Functionalized Asymmetric Poly (Lactic Acid)/Gelatin Composite Membrane for Guided Periodontal Tissue Regeneration
- 1 School of Biomedical Engineering and Med-X Research Institution, Shanghai Jiao Tong University, Shanghai, China
- 2 School of Biomedical Engineering and Med-X Research Institution, Shanghai Jiao Tong University, Shanghai, China
- 3 School of Biomedical Engineering and Med-X Research Institution, Shanghai Jiao Tong University, Shanghai, China
- 4 School of Biomedical Engineering and Med-X Research Institution, Shanghai Jiao Tong University, Shanghai, China
Abstract
Aim: Periodontitis is caused by chronic gingival inflammation and affects a large population in the world. Although guided tissue regeneration (GTR) therapy has been proven to be an effective treatment, the deficiency in the symme trical design of all the GTR membrane in the market leaves large space for im provement. Therefore, we designed a novel asymmetrical bi-layer PLA/gelatin composite membrane for treating periodontitis. Methods: The PLA side was fabricated by electrospinning with metronidazole (MNA) pre-mixed with the PLA solution. The gelatin side containing bioglass (BG) 45S5 was fabricated with freeze-drying process and cross-linked with PLA membrane. The bio-compatibility of the membrane was evaluated in vitro using NIH3T3 cells. The releasing of MNA was measured by spectrophotometer. The bioactivity of the membrane was evaluated by hydroxyapatite (HA) deposit and determined by FTIR spec trometer. The ionic concentration of Ca 2+ and was measured by ICPOES. The expression of the osteogenesis makers was determined by qRT-PCR. Results: The bi-layer PLA/gelatin composite membrane is biocompatible and bioactive. The releasing of MNA can rapidly reach the anti-bacterial effective concentration. Interestingly, the incorporation of MNA modulated the degradation rate of PLA scaffold to meet the requirement of tissue regeneration. Meanwhile, the embedding of the BG powder in the gelatin porous layer provided a favorable Ca 2+ and ion environment for the re generation of the alveolar bone tissue. Conclusions: Taken together, this bi-layer GTR membrane is closer to the physiological structure of the periodontal. The addition of MNA and BG makes it more powerful in treating periodontitis. Moreover, this research provides an example of biomimetic design in fabricating biomaterial for clinical applications.
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