Isolation of Anti Ageing Molecules Secreted by Human Gingival Stem Cells. Protection of Collagen and Elastic Networks

Abstract

Skin aging results in an imbalance between synthesis and degradation of the extracellular matrix. Overproduction of degradative enzymes (MMPs) and oxygen free radicals during chronological and photo-induced aging leads a degradation of the network and elastic skin collagen. Our previous work demonstrated that a culture supernatant of gingival stem cells had anti-aging activities in vitro , ex vivo and in vivo in humans. However, this culture medium is very complex and currently not responding to the European cosmetics regulation. After the analysis of the culture medium we have isolated 4 molecules interesting in terms of skin anti-aging activity: TIMP1, Selenium, Folic Acid and Glycin (TSAG). After the study of the most efficiency concentration of each molecule, the composition of TSAG is tested on irradiated UVA human dermal fibroblast and human skin. TSAG preserves the elastin and collagen network and inhibits the MMP1, MMP3 and MMP9 activities. The advantage of this mimetic solution of stem cells is to be stable, easily reproducible and non-human origin. The outlook for cosmetology seems interesting.