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Structural Change in Dimyristoyl Phosphatidyl Choline (DMPC) Molecule Alkyl Chains on the Addition of Cholesterol to DMPC Monolayer: Specific Addition Effect of Cholesterol
Department of Life Science and Applied Chemistry, Graduate School of Engineering, Nagoya Institute of Technology, Nagoya, Aichi, Japan
Department of Life Science and Applied Chemistry, Graduate School of Engineering, Nagoya Institute of Technology, Nagoya, Aichi, Japan
Department of Life Science and Applied Chemistry, Graduate School of Engineering, Nagoya Institute of Technology, Nagoya, Aichi, Japan
Department of Life Science and Applied Chemistry, Graduate School of Engineering, Nagoya Institute of Technology, Nagoya, Aichi, Japan
Nanomaterials Production Division, Food and Energy Security Research and Product Center, Sendai, Japan
Department of Chemistry, School of Arts and Sciences, Showa University, Kamiyoshida Fujiyoshida, Yamanashi, Japan
- 1 Department of Life Science and Applied Chemistry, Graduate School of Engineering, Nagoya Institute of Technology, Nagoya, Aichi, Japan
- 2 Department of Life Science and Applied Chemistry, Graduate School of Engineering, Nagoya Institute of Technology, Nagoya, Aichi, Japan
- 3 Department of Life Science and Applied Chemistry, Graduate School of Engineering, Nagoya Institute of Technology, Nagoya, Aichi, Japan
- 4 Department of Life Science and Applied Chemistry, Graduate School of Engineering, Nagoya Institute of Technology, Nagoya, Aichi, Japan
- 5 Nanomaterials Production Division, Food and Energy Security Research and Product Center, Sendai, Japan
- 6 Department of Chemistry, School of Arts and Sciences, Showa University, Kamiyoshida Fujiyoshida, Yamanashi, Japan
Journal of Biophysical Chemistry·Volume 10 (2019)·Pages 15–29·Published 28 May 2019·DOI10.4236/jbpc.2019.102002
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Abstract
We have investigated the morphology of dimyristoyl phosphatidyl choline (DM PC)—cholesterol mixed monolayer formed on the water surface by drop ping method using surface tension measurement (STm), Brewster angle microscopy (BAM), and infrared external reflection spectroscopy (IERS). STm results showed negative deviation of the limiting molecular area ( A 0 ) of cholesterol occurred when cholesterol was added to the DMPC monolayer. BAM images showed the expandable DMPC monolayer changed to the condensed rigid monolayer at more than cholesterol mole fraction ( x Chol ) 0.4. IERS recordings showed that the addition of cholesterol at x Chol = 0.4 occurred structural change from gauche - to trans - conformation of two DMPC molecule alkyl chains. From these results, it is found that cholesterol molecule has sp ecific properties that cause structural transition of DMPC molecule alkyl chains.
KeywordsDimyristoyl Phosphatidyl Choline (DMPC)CholesterolSurface Tension Measurement (STm)Brewster Angle Microscopy (BAM)Infrared External Reflection Microscopy (IERS)Structural Transition
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