The application of electric field to graft materials has significant contribution in bone healing mechanism. Hence, the aim of this study is to develop conductive hydroxyapatite (HAp) scaffolds by introducing different concentrations of silver ion into its structure and demonstrate its impact on in vitro bioactivity and electrical properties. Hydroxyapatite was synthesized by wet chemical method and calcium ions from HAp structure have been partially replaced by silver ions. The HAp and Ag-HAp nanocomposites were characterized by Fourier-transform infrared, Raman spectroscopy, XRD and EDAX for functional group and phase formation analysis as well as to confirm existence of silver ions in HAp structure respectively. Bioactivity of these scaffolds was assessed by using simulated body fluid. The surface morphology, structural analysis and electrical properties of scaffolds before and after formation of newly calcified tissues on its surface were examined via scanning electron microscopy (SEM), XRD, FTIR, dielectric and impedance spectroscopy techniques. Overall, our finding suggests that the administration of silver ions in HAp scaffold boosts bioactivity and has strong correlation with electrical properties.
KeywordsHydroxyapatiteWet Chemical MethodBioactivityElectrical Properties
Mondal, S., Pal, U. and Dey, A. (2016) Natural Origin Hydroxyapatite Scaffold as Potential Bone Tissue Engineering Substitute. Ceramic International, 42, 18338-18346. https://doi.org/10.1016/j.ceramint.2016.08.165
Pon-On, W., Suntornsaratoon, P., Charoenphandhu, N., Thongbunchoo, J., Karishnamra, N. and Tang, I.M. (2016) hydroxyapatite from Fish Scale for Potential Use as Bone Scaffold or Regenerative Material. Material Science and Engineering C, 62, 183-189. https://doi.org/10.1016/j.msec.2016.01.051
Mondal, S., Hoang, G., Manivasagan, P., Moorthy, M.S., Kim, H.H., Phan, T.T.V. and Oh, J. (2019) Comparative Characterization of Biogenic and Chemical Synthesized Hydroxyapatite Biomaterials for Potential Biomedical Application. Material Chemistry and Physics, 228, 344-356. https://doi.org/10.1016/j.matchemphys.2019.02.021
Zhang, K., Zeng, K., Shen, C., Tian, S. and Yang, M. (2018) Determination of Protein Kinase A Activity and Inhibition by Using Hydroxyapatite Nanoparticles as a Fluorescent Probe. Mikrochimica Acta, 185, 225. https://doi.org/10.1007/s00604-018-2754-1
Verma, A.H., Sampath Kumar, T.S., Madhumathi, K., Rubaiya, Y., Ramalingan, M. and Doble, M. (2019) Curcumin Releasing Eggshell Derived Carbonated Apatite Nanocarriers for Combined Anti-Cancer, Anti-Inflammatory and Bone Regenerative Therapy. Journal of Nanoscience and Nanotechnology, 19, 6872-6880. https://doi.org/10.1166/jnn.2019.16640
Zhao, L., Zhao, W., Liu, Y., Chen, X. and Wang, Y. (2017) Nano-Hydroxyapatite-Derived Drug and Gene Co-Delivery System for Anti-Angiogenesis Therapy of Breast Cancer. Medical Science Monitor, 23, 4723-4732. https://doi.org/10.12659/MSM.902538
Li, M., Xiong, P., Yan, F., Li, S., Ren, C., Yin, Z., Li, A., Li, H., Ji, X., Zheng, Y. and Cheng, Y. (2018) An Overview of Graphene-Based Hydroxyapatite Composites for Orthopaedic Applications. Bioactive Materials, 3, 1-18. https://doi.org/10.1016/j.bioactmat.2018.01.001
Yu, W., Sun, T.W., Ding, Z., Qi, C., Zhao, H., Chen, F., Shi, Z., Zhu, Y.J., Chen, D. and He, Y. (2017) Copper-Doped Mesoporous Hydroxyapatite Microspheres Synthesized by a Microwave-Hydrothermal Method Using Creatine Phosphate as an Organic Phosphorus Source: Application in Drug Delivery and Enhanced Bone Regeneration. Journal of Materials Chemistry B, 5, 1039-1052. https://doi.org/10.1039/C6TB02747D
Mahabole, M., Bahir, M. and Khairnar, R. (2013) Mn Blended Hydroxyapatite Nanoceramic: Bioactivity, Dielectric and Luminescence Studies. Journal of Biomimetics, Biomaterials and Tissue Engineering, 18, 43-59. https://doi.org/10.4028/www.scientific.net/JBBTE.18.43
Electrical Properties of Newly Calcified Tissues on the Surface of Silver Ion Administrated Hydroxyapatite Scaffolds — Oak Academic Publishing
Kim, B., Yang, S., Yoon, J. and Lee, J. (2017) Enhanced Bone Regeneration by Silicon-Substituted Hydroxyapatite Derived from Cuttlefish Bone. Clinical Oral Implants Research, 28, 49-56. https://doi.org/10.1111/clr.12613
Tsai, S.W., Yu, W.X., Hwang, P.A., Huang, S.S., Lin, H.M., Hsu, Y.W. and Hsu, F.Y. (2018) Fabrication and Characterization of Strontium-Substituted Hydroxyapatite-CaO-CaCO 3 Nanofibers with a Mesoporous Structure as Drug Delivery Carriers. Pharmaceutics, 10, 179. https://doi.org/10.3390/pharmaceutics10040179
Frasnelli, M., Cristofaro, F., Sglavo, V.M., Dire, S., Callonea, E., Ceccato, R., Bruni, G., Cornaglia, A.I. and Visai, L. (2017) Synthesis and Characterization of Strontium-Substituted Hydroxyapatitenanoparticles for Bone Regeneration. Materials Science and Engineering C, 71, 653-662. https://doi.org/10.1016/j.msec.2016.10.047
Kargozar, S., Lotfibakhshaiesh, N., Ai, J., Mozafari, M., Milan, P.B., Hamzehlou, S., Barati, M., Baino, F., Hill, R.G. and Joghataei, M.T. (2017) Strontium- and Cobalt-Substituted Bioactive Glasses Seeded with Human Umbilical Cord Perivascular Cells to Promote Bone Regeneration via Enhanced Osteogenic and Angiogenic Activities. Acta Biomaterialia, 58, 502-514. https://doi.org/10.1016/j.actbio.2017.06.021
Yu, W., Sun, T.W., Qi, C., Ding, Z., Zhao, H., Zhao, S., Shi, Z., Zhu, Y.J., Chen, D. and He, Y. (2017) Evaluation of Zinc-Doped Mesoporous Hydroxyapatite Microspheres for the Construction of a Novel Biomimetic Scaffold Optimized for Bone Augmentation. International Journal of Nanomedicine, 12, 2293-2306. https://doi.org/10.2147/IJN.S126505
Qiao, H., Song, G., Huang, Y., Yang, H., Han, S., Zhang, X., Wang, Z., Ma, J., Bu, X. and Fu, L. (2019) Si, Sr, Ag Co-Doped Hydroxyapatite/TiO 2 Coating: Enhancement of Its Antibacterial Activity and Osteoinductivity. RSC Advances, 9, 13348-13364. https://doi.org/10.1039/C9RA01168D
Rodriguez, D.A.L., de Lima, R., Fraceto, L.F., Perez, A.L., Dominguez, M.B., Batres, R.G., Rojas, A.R. and Carmona, V.O. (2017) Development of HA/Ag-NPs Composite Coating from Green Process for Hip Applications. Molecules, 22, 1291. https://doi.org/10.3390/molecules22081291
Akiyama, T., Miyamoto, H., Yonekura, Y., Tsukamoto, M., Ando, Y., Noda, I., Sonohata, M. and Mawatari, M. (2013) Silver Oxide-Containing Hydroxyapatite Coating Has in Vivo Antibacterial Activity in the Rat Tibia. Journal of Orthopaedic Research, 31, 1195-1200. https://doi.org/10.1002/jor.22357
Fonseca, F.M., Costa, A.M., Campos, J.B., LSB Marcal, R., da Rocha, D.N. and da Silva, M.H. (2017) Bioactivity Assessment of Ag-HA. Biomaterials and Medical Applications, 1, 2. https://doi.org/10.4172/2577-0268.1000106
Yan, Y., Zhang, X., Huang, Y., Ding, Q. and Pang, X. (2014) Antibacterial and Bioactivity of Silver Substituted Hydroxyapatite/TiO 2 Nanotube Composite Coatings on Titanium. Applied Surface Science, 314, 348-357. https://doi.org/10.1016/j.apsusc.2014.07.027
Erakovic, S., Jankovic, A., Veljovic, D., Palcevskis, E., Mitric, M., Stevanovic, T., Janackovic, D. and Stankovic, V.M. (2013) Corrosion Stability and Bioactivity in Simulated Body Fluid of Silver/Hydroxyapatite and Silver/Hydroxyapatite/Lignin Coatings on Titanium Obtained by Electrophoretic Deposition. The Journal of Physical Chemistry B, 117, 1633-1643. https://doi.org/10.1021/jp305252a
Sivolella, S., Stellini, E., Brunello, G., Gardin, C., Ferroni, L., Bressan, E. and Zavan, B. (2012) Silver Nanoparticles in Alveolar Bone Surgery Devices. Journal of Nanomaterials, 2012, Article ID: 975842. https://doi.org/10.1155/2012/975842
Diago, P., S. Bielsa, J.M. and G. Escoda, C. (2001) Periapical Surgery of 31 Lower Molars Based on the Ultrasound Technique and Retrograde Filling with Silver Amalgam. Medicina Oral, Patología Oral y Cirugía Bucal, 6, 376-382.
Sierpowska, J., Toyras, J., Hakulinen, M.A., Saarakkala, S., Jurvelin, J.S. and Lappalainen, R. (2003) Electrical and Dielectric Properties of Bovine Trabecular Bone-Relationships with Mechanical Properties and Mineral Density. Physics in Medicine & Biology, 48, 775-786. https://doi.org/10.1088/0031-9155/48/6/306
Balmer, T.W., Vesztergom, S., Broekmann, P., Staheland, A. and Buchler, P. (2018) Characterization of the Electrical Conductivity of Bone and Its Correlation to Osseous Structure. Scientific Reports, 8, 8601. ttps://doi.org/10.1038/s41598-018-26836-0
Petrov, I., Kalinkevich, O., Pogorielov, M., Kalinkevich, A., Stanislavov, A., Sklyar, A., Danilchenko, S. and Yovcheva, T. (2016) Dielectric and Electric Properties of New Chitosan-Hydroxyapatite Materials for Biomedical Application: Dielectric Spectroscopy, and Corona Treatment. Carbohydrate Polymers, 151, 770-778. https://doi.org/10.1016/j.carbpol.2016.05.110
Shayesteh, Y.S., Eslami, B., Dehghan, M.M., Vaziri, H., Alikhassi, M., Mangoli, A. and Khojasteh, A. (2007) The Effect of a Constant Electrical Field on Osseointegration after Immediate Implantation in Dog Mandibles: A Preliminary Study. Journal of Prosthodontics, 16, 337-342. https://doi.org/10.1111/j.1532-849X.2007.00208.x
Nakamura, S., Kobayashi, T., Nakamura, M., Itoh, S. and Yamashita, K. (2010) Electrostatic Surface Charge Acceleration of Bone Ingrowth of Porous Hydroxyapatite/β-Tricalcium Phosphate Ceramics. Journal of Biomedical Materials Research Part A, 92A, 267-275. https://doi.org/10.1002/jbm.a.32354
Huegel, J., Choi, D.S. and Nuss, C.A. (2018) Effects of Pulsed Electromagnetic Field Therapy at Different Frequencies and Durations on Rotator Cuff Tendon-to-Bone Healing in a Rat Model. Journal of Shoulder and Elbow Surgery, 27, 553-560. https://doi.org/10.1016/j.jse.2017.09.024
Midura, R.J., Ibiwoye, M.O. and Powell, K.A. (2005) Pulsed Electromagnetic Field Treatments Enhance the Healing of Fibular Osteotomies. Journal of Orthopaedic Research, 23, 1035-1046. https://doi.org/10.1016/j.orthres.2005.03.015
Lin, M.C., Yang, F., Herfat, S.T., Bahney, C.S., Marmor, M. and Maharbiz, M.M. (2017) New Opportunities for Fracture Healing Detection: Impedance Spectroscopy Measurements Correlate to Tissue Composition in Fractures. Journal of Orthopaedic Research, 35, 2620-2629. https://doi.org/10.1002/jor.23570
Kozhevnikov, E., Hou, X., Qiao, S., Zhao, Y., Li, C. and Tian, W. (2016) Electrical Impedance Spectroscopy-a Potential Method for the Study and Monitoring of a Bone Critical-Size Defect Healing Process Treated with Bone Tissue Engineering and Regenerative Medicine Approaches. Journal of Materials Chemistry B, 4, 2757-2767. https://doi.org/10.1039/C5TB02707A
Chavan, P.N., Bahir, M.M., Mene, R.U., Mahabole, M.P. and Khairnar, R.S. (2010) Study of Nanobiomaterial Hydroxyapatite in Simulated Body Fluid: Formation and Growth of Apatite. Materials Science and Engineering B, 168, 224-230. https://doi.org/10.1016/j.mseb.2009.11.012
Mahabole, M.P., Bahir, M.M., Kalyankar, N.V. and Khairnar, R.S. (2012) Effect of Incubation in Simulated Body Fluid on Dielectric and Photoluminescence Properties of Nano-Hydroxyapatite Ceramic Doped with Strontium Ions. Journal of Biomedical Science and Engineering, 5, 396-405. https://doi.org/10.4236/jbise.2012.57050
Jalota, S., Bhaduri, S.B. and Tas, A.C. (2008) Using a Synthetic Body Fluid (SBF) Solution of 27 mM HCO 3 − to Make Bone Substitutes More Osteointegrative. Material Science and Engineering C, 28, 129-140. https://doi.org/10.1016/j.msec.2007.10.058
Antonakos, A., Liarokapis, E. and Leventouri, T. (2007) Micro-Raman and FTIR Studies of Synthetic and Natural Apatites. Biomaterials, 28, 3043-3054. https://doi.org/10.1016/j.biomaterials.2007.02.028
Liu, L., Wu, Y., Xu, C., Yu, S., Wu, X. and Dai, H. (2018) Synthesis, Characterization of Nano-β-Tricalcium Phosphate and the Inhibition on Hepatocellular Carcinoma Cells. Journal of Nanomaterials, 2018, Article ID: 6468246. https://doi.org/10.1155/2018/6468246