Protective and Regenerative Efficacy of a Plant Oil-Based Day and Night Cream: Investigated by a Novel Approach to Reveal the Impact of Blue Light Irradiation on Epidermal Barrier Integrity and Lipid Matrix — Oak Academic Publishing
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Protective and Regenerative Efficacy of a Plant Oil-Based Day and Night Cream: Investigated by a Novel Approach to Reveal the Impact of Blue Light Irradiation on Epidermal Barrier Integrity and Lipid Matrix
In recent years, the harmful effects of blue light (400 - 500 nm) as a component of visible light (400 - 700 nm) have increasingly gained attention of science, industry, and consumers. To date, only a few in vivo test methods for measuring the effects of blue light on the skin have been described. A direct measurement method that can detect the immediate effects of blue light on the epidermal permeability barrier (EPB) is still lacking. In this study, we present a new methodological approach that can be used to investigate both the protective and regenerative effects of cosmetic products on the EPB after blue light irradiation. In a study with 14 female volunteers, it was investigated whether the regular application of an O/W emulsion (day cream) can strengthen and protect the epidermal barrier against damaging blue light radiation of 60 J/cm 2 (protective study design) and also whether a disruption of the epidermal barrier caused by blue light radiation is restored faster and better by the regular application of another O/W emulsion (night cream) than in product-untreated skin (regenerative study design). The two O/W emulsions are different in plant oil, active ingredient composition and texture. The seven-day treatment with the day cream initially led to a significant increase in the normalized lipid lamellae length in the intercellular space, whereas the irradiation with blue light after 24 hours led to a significant decrease in the lipid lamellae length in the untreated test area, but not in the area previously treated with the product. Regarding the regenerative study design, a two-day treatment with the night cream was able to restore a blue-light-induced decrease in lipid lamellae length in the intercellular space. In summary, with the study designs presented here, the protective and regenerative effect of two cosmetic products could be demonstrated for the first time on the integrity of the EPB after blue light irradiation and the data showed that the Lipbarvis ® method is suitable for investigating the damaging effects of blue light on the EPB in vivo .
Elias, P.M. and Choi, E.H. (2005) Interactions among Stratum Corneum Defensive Functions. Experimental Dermatology , 14, 719-726. https://doi.org/10.1111/j.1600-0625.2005.00363.x
Feingold, K.R. and Elias, P.M. (2014) Role of Lipids in the Formation and Maintenance of the Cutaneous Permeability Barrier. Biochimica et Biophysica Acta ( BBA )- Molecular and Cell Biology of Lipids , 1841, 280-294. https://doi.org/10.1016/j.bbalip.2013.11.007
Proksch, E., Dähnhardt, D., S. Dähnhardt-Pfeiffer, and Fölster-Holst, R. (2016) Epidermale Barrierestörung bei Dermatosen. Der Hautarzt , 67, 907-921. https://doi.org/10.1007/s00105-016-3883-2
Meinke, M.C., Busch, L. and Lohan, S.B. (2021) Wavelength, Dose, Skin Type and Skin Model Related Radical Formation in Skin. Biophysical Reviews , 13, 1091-1100. https://doi.org/10.1007/s12551-021-00863-0
Campiche, R., Curpen, S.J., Lutchmanen‐Kolanthan, V., Gougeon, S., Cherel, M., Laurent, G., et al . (2020) Pigmentation Effects of Blue Light Irradiation on Skin and How to Protect against Them. International Journal of Cosmetic Science , 42, 399-406. https://doi.org/10.1111/ics.12637
Mahmoud, B.H., Hexsel, C.L., Hamzavi, I.H. and Lim, H.W. (2008) Effects of Visible Light on the Skin. Photochemistry and Photobiology , 84, 450-462. https://doi.org/10.1111/j.1751-1097.2007.00286.x
Sadowska, M., Narbutt, J. and Lesiak, A. (2021) Blue Light in Dermatology. Life , 11, Article 670. https://doi.org/10.3390/life11070670
Liebmann, J., Born, M. and Kolb-Bachofen, V. (2010) Blue-Light Irradiation Regulates Proliferation and Differentiation in Human Skin Cells. Journal of Investigative De r matology , 130, 259-269. https://doi.org/10.1038/jid.2009.194
Coats, J.G., Maktabi, B., Abou‐Dahech, M.S. and Baki, G. (2020) Blue Light Protection, Part I—Effects of Blue Light on the Skin. Journal of Cosmetic Dermatology , 20, 714-717. https://doi.org/10.1111/jocd.13837
Dupont, E., Gomez, J. and Bilodeau, D. (2013) Beyond UV Radiation: A Skin under Challenge. International Journal of Cosmetic Science , 35, 224-232. https://doi.org/10.1111/ics.12036
Svobodová, A. and Vostálová, J. (2010) Solar Radiation Induced Skin Damage: Review of Protective and Preventive Options. International Journal of Radiation Bio l ogy , 86, 999-1030. https://doi.org/10.3109/09553002.2010.501842
Schütz, R. (2021) Blue Light and the Skin. In: Surber, C. and Osterwalder, U., Eds., Challenges in Sun Protection , S. Karger AG, 354-373. https://doi.org/10.1159/000517644
Kumari, J., Das, K., Babaei, M., Rokni, G.R. and Goldust, M. (2023) The Impact of Blue Light and Digital Screens on the Skin. Journal of Cosmetic Dermatology , 22, 1185-1190. https://doi.org/10.1111/jocd.15576
Opländer, C., Deck, A., Volkmar, C.M., Kirsch, M., Liebmann, J., Born, M., et al . (2013) Mechanism and Biological Relevance of Blue-Light (420-453 nm)-Induced Nonenzymatic Nitric Oxide Generation from Photolabile Nitric Oxide Derivates in Human Skin in Vitro and in Vivo . Free Radical Biology and Medicine , 65, 1363-1377. https://doi.org/10.1016/j.freeradbiomed.2013.09.022
Man, M., Wakefield, J.S., Mauro, T.M. and Elias, P.M. (2021) Role of Nitric Oxide in Regulating Epidermal Permeability Barrier Function. Experimental Dermatology , 31, 290-298. https://doi.org/10.1111/exd.14470
Dungel, P., Mittermayr, R., Haindl, S., Osipov, A., Wagner, C., Redl, H., et al . (2008) Illumination with Blue Light Reactivates Respiratory Activity of Mitochondria Inhibited by Nitric Oxide, but Not by Glycerol Trinitrate. Archives of Biochemistry and Biophysics , 471, 109-115. https://doi.org/10.1016/j.abb.2008.01.009
Regazzetti, C., Sormani, L., Debayle, D., Bernerd, F., Tulic, M.K., De Donatis, G.M., et al . (2018) Melanocytes Sense Blue Light and Regulate Pigmentation through Opsin-3. Journal of Investigative Dermatology , 138, 171-178. https://doi.org/10.1016/j.jid.2017.07.833
Nakashima, Y., Ohta, S. and Wolf, A.M. (2017) Blue Light-Induced Oxidative Stress in Live Skin. Free Radical Biology and Medicine , 108, 300-310. https://doi.org/10.1016/j.freeradbiomed.2017.03.010
Ditgen, D., Segger, D., Klaucke, J., von Seebach, A., Dähnhardt, D., Dähnhardt-Pfeiffer, S., Westphal, D. and Degwert, J. (2022) Effects of Cigarette Smoke on the Skin in Comparison to UV Radiation: Parallels and Differences. Euro Cosmetics , 3, 24-30.
Lohan, S.B., Müller, R., Albrecht, S., Mink, K., Tscherch, K., Ismaeel, F., et al . (2016) Free Radicals Induced by Sunlight in Different Spectral Regions— In Vivo versus ex Vivo Study. Experimental Dermatology , 25, 380-385. https://doi.org/10.1111/exd.12987
Dang, E., Man, G., Zhang, J., Lee, D., Mauro, T.M., Elias, P.M., et al . (2020) Inducible Nitric Oxide Synthase Is Required for Epidermal Permeability Barrier Homeostasis in Mice. Experimental Dermatology , 29, 1027-1032. https://doi.org/10.1111/exd.14176
Gallala, H., Macheleidt, O., Doering, T., Schreiner, V. and Sandhoff, K. (2004) Nitric Oxide Regulates Synthesis of Gene Products Involved in Keratinocyte Differentiation and Ceramide Metabolism. European Journal of Cell Biology , 83, 667-679. https://doi.org/10.1078/0171-9335-00425
Denda, M. and Fuziwara, S. (2008) Visible Radiation Affects Epidermal Permeability Barrier Recovery: Selective Effects of Red and Blue Light. Journal of Investigative Dermatology , 128, 1335-1336. https://doi.org/10.1038/sj.jid.5701168
Coats, J.G., Maktabi, B., Abou‐Dahech, M.S. and Baki, G. (2020) Blue Light Protection, Part II—Ingredients and Performance Testing Methods. Journal of Cosmetic Dermatology , 20, 718-723. https://doi.org/10.1111/jocd.13854
Tsuchida, K. and Sakiyama, N. (2022) Blue Light-Induced Lipid Oxidation and the Antioxidant Property of Hypotaurine: Evaluation via Measuring Ultraweak Photon Emission. Photochemical & Photobiological Sciences , 22, 345-356. https://doi.org/10.1007/s43630-022-00319-8
Pourang, A., Tisack, A., Ezekwe, N., Torres, A.E., Kohli, I., Hamzavi, I.H., et al . (2021) Effects of Visible Light on Mechanisms of Skin Photoaging. Photodermato l ogy , Photoimmunology & Photomedicine , 38, 191-196. https://doi.org/10.1111/phpp.12736
Daehnhardt-Pfeiffer, S., Surber, C., Wilhelm, K.-P., Daehnhardt, D., Springmann, G., Boettcher, M., et al . (2012) Noninvasive Stratum Corneum Sampling and Electron Microscopical Examination of Skin Barrier Integrity: Pilot Study with a Topical Glycerin Formulation for Atopic Dermatitis. Skin Pharmacology and Physiology , 25, 155-161. https://doi.org/10.1159/000336789
Dähnhardt, D., Surber, C. and Dähnhardt-Pfeiffer, S. (2018) Influence of Topical Formulations: Lipid Lamella Organization and Lipid Composition of Stratum Corneum as a Surrogate Marker for Barrier Integrity. In: Surber, C., Maibach, H. and Abels, C., Eds., pH of the Skin : Issues and Challenges , S. Karger AG, 166-172. https://doi.org/10.1159/000489530
Dähnhardt, D., Dähnhardt-Pfeiffer, S., Simon, I., Ditgen, D., Holland, I., Segger, D., et al . (2023) Protective and Regenerative Anti-Pollution Efficacy of a Plant Oil-Based Day and Night Cream: Investigated by a Novel Approach to Reveal the Impact of Pollutants on Epidermal Barrier Integrity and Lipid Matrix. Journal of Cosmetics , Dermatological Sciences and Applications , 13, 302-321. https://doi.org/10.4236/jcdsa.2023.134024
Thiele, J.J., Schroeter, C., Hsieh, S.N., Podda, M. and Packer, L. (2000) The Antioxidant Network of the Stratum Corneum. In: Thiele, J. and Elsner, P., Eds., Oxi dants and Antioxidants in Cutaneous Biology , S. Karger AG, 26-42. https://doi.org/10.1159/000060651
Darvin, M., Zastrow, L., Sterry, W. and Lademann, J. (2006) Effect of Supplemented and Topically Applied Antioxidant Substances on Human Tissue. Skin Pharmaco l ogy and Physiology , 19, 238-247. https://doi.org/10.1159/000093979
Lademann, J., Schanzer, S., Meinke, M., Sterry, W. and Darvin, M.E. (2011) Interaction between Carotenoids and Free Radicals in Human Skin. Skin Pharmacology and Physiology , 24, 238-244. https://doi.org/10.1159/000326074
Vandersee, S., Beyer, M., Lademann, J. and Darvin, M.E. (2015) Blue-Violet Light Irradiation Dose Dependently Decreases Carotenoids in Human Skin, Which Indicates the Generation of Free Radicals. Oxidative Medicine and Cellular Longevity , 2015, Article ID: 579675. https://doi.org/10.1155/2015/579675
Schulte to Brinke, A., Mehlich, A., Doberenz, C. and Janssens-Böcker, C. (2021) Acidification of the Skin and Maintenance of the Physiological Skin pH Value by Buffered Skin Care Products Formulated around PH 4. Journal of Cosmetics , De r matological Sciences and Applications , 11, 44-57. https://doi.org/10.4236/jcdsa.2021.111005