparticles were conjugated with a melanogenesis substrate, N-propionyl cysteaminylphenol (NPrCAP). Magnetite nanoparticles introduced thermotherapy which caused non-apoptotic cell death and generation of heat shock protein (HSP) upon exposure to alternating magnetic field (AMF). NPrCAP was expected to develop a melanoma-targeted therapeutic drug because of its selective incorporation into melanoma cells and production of highly reactive free radicals, that result in not only oxidative stress but also apoptotic cell death by reacting with tyrosinase.
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