Effect of Nano - Titanium Dioxide with Different Antibiotics against Methicillin-Resistant Staphylococcus Aureus
- 1 Department of Materials Science, Gulbarga University, Gulbarga, Karnataka, India
- 2 Department of Physics, Gurmithkal, Yadgir, Karnataka, India
- 3 Materials Science Lab, S. S. Margol College, Shahabad, Gulbarga, Karnataka, India
- 4 Department of Materials Science, Gulbarga University, Gulbarga, Karnataka, India
Abstract
The different investigation has been carried out on the biological activities of titanium dioxide nanoparticle but the effect of this nano product on the antibacterial activity of different antibiotics has not been yet demonstrated. In this study the nano size TiO2 is synthesized using citric acid and alpha dextrose and the enhancement effect of TiO2 nanoparticle on the antibacterial activity of different antibiotics was evaluated against Methicillin-resistant Staphylococcus aureus (MRSA). During the present study, different concentrations of nano-scale TiO2 were tested to find out the best concentration that can have the most effective antibacterial property against the MRSA culture. Disk diffusion method was used to determine the antibacterial activity of these antibiotics in the absence and presence of sub inhibitory concentration of TiO2 nano particle. A clinical isolate of MRSA, isolated from Intensive Care Unit (ICU) was used as test strain. In the presence of sub-inhibitory concentration of TiO2 nanoparticle (20 µg/disc) the antibacterial activities of all antibiotics have been increased against test strain with minimum 2 mm to maximum 10mm. The highest increase in inhibitory zone for MRSA was observed against pencillin G and amikacin (each 10 mm). Conversely, in case of nalidixic acid, TiO2 nanoparticle showed a Synergic effect on the antibacterial activity of this antibiotic against test strain. These results signify that the TiO2 nanoparticle potentate the antimicrobial action of beta lactums, cephalosporins, aminoglycosides, glycopeptides, macrolids and lincosamides, tetracycline a possible utilization of nano compound in combination effect against MRSA.
- N. De San, O. Denis, M. F. Gasasira , R. De Mendonca , C. Nonhoff and M. J. Struelens, “Controlled evaluation Of The IDI-MRSA Assay for Detection of Colonization by Methicillin-Resistant, Staphylococcus aureus in Di- verse Mucocutaneous Specimens,” A.J Clin Microbiol, 2007, Vol. 45, No. 4, pp. 1098-1101.
- N. Safdar and D. G. Maki, “The Community of Risk Factors for Nosocomial Colonization and Infection with Anti-Microbial-Resistant Staphylococcus aureus, Entero- Coccus, Gramnegative Bacilli, Clostridium Difficile and Candida,” Ann Intern Med, Vol. 136, 2002, pp. 834-844.
- L. C. Braga, A. A. Leite, K. G. Xavier , J. A. Takahashi, M. P. Bemquerer, E. Chartone-Souza, et al. “Synergic interaction between pomegranate extract and antibiotics against Staphylococcus aureus,” Canadian Journal of Microbiology, Vol. 51, No. 7, 2005, pp. 541-547.
- G. C. Schito, “The Importance of the Development of Antibiotic Resistance in Staphylococcus aureus,” Clin Microbiol Infect, Vol. 12, Suppl. 1, 2006, pp. 3-8.
- S. Gibbons, “Phytochemicals for Bacterial Resistance. Strengths, Weaknesses and Opportunities,” Planta Med, Vol. 74, No. 6, 2008, pp. 594-602.
- G. D. Wright, “Resisting Resistance: New Chemical Strategies for Battling Superbugs,” Chemistry and Biology, Vol. 7, No. 6, 2000, pp. R127-R132.
- X. Wu, H. Liu, J. Liu, K. N. Haley, J. A. Treadway, J. P. Larson, et al., “Immunofluorescent Labeling of Cancer Marker Her2 and Other Cellular Targets with Semicon- ductor Quantum Dots,” Nat Biotechnol, Vol. 21, No. 1, 2003, pp. 41-46.
- J. D. Fortner, D. Y. Lyon, C. M. Sayes, A. M. Boyd, J. C. Falkner, E. M. Hotze, Alemany et al., “C-60 in Water: Nanocrystal Formation and Microbial Response,” Envi- ron Sci Technol, Vol. 39, No. 11, 2005, pp. 4307-4316.
- P. Li, J. Li, Q. Wu and J. Li, “Synergistic Antibacterial Effects of Lactum Antibiotic Combined with Silver Nanoparticles,” J. Nanotechnol, Vol. 16, No. 9, 2005, pp. 1912-1917.
- A. M. Smith, H. Duan, M. N. Rhyner, G. Ruan and S. A. Nie, “Synthesis of Gold Nanoparticles Bearing the Bioconjugation,” Phys Chem Chem Phys, Vol. 8, 2006, p. 3895.
- G. J. Kearns, E. W. Foster and J. E. Hutchison, “Substrates for Direct Imaging of Chemically Functionalized SiO2 Surfaces by Transmission Electron Microscopy,” Anal Chem, Vol. 78, 2006, p. 298.
- P. Mulvaney, “Surface Plasmon Spectroscopy of Nanosized Metal Particles Langmuir,” Vol. 12, 1996, p. 788.