Heat-Resistant Properties of <i>&#945</i>-Chymotrypsin Adsorbed onto Biomass Charcoal Powder

Abstract

Biomass charcoal powder (BCP) was used as a carrier for immobilization of α -chymotrypsin through adsorption. BCP was derived from plant biomass wastes such as dumped bamboos by oxygen-free pyrolysis at low temperatures and grinding with a jet mill. The activity of adsorbed α -chymotrypsin was strongly dependent upon the kind of BCP. The thermal denaturation curve of adsorbed α -chymotrypsin was shifted to high temperature, compared to that of free one. When α -chymotrypsin adsorbed onto BCP of bamboos was incubated at 45 ° C , the half-life of adsorbed α -chymotrypsin was 2.6 times greater than that of free one. After incubation at 45 ° C , the remaining activity of adsorbed α -chymotrypsin markedly depended on the kind of BCP.