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REGγ Mediated Regulation of p21<sup>Waf/Cip1</sup>, p16<sup>INK4a</sup> and p14<sup>ARF</sup>/p19<sup>ARF</sup> <i>in Vivo</i>
The Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences, East China Normal University, Shanghai, China
Department of Pathology, The Second Chengdu Municipal Hospital, Chengdu, China
The Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences, East China Normal University, Shanghai, China
The Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences, East China Normal University, Shanghai, China
The Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences, East China Normal University, Shanghai, China
The Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences, East China Normal University, Shanghai, China
The Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences, East China Normal University, Shanghai, China
The Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences, East China Normal University, Shanghai, China
The Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences, East China Normal University, Shanghai, China
The Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences, East China Normal University, Shanghai, China
The Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences, East China Normal University, Shanghai, China
The Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences, East China Normal University, Shanghai, China
The Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences, East China Normal University, Shanghai, China
Department of Orthopaedic Oncology, Changzheng Hospital, The Second Military Medical University, Shanghai, China
The Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences, East China Normal University, Shanghai, China
The Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences, East China Normal University, Shanghai, China
- 1 The Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences, East China Normal University, Shanghai, China
- 2 Department of Pathology, The Second Chengdu Municipal Hospital, Chengdu, China
- 3 The Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences, East China Normal University, Shanghai, China
- 4 The Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences, East China Normal University, Shanghai, China
- 5 The Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences, East China Normal University, Shanghai, China
- 6 The Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences, East China Normal University, Shanghai, China
- 7 The Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences, East China Normal University, Shanghai, China
- 8 The Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences, East China Normal University, Shanghai, China
- 9 The Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences, East China Normal University, Shanghai, China
- 10 The Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences, East China Normal University, Shanghai, China
- 11 The Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences, East China Normal University, Shanghai, China
- 12 The Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences, East China Normal University, Shanghai, China
- 13 The Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences, East China Normal University, Shanghai, China
- 14 Department of Orthopaedic Oncology, Changzheng Hospital, The Second Military Medical University, Shanghai, China
- 15 The Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences, East China Normal University, Shanghai, China
- 16 The Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences, East China Normal University, Shanghai, China
Journal of Cancer Therapy·Volume 04 (2013)·Pages 933–938·Published 28 June 2013·DOI10.4236/jct.2013.45104
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Abstract
p 21 Waf/Cip1 , p16 INK4a and p14 ARF (p19 ARF in mice) have been demonstrated to be degraded by REG γ -proteasome pathway in an ATP- and ubiquitin-independent manner in vitro . However, the in vivo roles of REG γ mediated-degradation of p21 Waf/Cip1 , p16 INK4a and p14 ARF remain unclear. In this study, we showed enhanced expression of p21 Waf/Cip1 , p16 INK4a and p19 ARF in multiple tissues from REG g –/– mice compared to REG g +/+ mice. Furthermore, we examined the expression of p21 Waf/Cip1 , p16 INK4a and p14 ARF in different cancer tissues and observed that the REG γ protein levels were highly expressed in different human cancers while the level of p21 Waf/Cip1 , p16 INK4a and p14 ARF appears to be inversely corre lated. These results demonstrate that REG γ may exert its function in physiological and pathological conditions through degradation of p21 Waf/Cip1 , p16 INK4a and p14 ARF in vivo .
KeywordsREGγp21<sup>Waf/Cip1</sup>p16<sup>INK4a</sup>p14<sup>ARF</sup>p19<sup>ARF</sup>Cancer
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