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Structural Elucidation of Condensed Tannin from <i>Picea mariana</i> Bark
Service de Recherche et d’Expertise en Transformation des Produits Forestiers (SEREX), Amqui, Canada
Département de Génie Chimique, Faculté des Sciences et de Génie, Université Laval, Québec City, Canada
Centre de Recherche sur le Bois, Faculté de Foresterie, de Géographie et de Géomatique, Université Laval, Québec City, Canada
Centre de Recherche sur le Bois, Faculté de Foresterie, de Géographie et de Géomatique, Université Laval, Québec City, Canada
Institut des Nutraceutiques et des Aliments Fonctionnels, Université Laval, Québec City, Canada
Centre de Recherche sur le Bois, Faculté de Foresterie, de Géographie et de Géomatique, Université Laval, Québec City, Canada
- 1 Service de Recherche et d’Expertise en Transformation des Produits Forestiers (SEREX), Amqui, Canada
- 2 Département de Génie Chimique, Faculté des Sciences et de Génie, Université Laval, Québec City, Canada
- 3 Centre de Recherche sur le Bois, Faculté de Foresterie, de Géographie et de Géomatique, Université Laval, Québec City, Canada
- 4 Centre de Recherche sur le Bois, Faculté de Foresterie, de Géographie et de Géomatique, Université Laval, Québec City, Canada
- 5 Institut des Nutraceutiques et des Aliments Fonctionnels, Université Laval, Québec City, Canada
- 6 Centre de Recherche sur le Bois, Faculté de Foresterie, de Géographie et de Géomatique, Université Laval, Québec City, Canada
Journal of Biomaterials and Nanobiotechnology·Volume 04 (2013)·Pages 1–8·Published 26 June 2013·DOI10.4236/jbnb.2013.43A001
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Abstract
The aim of this work was to determine the structural characteristics of the condensed tannins isolated from the aqueous extract of black spruce ( Picea mariana Mill) bark. This is the first report on the structural characterization of condensed tannins from black spruce bark. The condensed tannins from the hot water extract prepared from Picea mariana bark were isolated by column chromatography on Sephadex LH-20 media. In this study, UV-visible and 13 C-NMR spectroscopic techniques, along with pyrolysis-gas chromatography/mass spectrometry (Py-GC/MS) and liquid chromatogramphy analyses were applied to determine the structural characteristics of black spruce bark tannins. We have confirmed for the first time the presence of methoxylated B-rings in the flavanol units of condensed tannins from black spruce bark, which could be regarded as further contribution to the chemotaxonomy of the genus Picea . The methoxylation of Norway spruce bark condensed tannins have been demonstrated previously.
Keywords<i>Picea mariana</i>BarkCondensed TanninsProanthocyanidinsPolyphenolsStructural Characterization
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