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Colon Adenocarcinoma Diagnosis in Human Samples by Multicontrast Nonlinear Optical Microscopy of Hematoxylin and Eosin Stained Histological Sections
Microscopy Laboratory Applied to Molecular and Cellular Studies, School of Engineering, National University of Entre Ríos (UNER), Oro Verde, Argentina
Microscopy Laboratory Applied to Molecular and Cellular Studies, School of Engineering, National University of Entre Ríos (UNER), Oro Verde, Argentina
INFABiC-National Institute of Science and Technology on Photonics Applied to Cell Biology, Campinas, Brazil
Microscopy Laboratory Applied to Molecular and Cellular Studies, School of Engineering, National University of Entre Ríos (UNER), Oro Verde, Argentina
Microscopy Laboratory Applied to Molecular and Cellular Studies, School of Engineering, National University of Entre Ríos (UNER), Oro Verde, Argentina
INFABiC-National Institute of Science and Technology on Photonics Applied to Cell Biology, Campinas, Brazil
Department of Structural and Functional Biology, Institute of Biology, State University of Campinas (UNICAMP), Campinas, Brazil
Microscopy Laboratory Applied to Molecular and Cellular Studies, School of Engineering, National University of Entre Ríos (UNER), Oro Verde, Argentina
Research and Transfer Center of Entre Ríos (CITER) CONICET-UNER, Oro Verde, Argentina
Biophotonic Group, Optics and Photonics Research Center (CEPOF), Institute of Physics “Gleb Wataghin”, State University of Campinas (UNICAMP), Campinas, Brazil
- 1 Microscopy Laboratory Applied to Molecular and Cellular Studies, School of Engineering, National University of Entre Ríos (UNER), Oro Verde, Argentina
- 2 Microscopy Laboratory Applied to Molecular and Cellular Studies, School of Engineering, National University of Entre Ríos (UNER), Oro Verde, Argentina
- 3 INFABiC-National Institute of Science and Technology on Photonics Applied to Cell Biology, Campinas, Brazil
- 4 Microscopy Laboratory Applied to Molecular and Cellular Studies, School of Engineering, National University of Entre Ríos (UNER), Oro Verde, Argentina
- 5 Microscopy Laboratory Applied to Molecular and Cellular Studies, School of Engineering, National University of Entre Ríos (UNER), Oro Verde, Argentina
- 6 INFABiC-National Institute of Science and Technology on Photonics Applied to Cell Biology, Campinas, Brazil
- 7 Department of Structural and Functional Biology, Institute of Biology, State University of Campinas (UNICAMP), Campinas, Brazil
- 8 Microscopy Laboratory Applied to Molecular and Cellular Studies, School of Engineering, National University of Entre Ríos (UNER), Oro Verde, Argentina
- 9 Research and Transfer Center of Entre Ríos (CITER) CONICET-UNER, Oro Verde, Argentina
- 10 Biophotonic Group, Optics and Photonics Research Center (CEPOF), Institute of Physics “Gleb Wataghin”, State University of Campinas (UNICAMP), Campinas, Brazil
Journal of Cancer Therapy·Volume 05 (2014)·Pages 1259–1269·Published 6 November 2014·DOI10.4236/jct.2014.513127
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Abstract
Combined multimodal nonlinear optical (NLO) microscopies were used to detect and quantify morphological changes associated with stroma and epithelial transformation in colon cancer. Our findings provide complementary information about tissue microstructure, displaying distinctive patterns between normal and malignant human colon. Additionally, we have demonstrated the usefulness of using fixed tissues for the disease diagnostic and prognostic. The present work provides a framework for using NLO techniques as a clinical diagnostic tool for human colon cancer. NLO metrics could be applied to other disorders, which are characterized by abnormal cell proliferation and collagen assembly.
KeywordsNonlinear MicroscopyNonlinear MulticontrastHistological SectionsHuman Colon Cancer
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