Up-Regulated Expression of SOD2 and HPRT1 Following Topical Photoprotection and Photorepair Skincare Formulations in A 3-Dimensional Reconstructed Human Skin Model — Oak Academic Publishing
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Up-Regulated Expression of SOD2 and HPRT1 Following Topical Photoprotection and Photorepair Skincare Formulations in A 3-Dimensional Reconstructed Human Skin Model
Clinica Tanaka Plastic, Reconstructive Surgery and Anti-Aging Center, Matsumoto, Nagano, Japan
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RATIONALE, Kyneton, Victoria, Australia
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RATIONALE, Kyneton, Victoria, Australia
1 Clinica Tanaka Plastic, Reconstructive Surgery and Anti-Aging Center, Matsumoto, Nagano, Japan
Photodamage continues to threaten human skin health despite worldwide sun awareness campaigns and the widespread use of sunscreens. To date, extensive research is lacking into the effects of sun avoidance and solar specific skincare regimens on gene expression changes and DNA repair activity. We have previously reported that photoprotection and photorepa ir formulations which minimize the harmful effects of ultraviolet, visible light and near-infrared radiation can provide photoprotection, anti-photoaging benefits and rejuvenating effects optically, clinically and genetically. To investigate gene expression changes, specifically antioxidant and DNA repair effects following the use of topical photoprotection and photorepair formulations (The Essential Six, RATIONALE, Victoria, Australia), we used epidermal keratinocytes and dermal fibroblasts derived from a 3-dimensional reconstructed human skin model, and assessed upregulation of SOD2 and HPRT1. Gene expression was assessed via the Genemarkers Standard Skin Panel and quantitative real-time PCR exploration . Tissues were inoculated with solar specific topical formulations, then co llected after 24 hours following application of photoprotection formulations and 16 hours following photorepair formulations. The quantitative real-time PCR revealed that, in comparison to the control, the genes encoding SOD2 and HPRT1 have been significantly up-regulated following usage of the photoprotection formulations, 1.86, and 1.41, respectively. SOD2 and HPRT1 were up-regulated following use of the photorepair formulations, 2.15, and 1.28, respectively. We were able to substantiate that the photo protection an d photorepair formulations upregulated genes involved in antioxidant and DNA repair mechanisms in a 3-dimensional reconstructed human skin model, suggesting a promising anti-photoaging skin regimen.
Kochevar, I.E., Pathak, M.A. and Parrish, J.A. (1999) Photophysics, Photochemistry and Photobiology. In: Freedberg, I.M., Eisen, A.Z., Wolff, K., et al., Eds., Fitzpatrick’s Dermatology in General Medicine, McGraw-Hill, New York, 220-229.
Tanaka, Y. (2012) Impact of Near-Infrared in Dermatology. World Journal of Dermatology, 1, 30-37. https://doi.org/10.5314/wjd.v1.i3.30
Tanaka, Y. and Gale, L. (2013) Beneficial Applications and Deleterious Effects of Near-Infrared from Biological and Medical Perspectives. Optics and Photonics Journal, 3, 31-39. https://doi.org/10.4236/opj.2013.34A006
Tanaka, Y. and Gale, L. (2015) Protection from Near-Infrared to Prevent Skin Damage. Optics and Photonics Journal, 5, 113-118. https://doi.org/10.4236/opj.2015.54010
Tanaka, Y. and Nakayama, J. (2016) Upregulated Epidermal Growth Factor Receptor Expression Following Near-Infrared Irradiation Simulating Solar Radiation in a Three-Dimensional Reconstructed Human Corneal Epithelial Tissue Culture Model. Clinical Interventions in Aging, 11, 1027-1033. https://doi.org/10.2147/CIA.S111530
Tanaka, Y. (2019) Long-Term Objective Assessments of Skin Rejuvenation Using Solar Protection and Solar Repair Shown through Digital Facial Surface Analysis and Three-Dimensional Volumetric Assessment. Clinical, Cosmetic and Investigational Dermatology, 12, 553-561. https://doi.org/10.2147/CCID.S218176
Tanaka, Y. (2020) Three-Dimensional Quantification of Skin Surface Displacement following Skin Rejuvenation Using Solar Protection and Solar Repair. The Journal of Clinical and Aesthetic Dermatology, 13, 47-50.
Tanaka, Y. (2023) Photoprotective Ability of Sunscreens against Ultraviolet, Visible Light and Near-Infrared Radiation. Optics and Photonics Journal, 13, 140-146. https://doi.org/10.4236/opj.2023.136012
Tanaka, Y., Parker, R. and Aganahi, A. (2023) Photoprotective Ability of Colored Iron Oxides in Tinted Sunscreens against Ultraviolet, Visible Light and Near-Infrared Radiation. Optics and Photonics Journal, 13, 199-208. https://doi.org/10.4236/opj.2023.138018
Tanaka, Y., Parker, R., Aganahi, A. and Pedroso, A. (2023) Novel Low Viscosity Zinc Oxide, Iron Oxides and Erioglaucine Sunscreen Potential to Protect from Ultraviolet, Visible Light and Near-Infrared Radiation. Optics and Photonics Journal, 13, 217-226. https://doi.org/10.4236/opj.2023.139020
Tanaka, Y., Parker, R. and Aganahi, A. (2023) Up-Regulated Expression of ICAM1, MT1A, PTGS2, LCE3D, PPARD, and GM-CSF2 following Solar Skincare Protection and Repair Strategies in a 3-Dimensional Reconstructed Human Skin Model. Clinical, Cosmetic and Investigational Dermatology, 16, 2829-2839. https://doi.org/10.2147/CCID.S428170
Widgerow, A.D. and Garruto, J.A. (2019) Gene Expression Studies Pertaining to Extracellular Matrix Integrity and Remodeling: Nuances and Pitfalls of in vitro Investigations. Journal of Drugs in Dermatology, 18, 1255-1259.
Smith, K., Nido, P., Maitra, P., Cheng, T. and Kadoya, K. (2021) Rationale and Preclinical Evaluation of a Multimodal Topical Body Skincare Product for Toning and Tightening. Journal of Drugs in Dermatology, 20, 1041-1044.
McDaniel, D.H., Dover, J.S., Wortzman, M. and Nelson, D.B. (2020) In vitro and in vivo Evaluation of a Moisture Freatment Cream Containing Three Critical Elements of Natural Skin Moisturization. Journal of Cosmetic Dermatology, 19, 1121-1128. https://doi.org/10.1111/jocd.13359
Xu, H.H., Gan, C.X., Gao, Z.Q., Huang, Y.W., Wu, S.M., Zhang, D., Wang, X. and Sheng, J. (2020) Caffeine Targets SIRT3 to Enhance SOD2 Activity in Mitochondria. Frontiers in Cell and Developmental Biology, 8, Article 822. https://doi.org/10.3389/fcell.2020.00822
Janik-Karpinska, E., Ceremuga, M., Niemcewicz, M., Synowiec, E., Sliwinski, T., Stela, M. and Bijak, M. (2023) DNA Damage Induced byT-2 Mycotoxin in Human Skin Fibroblast Cell Line-Hs68. International Journal of Molecular Sciences, 24, Article 14458. https://doi.org/10.3390/ijms241914458
Kang, T.H., Park, Y., Bader, J.S. and Friedmann, T. (2013) The Housekeeping Gene Hypoxanthine Guanine Phosphoribosyltransferase (HPRT) Regulates Multiple Developmental and Metabolic Pathways of Murine Embryonic Stem Cell Neuronal Differentiation. PLOS ONE, 8, e74967. https://doi.org/10.1371/journal.pone.0074967
Ali, S.M. and Yosipovitch, G. (2013) Skin pH: From Basic Science to Basic Skin Care. Acta Dermato-Venereologica, 93, 261-267. https://doi.org/10.2340/00015555-1531
Lukić, M., Pantelić, I. and Savić, S.D. (2021) Towards Optimal pH of the Skin and Topical Formulations: From the Current State of the Art to Tailored Products. Cosmetics, 8, Article 69. https://doi.org/10.3390/cosmetics8030069
Gupta, A., Singh, A.P., Singh, V.K., Singh, P.R., Jaiswal, J., Kumari, N., Upadhye, V., Singh, S.C. and Sinha, R.P. (2023) Natural Sun-Screening Compounds and DNA-Repair Enzymes: Photoprotection and Photoaging. Catalysts, 13, Article 745. https://doi.org/10.3390/catal13040745
Leccia, M.T., Yaar, M., Allen, N., Gleason, M. and Gilchrest, B.A. (2001) Solar Simulated Irradiation Modulates Gene Expression and Activity of Antioxidant Enzymes in Cultured Human Dermal Fibroblasts. Experimental Dermatology, 10, 272-279. https://doi.org/10.1034/j.1600-0625.2001.100407.x
Farrar, M.D., Huq, R., Mason, S., Nicolaou, A., Clarke, K.A., Dew, T.P., Williamson, G., Watson R.E.B. and Rhodes, L.E. (2018) Oral Green Tea Catechins Do Not Provide Photoprotection from Direct DNA Damage Induced by Higher Dose Solar Simulated Radiation: A Randomized Controlled Trial. Journal of the American Academy of Dermatology, 78, 414-416. https://doi.org/10.1016/j.jaad.2017.08.021
Rybchyn, M.S., De Silva, W.G.M., Sequeira, V.B., McCarthy, B.Y., Dilley, A.V., Dixon, K.M., Halliday, G.M. and Mason, R.S. (2018) Enhanced Repair of UV-Induced DNA Damage by 1, 25-Dihydroxyvitamin D3 in Skin Is Linked to Pathways That Control Cellular Energy. Journal of Investigative Dermatology, 138, 1146-1156. https://doi.org/10.1016/j.jid.2017.11.037
Khalil, C. and Shebaby, W. (2017) UVB Damage Onset and Progression 24h Post Exposure in Human-derived Skin Cells. Toxicology Reports, 4, 441-449. https://doi.org/10.1016/j.toxrep.2017.07.008
Raad, H., Serrano-Sanchez, M., Harfouche, G., Mahfouf, W., Bortolotto, D., Bergeron, V., Kasraian, Z., Dousset, L., Hosseini, M., Taieb, A. and Rezvani, H.R. (2017) NADPH Oxidase-1 Plays a Key Role in Keratinocyte Responses to UV Radiation and UVB-Induced Skin Carcinogenesis. Journal of Investigative Dermatology, 137, 1311-1321. https://doi.org/10.1016/j.jid.2016.12.027
Burnstock, G., Knight, G.E. and Greig, A.V. (2012) Purinergic Signaling in Healthy and Diseased Skin. Journal of Investigative Dermatology, 132, 526-546. https://doi.org/10.1038/jid.2011.344
Ryu, Y.S., Kang, K.A., Piao, M.J., Ahn, M.J., Yi, J.M., Hyun, Y.M., Kim, S.H., Ko, M.K., Park, C.O. and Hyun, J.W. (2019) Particulate Matter Induces Inflammatory Cytokine Production via Activation of NFκB by TLR5-NOX4-ROS Signaling in Human Skin Keratinocyte and Mouse Skin. Redox Biology, 21, Article ID: 101080. https://doi.org/10.1016/j.redox.2018.101080
Pesta, D. and Roden, M. (2017) The Janus Head of Oxidative Stress in Metabolic Diseases and During Physical Exercise. Current Diabetes Reports, 17, Article No. 41. https://doi.org/10.1007/s11892-017-0867-2
Lu, J., Zhang, H., Chen, X., Zou, Y., Li, J., Wang, L., Wu, M., Zang, J., Yu, Y., Zhuang, W., Xia, Q. and Wang, J. (2017) A Small Molecule Activator of SIRT3 Promotes Deacetylation and Activation of Manganese Superoxide Dismutase. Free Radical Biology and Medicine, 112, 287-297. https://doi.org/10.1016/j.freeradbiomed.2017.07.012
Liu, T., Ma, X., Ouyang, T., Chen, H., Xiao, Y., Huang, Y., Liu, J. and Xu, M. (2019) Efficacy of 5-Aminolevulinic Acid-Based Photodynamic Therapy against Keloid Compromised by Downregulation of SIRT1-SIRT3-SOD2-mROS Dependent Autophagy Pathway. Redox Biology, 20, 195-203. https://doi.org/10.1016/j.redox.2018.10.011