Effects of Chemotherapy on Peripheral Blood NK Cell Receptor NKG2D and Related Immune Cytokines in Patients with Non-Small Cell Lung Cancer
- 1 Department of Oncology, the Affiliated Hospital of Chengde Medical University, Chengde, China
- 2 Department of Urology, the Affiliated Hospital of Chengde Medical University, Chengde, China
- 3 Department of Oncology, the Affiliated Hospital of Chengde Medical University, Chengde, China
- 4 Department of Oncology, the Affiliated Hospital of Chengde Medical University, Chengde, China
- 5 Department of Oncology, the Affiliated Hospital of Chengde Medical University, Chengde, China
- 6 Department of Oncology, the Affiliated Hospital of Chengde Medical University, Chengde, China
- 7 Department of Oncology, the Affiliated Hospital of Chengde Medical University, Chengde, China
- 8 Department of Oncology, the Affiliated Hospital of Chengde Medical University, Chengde, China
- 9 Department of Oncology, the Affiliated Hospital of Chengde Medical University, Chengde, China
- 10 Department of Oncology, the Affiliated Hospital of Chengde Medical University, Chengde, China
Abstract
Objective: To analyze the effect of chemotherapy on peripheral blood NK cell receptor NKG2D and related immune cytokines (IL-12 , IL-15 , IL-18) in patients with non-small cell lung cancer (NSCLC). Methods: A total of 48 patients with NSCLC who visited the Oncology Department of the Affiliated Hospital of Chengde Medical College from September 2018 to September 2019 were selected as the study subjects. Changes in the expression levels of NKG2D, IL-12, IL-15 and IL-18 in peripheral blood of patients at different time points (before chemotherapy, after the first chemotherapy and after the second chemotherapy) were analyzed to investigate the correlation between NKG2D and IL-12, IL-15 and IL-18 in peripheral blood at each time point. Results: The expression levels of NKG2D, IL-15, and IL-18 in the peripheral blood of the patient before chemotherapy, after the first chemotherapy, and after the second chemotherapy gradually decreased. After the first chemotherapy and the second chemotherapy, the peripheral blood IL-12 was significantly lower than before chemotherapy, and IL-12 in peripheral blood after the second chemotherapy was slightly increased compared with that after the first chemotherapy. The comparison of each factor at different time points was statistically significant (all P < 0.05). Pearson correlation analysis showed that after the first chemotherapy, NKG2D in peripheral blood was positively correlated with IL-18 (r = 0.342, P = 0.031); after the second chemotherapy, NKG2D in peripheral blood was positively correlated with IL-18 (r = 0.411, P = 0.023), negatively correlated with IL-15 (r = -0.451, P = 0.001). Conclusion: There was no significant change in the number of NK cells in the peripheral blood of NSCLC patients after chemotherapy, while NKG2D and related immune cytokines decreased, which may be one of the mechanisms for the suppression of immune function in patients, and this provides a potential target for immunotherapy in patients.
- Yang, G.J. and Wang, Y. (2020) Current Status and Perspectives of Treatment of Non-Small Cell Lung Cancer with Insertional Mutations in Exon 20 of the Epidermal Growth Factor Receptor Gene. Chinese Journal of Oncology, 42, 22-29.
- Gou, S.-L., Yuan, C., Bo, Y.-J., et al. (2020) Detection and Clinical Significance of Peripheral Blood Tumor Cells in Advanced Non-Small Cell Lung Cancer. Chinese Journal of Gerontology, 40, 730-733.
- Lu, Z.L. (2020) Research Progress of Immune Checkpoint Inhibitors in the Treatment of Non-Small Cell Lung Cancer. Journal of Doubtful Diseases, 19, 311-316.
- Xu Z. (2019) Study on the Effect of Chemotherapy on NK Cell Receptor NKG2D and Related Immune Cytokines in Peripheral Blood of Patients with Non-Small Cell Lung Cancer. Chengde Medical College, Chengde, 1-37.
- Wang, C.H., He, Y.F. and Hu, B. (2012) Study on the Anti-Tumor Mechanism of Cytokine-Induced Killer Cells and Its Application. International Journal of Oncology, 39, 348-351.
- Li, F. (2017) Reflections Arising from Reading the AJCC 8th Edition Lung Cancer Staging System. Chinese Journal of Surgery, 55, 346-350.
- Song, H.S. (2018) Co-Culture with NK-92MI Cells Enhanced the Anti-Cancer Effect of Bee Venom on NSCLC Cells by Inactivation of NF-κB. Journal of Acupuncture and Meridian Studies, 11, 178-179. https://doi.org/10.1016/j.jams.2018.08.032
- Zheng, L. and Chen, J. (2021) Effect of Combination Treatment Regimen on Prognosis and Immune Function in Patients with Advanced Non-Small Cell Lung Cancer. Journal of Practical Clinical Medicine, 25, 52-56.
- Siegel, R.L., Miller, K.D. and Jemal, A. (2016) Cancer Statistics, 2016. CA: A Cancer Journal for Clinicians, 66, 7-30. https://doi.org/10.3322/caac.21332
- Chitadze, G., Bhat, J., Lettau, M., et al. (2013) Generation of Soluble NKG2D Ligands: Proteolytic Cleavage, Exosome Secretion and Functional Implications. Scandinavian Journal of Immunology, 78, 120-129. https://doi.org/10.1111/sji.12072
- Nie, Y., Du, L., Mou, Y., et al. (2013) Effect of Low Frequency Magnetic Fields on Melanoma: Tumor Inhibition and Immune Modulation. BMC Cancer, 13, Article No. 582. https://doi.org/10.1186/1471-2407-13-582
- Yuan, L.F., Su, W., Wang, Y.F., et al. (2011) Study on the Expression of NKG2A and NKG2D in Peripheral Blood of Lung Cancer Patients and Their Clinical Significance. Chinese Journal of Cancer Control, 18, 1924-1928.