TiO<sub>2</sub> Nanoparticles Induced Genotoxicity in Cultured Cells Using Atmospheric Scanning Electron Microscopy (ASEM)
- 1 Division of Morphological and Biomolecular Research, Graduate School of Medicine, Nippon Medical School, Tokyo, Japan
- 2 R & D business Support Department Group 1 DATUM Solution business Operations, JEOL Ltd., Tokyo, Japan
- 3 R & D business Support Department Group 1 DATUM Solution business Operations, JEOL Ltd., Tokyo, Japan
- 4 Department of Der- matology, Queen’s Square Medical Center, Yokohama, Japan
Abstract
Nano-sized titanium oxide nanoparticles (TiO 2 NPs) are widely used as a dye in food and cosmetics. TiO 2 NPs are known to induce DNA damage when incorporated into cells. However, no bioassay is currently available to easily determine the cell incorporation of TiO 2 NPs or related DNA damage, and to date, few studies have examined the different degrees of incorporation into cells according to the size of the TiO 2 NPs particles and the presence or absence of cell specificity regarding DNA damage. This present study was therefore designed to examine COS7 cells that had incorporated TiO 2 NPs using atmospheric scanning electron microscopy (ASEM). The results indicated that absorption of TiO 2 NPs into cells and nuclear abnormalities had occurred. ASEM is a rapid and simple technique that enables the observation of samples immediately after fixation with glutaraldehyde and staining with phosphotungstic acid, and this method was suggested to be useful in screening for DNA damage.
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