Cytotoxicity Study of Gold Nanoparticles on the Basal-Like Triple-Negative HCC-1937 Breast Cancer Cell Line — Oak Academic Publishing
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Cytotoxicity Study of Gold Nanoparticles on the Basal-Like Triple-Negative HCC-1937 Breast Cancer Cell Line
Université Clermont Auvergne, Institut Universitaire de Technologie, UMR 6533 CNRS/IN2P3, LPC+, Equipe Physico Chimie des Surfaces Nanostructurées, Clermont-Ferrand, France
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Université Clermont Auvergne, Institut Universitaire de Technologie, UMR INSERM 1240 Imagerie Moléculaire et Stratégies Théranostiques, Groupe “Résistance”, Clermont-Ferrand, France
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Université Clermont Auvergne, Institut Universitaire de Technologie, UMR 6533 CNRS/IN2P3, LPC+, Equipe Physico Chimie des Surfaces Nanostructurées, Clermont-Ferrand, France
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Université Clermont Auvergne, Institut Universitaire de Technologie, UMR INSERM 1240 Imagerie Moléculaire et Stratégies Théranostiques, Groupe “Résistance”, Clermont-Ferrand, France
,
Université Clermont Auvergne, Institut Universitaire de Technologie, UMR 6533 CNRS/IN2P3, LPC+, Equipe Physico Chimie des Surfaces Nanostructurées, Clermont-Ferrand, France
,
Université Clermont Auvergne, Institut Universitaire de Technologie, UMR INSERM 1240 Imagerie Moléculaire et Stratégies Théranostiques, Groupe “Résistance”, Clermont-Ferrand, France
,
Université Clermont Auvergne, Institut Universitaire de Technologie, UMR INSERM 1240 Imagerie Moléculaire et Stratégies Théranostiques, Groupe “Résistance”, Clermont-Ferrand, France
,
Université Clermont Auvergne, Institut Universitaire de Technologie, UMR 6533 CNRS/IN2P3, LPC+, Equipe Physico Chimie des Surfaces Nanostructurées, Clermont-Ferrand, France
1 Université Clermont Auvergne, Institut Universitaire de Technologie, UMR 6533 CNRS/IN2P3, LPC+, Equipe Physico Chimie des Surfaces Nanostructurées, Clermont-Ferrand, France
2 Université Clermont Auvergne, Institut Universitaire de Technologie, UMR INSERM 1240 Imagerie Moléculaire et Stratégies Théranostiques, Groupe “Résistance”, Clermont-Ferrand, France
3 Université Clermont Auvergne, Institut Universitaire de Technologie, UMR 6533 CNRS/IN2P3, LPC+, Equipe Physico Chimie des Surfaces Nanostructurées, Clermont-Ferrand, France
4 Université Clermont Auvergne, Institut Universitaire de Technologie, UMR INSERM 1240 Imagerie Moléculaire et Stratégies Théranostiques, Groupe “Résistance”, Clermont-Ferrand, France
5 Université Clermont Auvergne, Institut Universitaire de Technologie, UMR 6533 CNRS/IN2P3, LPC+, Equipe Physico Chimie des Surfaces Nanostructurées, Clermont-Ferrand, France
6 Université Clermont Auvergne, Institut Universitaire de Technologie, UMR INSERM 1240 Imagerie Moléculaire et Stratégies Théranostiques, Groupe “Résistance”, Clermont-Ferrand, France
7 Université Clermont Auvergne, Institut Universitaire de Technologie, UMR INSERM 1240 Imagerie Moléculaire et Stratégies Théranostiques, Groupe “Résistance”, Clermont-Ferrand, France
8 Université Clermont Auvergne, Institut Universitaire de Technologie, UMR 6533 CNRS/IN2P3, LPC+, Equipe Physico Chimie des Surfaces Nanostructurées, Clermont-Ferrand, France
The Triple Negative “Basal-like” breast cancer (TNBL) tumours have a high proliferative capacity and develop a resistance phenotype associated with me tastases. However, the management of TNBL carcinomas is still not stan dardized. Among the promising trails, gold nanoparticles could be a relevant tool for the development of a targeted treatment for this breast cancer subtype in monotherapy, associated and/or conjugated with other drugs. In this work, we report the cytotoxicity impact of gold nanoparticles wrapped in Poly-Ethylene Glycol (PEG) on the TNBL HCC-1937 breast cancer cell line . PEG-coated gold nanoparticles (PEG-Au NPs) were synthesized by a two-step method using a reduction process followed by a post - functionalization called PEGylation. PEG-Au NPs were characterized using transmission electron mi croscopy and X-ray diffraction. The gold content of the samples was deter mined using atomic absorption spectrometer. The cytotoxicity tests wer e performed using Sulforhodamine B survival test and resazurin viability test. PEG-Au NPs impact analysis on HCC1937 TNBL cell line showed a clear toxic action of type dose dependent and at long term. These PEGylated gold nanoparticles present a promising tool for the development of tumor-specific radi osensitizing vectors, with or without the association of other treatment strategies.
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