Study of Photoinduced Interaction between Calf Thymus-DNA and Bovine Serum Albumin Protein with H<sub>2</sub>Ti<sub>3</sub>O<sub>7</sub> Nanotubes — Oak Academic Publishing
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Study of Photoinduced Interaction between Calf Thymus-DNA and Bovine Serum Albumin Protein with H<sub>2</sub>Ti<sub>3</sub>O<sub>7</sub> Nanotubes
Department of Chemistry, Burdwan University, Burdwan, India
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Institute of Physics, Bhubaneswar, India
,
Durgapur Institute of Advanced technology and Management, Durgapur, India
,
Department of Chemistry, Burdwan University, Burdwan, India
1 Department of Chemistry, Burdwan University, Burdwan, India
2 Institute of Physics, Bhubaneswar, India
3 Durgapur Institute of Advanced technology and Management, Durgapur, India
4 Department of Chemistry, Burdwan University, Burdwan, India
Hydrogen titanate nanotubes were synthesized by hydrothermal process using 10 M NaOH and TiO 2 anatase powder. The material synthesized was characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM) and transmission electron microscopy (TEM) to ensure the structural and morphological characteristics. The interaction of calf thymus DNA (CT-DNA) and bovine serum albumin protein with suspended aqueous solution of titanate nanotubes was investigated using UV absorption spectroscopy and the apparent association constant was found to be, K b = 1.68 × 10 4 M -1 and K ap =5.41 × 10 3 M -1 for DNA and BSA respectively. Addition of the titanate nano material resulted quenching of fluorescence spectra of ethidium bromide-DNA in tris HCl buffer solution and that of aqueous protein solution. The apparent binding constant (K sv = 5.46 × 10 4 M -1 for DNA binding and K sv = 6.063 × 10 3 M -1 for protein binding) was deduced from relevant fluorescence quenching data using Stern-Volmer equation.
KeywordsPhotoinduced InteractionNanotubesHydrothermal Process
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