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Investigation of the Effect of Local Electrical Stimulation on Cells Cultured on Conductive Single-Walled Carbon Nanotube/Albumin Films
Center for Probe Microscopy and Nanotechnology, National Research University of Electronic Technology, Zelenograd, Moscow, Russia
Center for Probe Microscopy and Nanotechnology, National Research University of Electronic Technology, Zelenograd, Moscow, Russia
Center for Probe Microscopy and Nanotechnology, National Research University of Electronic Technology, Zelenograd, Moscow, Russia
Cell Culter Laboratory, Ivanovskiy Institute of Virology, Moscow, Russia.
Cell Culter Laboratory, Ivanovskiy Institute of Virology, Moscow, Russia.
Cell Culter Laboratory, Ivanovskiy Institute of Virology, Moscow, Russia.
- 1 Center for Probe Microscopy and Nanotechnology, National Research University of Electronic Technology, Zelenograd, Moscow, Russia
- 2 Center for Probe Microscopy and Nanotechnology, National Research University of Electronic Technology, Zelenograd, Moscow, Russia
- 3 Center for Probe Microscopy and Nanotechnology, National Research University of Electronic Technology, Zelenograd, Moscow, Russia
- 4 Cell Culter Laboratory, Ivanovskiy Institute of Virology, Moscow, Russia.
- 5 Cell Culter Laboratory, Ivanovskiy Institute of Virology, Moscow, Russia.
- 6 Cell Culter Laboratory, Ivanovskiy Institute of Virology, Moscow, Russia.
Journal of Biomaterials and Nanobiotechnology·Volume 03 (2012)·Pages 377–384·Published 3 July 2012·DOI10.4236/jbnb.2012.33036
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Abstract
In this study we have developed a biocompatible current-conductive coating based on carbon nanotubes and bovine serum albumin and have shown its efficiency in culturing cells in vitro. We investigate the proliferation of human embryonic fibroblast (HEF) cells, which were subjected to electrical stimulation when cultured on carbon nanotube surface. A weak increase in proliferation is demonstrated at stimulating field pulses up to 100 mV. It is assumed that the transport mechanism accompanied by higher synthesis of proteins and their polymerization may increase proliferative activity at low voltages. At higher voltages the motility and spatial organization of HEF cell is observed. As a result, a novel technique of supplying the cells with electric field through a system of micro- and nanosized electrodes and a biocompatible composite have been developed.
KeywordsConductive CompositeHuman Embryonic FibroblastsBovine Serum AlbuminElectrical StimulationProliferation
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