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Nanodiamond-Based Tests Systems for Biochemical Determination of Glucose and Cholesterol
Institute of Biophysics SB RAS, Krasnoyarsk, Russia; Siberian Federal University, Krasnoyarsk, Russia.
Institute of Biophysics SB RAS, Krasnoyarsk, Russia; Siberian Federal University, Krasnoyarsk, Russia.
Institute of Biophysics SB RAS, Krasnoyarsk, Russia; Siberian Federal University, Krasnoyarsk, Russia.
Institute of Biophysics SB RAS, Krasnoyarsk, Russia; Siberian Federal University, Krasnoyarsk, Russia.
Institute of Biophysics SB RAS, Krasnoyarsk, Russia; Siberian Federal University, Krasnoyarsk, Russia.
- 1 Institute of Biophysics SB RAS, Krasnoyarsk, Russia; Siberian Federal University, Krasnoyarsk, Russia.
- 2 Institute of Biophysics SB RAS, Krasnoyarsk, Russia; Siberian Federal University, Krasnoyarsk, Russia.
- 3 Institute of Biophysics SB RAS, Krasnoyarsk, Russia; Siberian Federal University, Krasnoyarsk, Russia.
- 4 Institute of Biophysics SB RAS, Krasnoyarsk, Russia; Siberian Federal University, Krasnoyarsk, Russia.
- 5 Institute of Biophysics SB RAS, Krasnoyarsk, Russia; Siberian Federal University, Krasnoyarsk, Russia.
Journal of Biomaterials and Nanobiotechnology·Volume 04 (2013)·Pages 242–246·Published 26 June 2013·DOI10.4236/jbnb.2013.43030
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Abstract
Model test-systems for biochemical determination of glucose and cholesterol are designed by covalent coupling of en zymes to the surface of modified nanodiamonds (MND). In both cases after immobilization on MND the enzymes ex hibit activity and catalyze the formation of a colored product in the chain of successive enzymatic reactions of oxidative azocoupling ( Н 2 О 2 —4-aminoantipyrine—phenol). Produced MND-enzymes complexes: function in DI water and vari ous buffers, over a wide range of temperature and pH, provide for the linear product yield over a broad concentration range of analyzed substances, make possible to test cholesterol and glucose in vitro many times, exhibit functional ac tivity after long storage at + 4°C.
KeywordsNanodiamondTest-SystemGlucoseCholesterol
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