Potential Application of a Wine Extract in Skin Care: How to Benefit from the Antibacterial, Antioxidant and Elastase Inhibiting Properties — Oak Academic Publishing
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Potential Application of a Wine Extract in Skin Care: How to Benefit from the Antibacterial, Antioxidant and Elastase Inhibiting Properties
Since plant polyphenols have many beneficial properties on health, the aim of this study was to evaluate the potential use of a phenolic wine extract, a by-product of wine production, for skin care on HaCaT cells. In these studies, a significant reduction of reactive oxygen species formation in HaCaT cells and severe elastase inhibition was observed. In contrast, the wine extract caused a major increase in lipase activity. The extract showed no influence on cell proliferation, but an immunomodulatory effect on the release of the interleukins IL-6 and IL-8 was found. The phenolic wine extract demonstrated a strong activity against gram-positive and gram-negative pathogens, yeasts, and fungi. Overall, our results show that the investigated phenolic wine extract is a promising ingredient for anti-aging skin care, could contribute to the improvement of skin appearance and health, and may positively affect cellulite.
Goebeler, M. and Hamm, H. (2017) Basiswissen Dermatologie. Springer Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-52811-2
Fritsch, P. and Thomas, S. (2018) Dermatologie Venerologie. Springer, Berlin. https://doi.org/10.1007/978-3-662-53647-6
Worret, W.-I. (2004) Kosmetische Dermatologie. In: Fallstricke und Fehlerquellen in der Dermatologie, Springer, Vienna, 265-274. https://doi.org/10.1007/978-3-7091-0577-1_14
Salmon, J.K., Armstrong, C.A. and Ansel, J.C. (1994) The Skin as an Immune Organ. The Western Journal of Medicine, 160, 146-152.
Piktel, E., et al. (2019) Inhibition of Inflammatory Response in Human Keratinocytes by Magnetic Nanoparticles Functionalized with PBP10 Peptide Derived from the PIP2-Binding Site of Human Plasma Gelsolin. Journal of Nanobiotechnology, 17, Article No. 22. https://doi.org/10.1186/s12951-019-0455-5
Ghadially, R., Brown, B.E., Sequeira-Martin, S.M., Feingold, K.R. and Elias, P.M. (1995) The Aged Epidermal Permeability Barrier. Structural, Functional, and Lipid Biochemical Abnormalities in Humans and a Senescent Murine Model. The Journal of Clinical Investigation, 95, 2281-2290. https://doi.org/10.1172/JCI117919
Leyden, J.J. (1990) Clinical Features of Ageing Skin. British Journal of Dermatology, 122, 1-3. https://doi.org/10.1111/j.1365-2133.1990.tb16118.x
Braverman, I.M. and Fonferko, E. (1982) Studies in Cutaneous Aging: I. The Elastic Fiber Network. Journal of Investigative Dermatology, 78, 434-443. https://doi.org/10.1111/1523-1747.ep12507866
Thakur, R., Batheja, P., Kaushik, D. and Michniak, B. (2009) Chapter 4-Structural and Biochemical Changes in Aging Skin and Their Impact on Skin Permeability Barrier. In: Dayan, N., Ed., Skin Aging Handbook, Elsevier, Amsterdam, 55-90. https://doi.org/10.1016/B978-0-8155-1584-5.50008-9
Nagase, H., Visse, R. and Murphy, G. (2006) Structure and Function of Matrix Metalloproteinases and TIMPs. Cardiovascular Research, 69, 562-573. https://doi.org/10.1016/j.cardiores.2005.12.002
Sárdy, M. (2009) Role of Matrix Metalloproteinases in Skin Ageing. Connective Tissue Research, 50, 132-138. https://doi.org/10.1080/03008200802585622
Bergfeld, W.F. (1997) The Aging Skin. International Journal of Fertility and Women’s Medicine, 42, 57-66.
Matsumura, Y. and Ananthaswamy, H.N. (2004) Toxic Effects of Ultraviolet Radiation on the Skin. Toxicology and Applied Pharmacology, 195, 298-308. https://doi.org/10.1016/j.taap.2003.08.019
Wurm, E.M.T., Longo, C., Curchin, C., Soyer, H.P., Prow, T.W. and Pellacani, G. (2012) In Vivo Assessment of Chronological Ageing and Photoageing in Forearm Skin Using Reflectance Confocal Microscopy. British Journal of Dermatology, 167, 270-279. https://doi.org/10.1111/j.1365-2133.2012.10943.x
de Jager, T.L., Cockrell, A.E. and Du Plessis, S.S. (2017) Ultraviolet Light Induced Generation of Reactive Oxygen Species. In: Ahmad, S., Ed., Ultraviolet Light in Human Health, Diseases and Environment, Advances in Experimental Medicine and Biology, Vol. 996, Springer, Cham, 15-23. https://doi.org/10.1007/978-3-319-56017-5_2
Yamamoto, Y. (2001) Role of Active Oxygen Species and Antioxidants in Photoaging. Journal of Dermatological Science, 27, 1-4. https://doi.org/10.1016/S0923-1811(01)00120-7
Bharadvaja, N., Gautam, S. and Singh, H. (2022) Natural Polyphenols: A Promising Bioactive Compounds for Skin Care and Cosmetics. Molecular Biology Reports, 50, 1817-1828. https://doi.org/10.1007/s11033-022-08156-9
Spinei, M. and Oroian, M. (2021) The Potential of Grape Pomace Varieties as a Dietary Source of Pectic Substances. Foods, 10, Article 867. https://doi.org/10.3390/foods10040867
Nigam, P.S.-N. and Pandey, A. (2009) Biotechnology for Agro-Industrial Residues Utilisation. Springer, Dordrecht.
Lee, S.Y., et al. (2010) Treatment of Phenol-Contaminated Soil by Corynebacterium Glutamicum and Toxicity Removal Evaluation. Journal of Hazardous Materials, 182, 937-940. https://doi.org/10.1016/j.jhazmat.2010.06.092
Kalli, E., Lappa, I., Bouchagier, P., Tarantilis, P.A. and Skotti, E. (2018) Novel Application and Industrial Exploitation of Winery By-Products. Bioresources and Bioprocessing, 5, Article No. 46. https://doi.org/10.1186/s40643-018-0232-6
Mosmann, T. (1983) Rapid Colorimetric Assay for Cellular Growth and Survival: Application to Proliferation and Cytotoxicity Assays. Journal of Immunological Methods, 65, 55-63. https://doi.org/10.1016/0022-1759(83)90303-4
International Organization for Standardization (2019) DIN EN ISO 11930 Cosmetics-Microbiology-Evaluation of the Antimicrobial Protection of a Cosmetic Product (ISO/DIS 11930:2019) Beuth, Berlin.
Bieth, J., Spiess, B. and Wermuth, C.G. (1974) The Synthesis and Analytical Use of a Highly Sensitive and Convenient Substrate of Elastase. Biochemical Medicine, 11, 350-357. https://doi.org/10.1016/0006-2944(74)90134-3
Ferrazzano, G.F., Amato, I., Ingenito, A., Zarrelli, A., Pinto, G. and Pollio, A. (2011) Plant Polyphenols and Their Anti-Cariogenic Properties: A Review. Molecules, 16, 1486-1507. https://doi.org/10.3390/molecules16021486
Daglia, M., et al. (2007) Antibacterial Activity of Red and White Wine against Oral Streptococci. Journal of Agricultural and Food Chemistry, 55, 5038-5042. https://doi.org/10.1021/jf070352q
Papadopoulou, C., Soulti, K. and Roussis, I.G. (2005) Potential Antimicrobial Activity of Red and White Wine Phenolic Extracts against Strains of Staphylococcus aureus, Escherichia coli and Candida albicans. Food Technology and Biotechnology, 43, 41-46.
Castilla, P., et al. (2006) Concentrated Red Grape Juice Exerts Antioxidant, Hypolipidemic and Antiinflammatory Effects in both Hemodialysis Patients and Healthy Subjects. The American Journal of Clinical Nutrition, 84, 252-262. https://doi.org/10.1093/ajcn/84.1.252
Chacón, M.R., et al. (2009) Grape-Seed Procyanidins Modulate Inflammation on Human Differentiated Adipocytes in Vitro. Cytokine, 47, 137-142. https://doi.org/10.1016/j.cyto.2009.06.001
Rubilar, M., Pinelo, M., Shene, C., Sineiro, J. and Nuñez, M.J. (2007) Separation and HPLC-MS Identification of Phenolic Antioxidants from Agricultural Residues: Almond Hulls and Grape Pomace. Journal of Agricultural and Food Chemistry, 55, 10101-10109. https://doi.org/10.1021/jf0721996
Rafique, M., et al. (2021) Development of Grape Seed Extract Based Formulations by Using Non-Invasive Biophysical Technique and Its Impact on Skin Aging. Pakistan Journal of Pharmaceutical Sciences, 34, 1621-1628.
Perra, M., et al. (2021) Extraction of the Antioxidant Phytocomplex from Wine-Making by-Products and Sustainable Loading in Phospholipid Vesicles Specifically Tailored for skin Protection. Biomedicine & Pharmacotherapy, 142, Article ID: 111959. https://doi.org/10.1016/j.biopha.2021.111959
Emmulo, E., Ceccantoni, B., Bellincontro, A, and Mencarelli, F. (2021) Use of Water and Ethanol Extracts from Wine Grape Seed Pomace to Prepare an Antioxidant Toothpaste. Journal of the Science of Food and Agriculture, 101, 5813-5818. https://doi.org/10.1002/jsfa.11232
Arican, O., Aral, M., Sasmaz, S. and Ciragil, P. (2005) Serum Levels of TNF-α, IFN-γ, IL-6, IL-8, IL-12, IL-17 and IL-18 in Patients with Active Psoriasis and Correlation with Disease Severity. Mediators of inflammation, 2005, Article ID: 201561. https://doi.org/10.1155/MI.2005.273
Kimata, H. and Lindley, I. (1994) Detection of Plasma Interleukin-8 in Atopic Dermatitis. Archives of Disease in Childhood, 70, 119-122. https://doi.org/10.1136/adc.70.2.119
Johnson, B.Z., Stevenson, A.W., Prêle, C.M., Fear, M.W. and Wood, F.M. (2020) The Role of IL-6 in Skin Fibrosis and Cutaneous Wound Healing. Biomedicines, 8, Article 101. https://doi.org/10.3390/biomedicines8050101
Engelhardt, E., Toksoy, A., Goebeler, M., Debus, S., Bröcker, E.-B. and Gillitzer, R. (1998) Chemokines IL-8, GROα, MCP-1, IP-10 and Mig Are Sequentially and Differentially Expressed During Phase-Specific Infiltration of Leukocyte Subsets in Human Wound Healing. The American Journal of Pathology, 153, 1849-1860. https://doi.org/10.1016/S0002-9440(10)65699-4
Wang, X. and Zhang, Y. (2018) Resveratrol Alleviates LPS-Induced Injury in Human Keratinocyte Cell Line HaCaT by Up-Regulation of miR-17. Biochemical and Biophysical Research Communications, 501, 106-112. https://doi.org/10.1016/j.bbrc.2018.04.184
Tao, K., et al. (2015) Effects of Resveratrol on the Treatment of Inflammatory Response Induced by Severe Burn. Inflammation, 38, 1273-1280. https://doi.org/10.1007/s10753-014-0097-6
Nakanishi, T., Mukai, K., Yumoto, H., Hirao, K., Hosokawa, Y. and Matsuo, T. (2010) Anti-Inflammatory Effect of Catechin on Cultured Human Dental Pulp Cells Affected by Bacteria-Derived Factors. European Journal of Oral Sciences, 118, 145-150. https://doi.org/10.1111/j.1600-0722.2010.00714.x
Terra, X., et al. (2009) Grape-Seed Procyanidins Prevent Low-Grade Inflammation by Modulating Cytokine Expression in Rats Fed a High-Fat Diet. The Journal of Nutritional Biochemistry, 20, 210-218. https://doi.org/10.1016/j.jnutbio.2008.02.005
Kong, L., et al. (2015) Icariin Inhibits TNF-α/IFN-γ Induced Inflammatory Response via Inhibition of the Substance P and p38-MAPK Signaling Pathway in Human Keratinocytes. International Immunopharmacology, 29, 401-407. https://doi.org/10.1016/j.intimp.2015.10.023
Wölfle, U., Hoffmann, J., Haarhaus, B., Mittapalli, R.V. and Schempp, C.M. (2017) Anti-Inflammatory and Vasoconstrictive Properties of Potentilla Erecta—A Traditional Medicinal Plant from the Northern Hemisphere. Journal of Ethnopharmacology, 204, 86-94. https://doi.org/10.1016/j.jep.2017.03.058
Liang, P., et al. (2008) The Role of Peroxisome Proliferator-Activated Receptor-β/δ in Epidermal Growth Factor-Induced HaCaT Cell Proliferation. Experimental Cell Research, 314, 3142-3151. https://doi.org/10.1016/j.yexcr.2008.06.013
Kampa, M., et al. (2004) Antiproliferative and Apoptotic Effects of Selective Phenolic Acids on T47D Human Breast Cancer Cells: Potential Mechanisms of Action. Breast Cancer Research, 6, Article No. R63. https://doi.org/10.1186/bcr752
Holian, O. and Walter, R. (2001) Resveratrol Inhibits the Proliferation of Normal Human Keratinocytes in Vitro. Journal of Cellular Biochemistry, 81, 55-62. https://doi.org/10.1002/jcb.1085
Tenta, R., et al. (2017) Antiproliferative Effects of Red and White Wine Extracts in PC-3 Prostate Cancer Cells. Nutrition and Cancer, 69, 952-961. https://doi.org/10.1080/01635581.2017.1340489
Matito, C., Mastorakou, F., Centelles, J.J., Torres, J.L. and Cascante, M. (2003) Antiproliferative Effect of Antioxidant Polyphenols from Grape in Murine Hepa-1c1c7. European Journal of Nutrition, 42, 43-49. https://doi.org/10.1007/s00394-003-0398-2
Veluri, R., Weir, T.L., Bais, H.P., Stermitz, F.R. and Vivanco, J.M. (2004) Phytotoxic and Antimicrobial Activities of Catechin Derivatives. Journal of Agricultural and Food Chemistry, 52, 1077-1082. https://doi.org/10.1021/jf030653+
Paulo, L., Ferreira, S., Gallardo, E., Queiroz, J.A. and Domingues, F. (2010) Antimicrobial Activity and Effects of Resveratrol on Human Pathogenic Bacteria. World Journal of Microbiology and Biotechnology, 26, 1533-1538. https://doi.org/10.1007/s11274-010-0325-7
Oliveira, V.M., et al. (2016) Quercetin and Rutin as Potential Agents Antifungal against Cryptococcus Spp. Brazilian Journal of Biology, 76, 1029-1034. https://doi.org/10.1590/1519-6984.07415
Ravn, H., Andary, C., Kovács, G. and Mølgaard, P. (1989) Caffeic Acid Esters as in Vitro Inhibitors of Plant Pathogenic Bacteria and Fungi. Biochemical Systematics and Ecology, 17, 175-184. https://doi.org/10.1016/0305-1978(89)90076-8
Ruocco, E., Donnarumma, G., Baroni, A. and Tufano, M.A. (2007) Bacterial and Viral Skin Diseases. Dermatologic Clinics, 25, 663-676. https://doi.org/10.1016/j.det.2007.06.008
Gjødsbøl, K., Christensen, J.J., Karlsmark, T., Jørgensen, B., Klein, B.M. and Krogfelt, K.A. (2006) Multiple Bacterial Species Reside in Chronic Wounds: A Longitudinal Study. International Wound Journal, 3, 225-231. https://doi.org/10.1111/j.1742-481X.2006.00159.x
Poljøak, B., Dahmane, R.G. and Godić, A. (2012) Intrinsic Skin Aging: The Role of Oxidative Stress. Acta Dermatovenerologica Alpina Pannonica et Adriatica, 21, 33-36.
Masaki, H., Atsumi, T. and Sakurai, H. (1995) Detection of Hydrogen Peroxide and Hydroxyl Radicals in Murine Skin Fibroblasts under UVB Irradiation. Biochemical and Biophysical Research Communications, 206, 474-479. https://doi.org/10.1006/bbrc.1995.1067
Kim, M.-J., et al. (2014) Anti-Photoaging Effect of Aaptamine in UVB-Irradiated Human Dermal Fibroblasts and Epidermal Keratinocytes. Journal of Asian Natural Products Research, 16, 1139-1147. https://doi.org/10.1080/10286020.2014.983092
Redza-Dutordoir, M. and Averill-Bates, D.A. (2016) Activation of Apoptosis Signalling Pathways by Reactive Oxygen Species. Biochimica et Biophysica Acta (BBA)-Molecular Cell Research, 1863, 2977-2992. https://doi.org/10.1016/j.bbamcr.2016.09.012
Jagdeo, J., et al. (2010) Dose-Dependent Antioxidant Function of Resveratrol Demonstrated via Modulation of Reactive Oxygen Species in Normal Human Skin Fibroblasts In Vitro. JDD: Journal of Drugs in Dermatology, 9, 1523-1526.
Chen, L., et al. (2018) The Antioxidant Procyanidin Reduces Reactive Oxygen Species Signaling in Macrophages and Ameliorates Experimental Colitis in Mice. Frontiers in Immunology, 8, Article 1910. https://doi.org/10.3389/fimmu.2017.01910
Kim, G.-N. and Jang, H.-D. (2009) Protective Mechanism of Quercetin and Rutin Using Glutathione Metabolism on H2O2-Induced Oxidative Stress in HepG2 Cells. Annals of the New York Academy of Sciences, 1171, 530-537. https://doi.org/10.1111/j.1749-6632.2009.04690.x
Maluf, D.F., et al. (2018) Cytoprotection of Antioxidant Biocompounds from Grape Pomace: Further Exfoliant Phytoactive Ingredients for Cosmetic Products. Cosmetics, 5, Article 46. https://doi.org/10.3390/cosmetics5030046
Azmi, N., Hashim, P., Hashim, D.M., Halimoon, N. and Majid, N.M.N. (2014) Anti-Elastase, Anti-Tyrosinase and Matrix Metalloproteinase-1 Inhibitory Activity of Earthworm Extracts as Potential New Anti-Aging Agent. Asian Pacific Journal of Tropical Biomedicine, 4, S348-S352. https://doi.org/10.12980/APJTB.4.2014C1166
Leal, C., et al. (2020) Potential Application of Grape (Vitis vinifera L.) Stem Extracts in the Cosmetic and Pharmaceutical Industries: Valorization of a By-Product. Industrial Crops and Products, 154, Article ID: 112675. https://doi.org/10.1016/j.indcrop.2020.112675
Wittenauer, J., et al. (2015) Inhibitory Effects of Polyphenols from Grape Pomace Extract on Collagenase and Elastase Activity. Fitoterapia, 101, 179-187. https://doi.org/10.1016/j.fitote.2015.01.005
Moreira, L.C., et al. (2017) In Vitro Safety and Efficacy Evaluations of a Complex Botanical Mixture of Eugenia dysenterica DC. (Myrtaceae): Prospects for Developing a New Dermocosmetic Product. Toxicology in Vitro, 45, 397-408. https://doi.org/10.1016/j.tiv.2017.04.002
Papackova, Z. and Cahova, M. (2015) Fatty Acid Signaling: The New Function of Intracellular Lipases. International Journal of Molecular Sciences, 16, 3831-3855. https://doi.org/10.3390/ijms16023831
Khan, M.H., et al. (2010) Treatment of Cellulite: Part I. Pathophysiology. Journal of the American Academy of Dermatology, 62, 361-370. https://doi.org/10.1016/j.jaad.2009.10.042
Luță, E.A., et al. (2022) The Influence of Phytosociological Cultivation and Fertilization on Polyphenolic Content of Menthae and Melissae folium and Evaluation of Antioxidant Properties through in Vitro and in Silico Methods. Plants, 11, Article 2398. https://doi.org/10.3390/plants11182398
Artem, V., Antoce, A.O., Geana, E.-I. and Ionete, R.E. (2022) Study of the Impact of Vine Cultivation Technology on the Feteasca Neagra Wine Phenolic Composition and Antioxidant Properties. Journal of Food Science and Technology, 59, 1715-1726. https://doi.org/10.1007/s13197-021-05182-6
Tseng, A. and Zhao, Y. (2012) Effect of Different Drying Methods and Storage Time on the Retention of Bioactive Compounds and Antibacterial Activity of Wine Grape Pomace (Pinot Noir and Merlot). Journal of Food Science, 77, H192-H201. https://doi.org/10.1111/j.1750-3841.2012.02840.x
Manca, M.L., et al. (2019) Phytocomplexes Extracted from Grape Seeds and Stalks Delivered in Phospholipid Vesicles Tailored for the Treatment of Skin Damages. Industrial Crops and Products, 128, 471-478. https://doi.org/10.1016/j.indcrop.2018.11.052