In <i>Vitro</i> Effects of the Phytocomplex TrichoTech<sup>TM</sup> on Human Fibroblasts: Proliferative Potential and Effects on Gene Expression of FGF-7 and FGF-10 — Oak Academic Publishing
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In <i>Vitro</i> Effects of the Phytocomplex TrichoTech<sup>TM</sup> on Human Fibroblasts: Proliferative Potential and Effects on Gene Expression of FGF-7 and FGF-10
Instituto de Osmologia e óleos Essenciais, Minas Gerais, Brazil
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Instituto de Osmologia e óleos Essenciais, Minas Gerais, Brazil
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Instituto de Osmologia e óleos Essenciais, Minas Gerais, Brazil
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Centro Interdisciplinar de Investigação Bioquímica, Universidade de Mogi das Cruzes, São Paulo, Brazil
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Grupo de Fitocomplexos e Sinalização Celular, Escola de Ciência da Saúde, Universidade Anhembi Morumbi, São Paulo, Brazil
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Centro Interdisciplinar de Investigação Bioquímica, Universidade de Mogi das Cruzes, São Paulo, Brazil
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Instituto de Osmologia e óleos Essenciais, Minas Gerais, Brazil
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Departamento de Farmacologia, Universidade Federal de São Paulo, São Paulo, Brazil
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Instituto de Osmologia e óleos Essenciais, Minas Gerais, Brazil
1 Instituto de Osmologia e óleos Essenciais, Minas Gerais, Brazil
2 Instituto de Osmologia e óleos Essenciais, Minas Gerais, Brazil
3 Instituto de Osmologia e óleos Essenciais, Minas Gerais, Brazil
4 Centro Interdisciplinar de Investigação Bioquímica, Universidade de Mogi das Cruzes, São Paulo, Brazil
5 Grupo de Fitocomplexos e Sinalização Celular, Escola de Ciência da Saúde, Universidade Anhembi Morumbi, São Paulo, Brazil
6 Centro Interdisciplinar de Investigação Bioquímica, Universidade de Mogi das Cruzes, São Paulo, Brazil
7 Instituto de Osmologia e óleos Essenciais, Minas Gerais, Brazil
8 Departamento de Farmacologia, Universidade Federal de São Paulo, São Paulo, Brazil
9 Instituto de Osmologia e óleos Essenciais, Minas Gerais, Brazil
The human hair follicle, a mini-organ formed with neuroectodermal-meso-dermal interaction, is a complex structure, in the active steady state (anagen) the dermal papilla can be considered as a ball of extracellular matrix, surrounding specialized fibroblasts. The cross-talk of dermal papilla with neighbouring matrix cells results in the maintenance of hair fibre production. This study aimed to investigate the proliferative potential of the compound Trichotech TM , a phytocomplex obtained from a mixture of essential oils, on cultured human fibroblasts and its ability to modulate the gene expression of FGF-7 and FGF-10. Trichotech TM was shown to enhance fibroblasts proliferation in concentrations of 0.5% to 2.0%, and also increase the percentage of cells in the S/G2/M phases of the cell cycle. Trichotech TM at both 1.0% and 2.0% induced a statistically significant effect on wound healing assay compared to the untreated control. We examined the interaction between cell survival (PI3K/Akt) and mitogenic (Ras/MAPK) signal transduction pathways after Trichotech TM treatment (1.0% and 2.0%) on the fibroblast cell line. Trichotech TM caused phosphorylation of ERK1/2, as well as greater phosphorylation of MEK in comparison with both the untreated control and ERK1/2. PI3K and AKT, however, were not shown to be significantly more phosphorylated following TrichotechTM exposure. To verify the relative expression of mRNA for FGF-7 and FGF-10 genes, a real-time polymerase chain reaction (qPCR) protocol was used. Results show the increase in mRNA expression by fibroblasts after treatment with Trichotech TM . In both concentrations tested, Trichotech TM was found to increase the expression of FGF-7 and FGF-10. Sirius red staining allows for rapid assessment of collagen content, it showed a significant increase in collagen content in treated fibroblasts. Further investigation concerning Trichotech TM could be helpful towards the development of new bioactive phytocomplexes for dermatological and trichological use.
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