<i>In Vitro</i> Evaluation of Gamma Irradiation on a Gel Formulation of Cratylia Mollis: Rheological Proporties and Microbiological Control — Oak Academic Publishing
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<i>In Vitro</i> Evaluation of Gamma Irradiation on a Gel Formulation of Cratylia Mollis: Rheological Proporties and Microbiological Control
Laboratory of Immunopathology Keizo Asami (LIKA), Federal University of Pernambuco, Recife, Brazil
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Nuclear Engineering Institute, Laboratory of Nanoradiopharmaceuticals, Rio de Janeiro, Brazil
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Biochemistry Department, Federal University of Pernambuco, Recife, Brazil
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Pharmacy Department, University of Sao Paulo, Sao Paulo, Brazil
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Pharmacy Department, University of Sao Paulo, Sao Paulo, Brazil
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School of Chemistry, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil
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Biochemistry Department, Federal University of Pernambuco, Recife, Brazil
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Biochemistry Department, Federal University of Pernambuco, Recife, Brazil
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Departament of Morphology and Animal Physiology, Federal Rural University of Pernambuco, Recife, Brazil.
1 Laboratory of Immunopathology Keizo Asami (LIKA), Federal University of Pernambuco, Recife, Brazil
2 Nuclear Engineering Institute, Laboratory of Nanoradiopharmaceuticals, Rio de Janeiro, Brazil
3 Biochemistry Department, Federal University of Pernambuco, Recife, Brazil
4 Pharmacy Department, University of Sao Paulo, Sao Paulo, Brazil
5 Pharmacy Department, University of Sao Paulo, Sao Paulo, Brazil
6 School of Chemistry, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil
7 Biochemistry Department, Federal University of Pernambuco, Recife, Brazil
8 Biochemistry Department, Federal University of Pernambuco, Recife, Brazil
9 Departament of Morphology and Animal Physiology, Federal Rural University of Pernambuco, Recife, Brazil.
Lectin Cramoll-1,4, obtained from Cratylia mollis seeds (beans camaratu) was structurally characterized, biologically and pharmacologically, but its use as a biopharmaceutical is not well documented. The objective of this study is to propose a biopharmaceutical formulation lectin Cramoll-1,4, test their hemagglutinating properties in vitro as well as the use of gamma radiation as a continuous process of decontamination formulation. It was made of the extraction and purification Cramoll-1,4, was developed a gel formulation using Carbopol ? as a vehicle, at concentrations of 50, 100, 200, 300 and 600 μg was irradiated with 60 Co gamma rays in a dose of 7.549 kGy·h –1 . The proposed formulation at a concentration of 300 μg produced an increase in the hemagglutinating units Cramoll-1,4 due to the synergistic effect caused by gamma radiation. Considering the diverse use of lectins, specific molecular and structural factors, as well as changes resulting from its formulation, concentration, irradiation and route of administration is of utmost importance to continue the studies in vitro , for subsequent application in vivo to characterize the physiological and molecular processes involved in the response and cellular effects.
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