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Molecular basis of scavenging effect of zonisamide on hydroxyl radical <i>in vitro</i>

¹ Department of Clinical Pharmacy, Shujitsu University School of Pharmacy, Nishigawara, Japan
² Department of Clinical Pharmacy, Shujitsu University School of Pharmacy, Nishigawara, Japan
³ Department of Clinical Pharmacy, Shujitsu University School of Pharmacy, Nishigawara, Japan
⁴ Department of Bacteriology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Shikatacho, Japan
⁵ Department of Neurology, Saint Louis University School of Medicine, St. Louis, USA
⁶ Department of Brain Science, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Shikatacho, Japan

Journal of Biophysical Chemistry·Volume 03 (2012)·Pages 256–258·Published 28 August 2012·DOI10.4236/jbpc.2012.33030

Abstract

Zonisamide (ZNS), a commonly used anticonvulsant, scavenged hydroxyl radicals at a clinically relevant concentration. Reactants of ZNS with hydrogen peroxide and with hydrogen peroxide plus UV irradiation, yielding hydroxyl radicals, were analyzed by the LC/MS technique. Many small fragments were found in the both reactions, suggesting that ZNS was decomposed not only by hydroxyl radicals but also by hydrogen peroxide. Furthermore, mass-fragment-grams showed that m/z: 213 (ZNS itself) was decreased markedly and m/z: 118 (may be a decomposed product by ring cleavage of ZNS) was detected specifically by treatment with hydroxyl radical. These data suggested that ZNS may react directly with free radicals.

KeywordsZonisamideHydroxyl RadicalsLC/MSESR

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Molecular basis of scavenging effect of zonisamide on hydroxyl radical <i>in vitro</i>

Abstract

References (9)