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Visualization of decreased docosahexaenoic acid in the hippocampus of rats fed an n – 3 fatty acid-deficient diet by imaging mass spectrometry
Japan Advanced Institute of Science and Technology, Ishikawa, Japan
Shimane University Haculty of Medicine, Shimane, Japan
Bruker Daltonics K.K., Kanagawa, Japan
Shimane University Haculty of Medicine, Shimane, Japan
- 1 Japan Advanced Institute of Science and Technology, Ishikawa, Japan
- 2 Shimane University Haculty of Medicine, Shimane, Japan
- 3 Bruker Daltonics K.K., Kanagawa, Japan
- 4 Shimane University Haculty of Medicine, Shimane, Japan
Journal of Biophysical Chemistry·Volume 03 (2012)·Pages 221–226·Published 28 August 2012·DOI10.4236/jbpc.2012.33025
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Abstract
The present study employed an imaging mass spectrometry (IMS) method to evaluate the effect of dietary n – 3 fatty acids on the fatty acid composition in rat brain. Rats were divided into two groups and fed either an n – 3 fatty acid-deficient or adequate diet. We determined the decreased n – 3 fatty acids in the hippocampus of rats fed an n – 3 fatty acid-deficient diet compared to the control. IMS visualization was achieved at a resolution of 100 m in rat brain, and showed decreased docosahexaenoic acid (DHA)-containing phosphatidyl choline (PC) or phosphatidyl ethanolamine (PE) in the hippocampus of rats fed an n – 3 fatty acid-deficient diet.
KeywordsDocosahexaenoic AcidImaging Mass SpectrometryFourier-Transform Ion Cyclotron Resonance Mass SpectrometryHippocampus
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