Cytotoxicity of Pristine C<sub>60</sub> Fullerene on Baby Hamster Kidney Cells in Solution
- 1 School of Public Health, Shandong University, Jinan, 250012, P. R. China
- 2 School of Public Health, Shandong University, Jinan, 250012, P. R. China
- 3 School of Public Health, Shandong University, Jinan, 250012, P. R. China
- 4 School of Public Health, Shandong University, Jinan, 250012, P. R. China
- 5 School of Public Health, Shandong University, Jinan, 250012, P. R. China
Abstract
Research on biological effects of C 60 and its derivatives is one of the hotspots in the area of biological effects of nanomaterials. Compared with surface-modified C 60 derivatives, reports on the biological effects of unmodified pristine C 60 are relatively less. This work aimed to investigate the interaction between baby hamster kidney cells and pristine C 60 in solution. The C 60 suspension was prepared using solvent exchange method and characterized by UV spectrophotometry, electronic transmission microscopy and dynamic light scattering techniques. The baby hamster kidney cells were incubated with different concentrations of C 60 suspensions, and light microscopy, cell counting kit 8 assay, and acridine orange staining were used to observe the cell growth and morphology. The results showed that C 60 could ihibit the cell growth and induce cell apoptosis with a dose-effect relationship. C 60 might enter cells and the possible way it enters cells were also proposed.
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