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Multifunctional Biocompatible Fluorescent <i>Carboxymethyl Cellulose</i> Nanoparticles

¹ Bio Nano Electronics Research Center Graduate School of Interdisciplinary New Science Toyo University Kawagoe, Saitama, JAPAN
² Bio Nano Electronics Research Center Graduate School of Interdisciplinary New Science Toyo University Kawagoe, Saitama, JAPAN
³ Bio Nano Electronics Research Center Graduate School of Interdisciplinary New Science Toyo University Kawagoe, Saitama, JAPAN
⁴ Bio Nano Electronics Research Center Graduate School of Interdisciplinary New Science Toyo University Kawagoe, Saitama, JAPAN
⁵ Bio Nano Electronics Research Center Graduate School of Interdisciplinary New Science Toyo University Kawagoe, Saitama, JAPAN
⁶ Bio Nano Electronics Research Center Graduate School of Interdisciplinary New Science Toyo University Kawagoe, Saitama, JAPAN
⁷ Bio Nano Electronics Research Center Graduate School of Interdisciplinary New Science Toyo University Kawagoe, Saitama, JAPAN
⁸ Bio Nano Electronics Research Center Graduate School of Interdisciplinary New Science Toyo University Kawagoe, Saitama, JAPAN
⁹ Bio Nano Electronics Research Center Graduate School of Interdisciplinary New Science Toyo University Kawagoe, Saitama, JAPAN

Journal of Biomaterials and Nanobiotechnology·Volume 03 (2012)·Pages 254–261·Published 11 May 2012·DOI10.4236/jbnb.2012.322031

Abstract

A multifunctional nanoparticle based on carboxymethyl cellulose was developed. Folate group was attached to nanoparticle for specific recognition of cancerous cells and 5FU was encapsulated for delivering cytotoxicity. The whole system was able to track by the semiconductor quantum dots that were attached to the nanoparticle. The multifunctional nanoparticle was characterized by UV-VIS spectra, PL spectra, FTIR, TEM, SEM etc and was targeted to human breast cancer cell, MCF7. The biocompatibility of nanoparticle without drug and cytotoxicity rendered by nanoparticle with drug was studied with MCF7 and L929 cell lines. The epifluorescent images suggest that the folate-conjugated nanoparticles were more internalized by folate receptor positive cell line, MCF7 than the noncancerous L929 cells.

KeywordsMultifunctional&lti&gtCarboxymethyl Cellulose&lt/i&gtFolateNanoparticlesBiocompatible

D. E Reisner, “Bionanotechnology: Global Prospects,” CRC Press, Taylor & Francis Group, Boca Raton, 2009
V. P. Torchilin, “Multifunctional Nanocarriers,” Advanced Drug Delivery Reviews, Vol. 58, No. 14, 2006, pp. 1532-1555. doi:10.1016/j.addr.2006.09.009
N. Sanvicens and M. P. Marco, “Multifunctional Nanoparticles—Properties and Prospects for Their Use in Human Medicine,” Trends in Biotechnology, Vol. 26, No. 8, 2008, pp. 425-433. doi:10.1016/j.tibtech.2008.04.005
K. S. Soppimath, T. M. Aminabhavi, A. R Kulkarni and W. E. Rudzinski, “Biodegradable Polymeric Nanoparticles as Drug Delivery Devices,” Journal of Controlled Release, Vol. 70, No. 1-2, 2000, pp. 1-20. doi:10.1016/S0168-3659(00)00339-4
K.-T. Yong, I. Roy, M. T. Swihart and P. N. Prasad, “Multifunctional Nanoparticles as Biocompatible Targeted Probes for Hu-man Cancer Diagnosis and Therapy,” Journal of Materials Chemistry, Vol. 19, No. 27, 2009, pp. 4655-4672. doi:10.1039/b817667c
J. H. Gao, H. W. Gu and B. Xu, “Multifunctional Magnetic Nanoparticles: Design, Synthesis, and Biomedical Applications,” Accounts of Chemical Research, Vol. 42, No. 8, 2009, pp. 1097-1107. doi:10.1021/ar9000026
J. Kim, Y. Z. Piao and T. Hyeon, “Multifunctional Nanostructured Materials for Multimodal Imaging, and Simultaneous Imaging and Therapy,” Chemical Society Reviews, Vol. 38, No. 2, 2009, pp. 372-390. doi:10.1039/b709883a
D. R. Biswal and R. P. Singh, “Characterisation of Carboxymethyl cellulose and Polya-crylamide Graft Copolymer,” Carbohydrate Polymers, Vol. 57, No. 4, 2004, pp. 379-387. doi:10.1016/j.carbpol.2004.04.020
J. Pan and S. S. Feng, “Targeting and Imaging Cancer Cells by Folate Decorated Quantum Dots Loaded Nanoparticle of Bio-degradable Polymers,” Biomaterials, Vol. 30, No. 6, 2009, pp. 1176-1183. doi:10.1016/j.biomaterials.2008.10.039
M. E. Mathew, J. C. Mohan, K. Manzoor, S.V. Nair, H. Tamura and R. Jayakumar, “Folate Conjugated Carboxymethyl Chitosan-Manganese Doped Zinc Sulphide Nanoparticles for Targeted Drug Delivery and Imaging of Cancer Cells,” Carbohydrate Polymers, Vol. 80, No. 2, 2010, pp. 442-448. doi:10.1016/j.carbpol.2009.11.047
M. Brucher Jr., M. Moronne, P. Gin, S. Weiss and A. P. Alivisatos, “Semiconductor Nanocrystals as Fluorescent Biological Labels,” Science, Vol. 281, No. 5385, 1998, pp. 2013-2016. doi:10.1126/science.281.5385.2013
C. B Murray, D. J. Norris and M. G. Baewndi, “Synthesis and Characterization of Nearly Monodisperse CdE (E = Sulfur, Selenium, Tellurium) Semiconductor Nanocrystallites,” Journal of the American Chemical Society, Vol. 115, No. 19, 1993, pp. 8706-8715. doi:10.1021/ja00072a025

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Multifunctional Biocompatible Fluorescent <i>Carboxymethyl Cellulose</i> Nanoparticles

Abstract

References (17)