Antioxidant, Collagen Synthesis Activity <i>in Vitro</i> and Clinical Test on Anti-Wrinkle Activity of Formulated Cream Containing <i>Veronica officinalis</i> Extract

Abstract

In this study, our objective was to evaluate the antioxidant, cytotoxicity and collagen synthesis activity in vitro and also to test the anti-wrinkle effect of formulated cream containing Veronica officinalis extract in vivo. Antioxidant evaluation was based on the scavenging activity of free radicles (DPPH) and procollagen type 1 protein (P1P) synthesis test was performed in fibroblast cell. Clinical anti-wrinkle activity was performed on female subjects in placebo-controlled trail. Verbascoside (an isolated compound) showed higher (IC 50 value of 36.24 ± 1.81 μg/ml) free radicle inhibition activity but weaker collagen synthesis activity. The ethanolic extract showed good inhibition to DPPH free radicals and also showed a significant effect in collagen synthesis activity without cytotoxicity. In the i n vivo study, treatment with the formulated cream (Scoti-Speedwell) for 56 days significantly reduced the percentage of wrinkle area and length with 18.0% and 16.05%, respectively. Overall, Veronica officinalis extract containing product (Scoti-Speedwell ^TM ) can be regarded as a potent anti-wrinkle agent in human skin.