<i>Dunaliella salina</i> and <i>Haloferax volcanii</i> Synergistically Attenuate Skin Cancer <i>in Vitro</i> — Oak Academic Publishing
Research ArticleOpen AccessGoogle Scholar indexed
<i>Dunaliella salina</i> and <i>Haloferax volcanii</i> Synergistically Attenuate Skin Cancer <i>in Vitro</i>
The Skin Research Institute, The Dead-Sea & Arava Science Center, Masada, Israel
,
The Skin Research Institute, The Dead-Sea & Arava Science Center, Masada, Israel
,
Clinic Lenom Ltd., Rishon Lezion, Israel
,
Department of Microbiology, Immunology and Genetics, Center for Emerging and Tropical Diseases and AIDS, Ben Gurion University of the Negev, Beer Sheba, Israel
,
The Skin Research Institute, The Dead-Sea & Arava Science Center, Masada, Israel
1 The Skin Research Institute, The Dead-Sea & Arava Science Center, Masada, Israel
2 The Skin Research Institute, The Dead-Sea & Arava Science Center, Masada, Israel
3 Clinic Lenom Ltd., Rishon Lezion, Israel
4 Department of Microbiology, Immunology and Genetics, Center for Emerging and Tropical Diseases and AIDS, Ben Gurion University of the Negev, Beer Sheba, Israel
5 The Skin Research Institute, The Dead-Sea & Arava Science Center, Masada, Israel
Skin cancer, including both melanoma and non-melanoma, is the most common type of malignancy, which causes substantial morbidities and mortalities. Although the significant increase in the understanding of skin cancer formation and the development of novel personalized drug regimens ha ve occurred, new treatment options are always of need. The use of natural compounds to alleviate the symptoms or even to prevent and treat cancer has long been proposed. Specifically, the use of marine-based organisms as a source for cancer cure and remedy is being evaluated extensively. The objective of the current study was to assess the ability of the green microalgae Dunaliella salina , the Dead-Sea-derived Haloferax volcanii , and its combinations to treat skin cancer in vitro . The results demonstrate the Dunaliella and Haloferax can reduce sarcoma and basal cell carcinoma cellular growth. Importantly, their combination act s synergistically in a caspase-3 independent manner. Moreover, a synergistic action was found when evaluated sarcoma cell invasion rate, which was completely blocked at pharmacological relevant amounts of the compounds. Collectively, the results demonstrate that the combination of Haloferax volcanii and Dunaliella salina can be used as a new treatment for skin cancer. The specific mechanism of action and further in vivo validation studies are of need.
Seebode, C., Lehmann, J. and Emmert, S. (2016) Photocarcinogenesis and Skin Cancer Prevention Strategies. Anticancer Research, 36, 1371-1378.
Martens, M.C., Seebode, C., Lehmann, J. and Emmert, S. (2018) Photocarcinogenesis and Skin Cancer Prevention Strategies: An Update. Anticancer Research, 38, 1153-1158. https://doi.org/10.21873/anticanres.12334
Armstrong, B.K. and Kricker, A. (2001) The Epidemiology of UV Induced Skin Cancer. Journal of Photochemistry and Photobiology B: Biology, 63, 8-18. https://doi.org/10.1016/S1011-1344(01)00198-1
Kahremany, S., Babaev, I., Gvirtz, R., Ogen-Stern, N., Azoulay-Ginsburg, S., Senderowitz, H., Cohen, G. and Gruzman, A. (2019) Nrf2 Activation by SK-119 Attenuates Oxidative Stress, UVB, and LPS-Induced Damage. Skin Pharmacology and Physiology, 32, 173-181. https://doi.org/10.1159/000499432
Wineman, E., Douglas, I., Wineman, V., Sharova, K., Jaspars, M., Meshner, S., Bentwich, Z., Cohen, G. and Shtevi, A. (2015) Commiphora gileadensis Sap Extract Induces Cell Cycle-Dependent Death in Immortalized Keratinocytes and Human Dermoid Carcinoma Cells. Journal of Herbal Medicine, 5, 199-206. https://doi.org/10.1016/j.hermed.2015.08.001
Apalla, Z., Nashan, D., Weller, R.B. and Castellsagué, X. (2017) Skin Cancer: Epidemiology, Disease Burden, Pathophysiology, Diagnosis, and Therapeutic Approaches. Dermatology and Therapy, 7, 5-19. https://doi.org/10.1007/s13555-016-0165-y
Kohlmeyer, J., Steimle-Grauer, S.A. and Hein, R. (2017) Cutaneous Sarcomas. JDDG: Journal der Deutschen Dermatologischen Gesellschaft, 15, 630-648. https://doi.org/10.1111/ddg.13249
Mentzel, T. (2011) Sarcomas of the Skin in the Elderly. Clinics in Dermatology, 29, 80-90. https://doi.org/10.1016/j.clindermatol.2010.07.011
Wargovich, M.J., Woods, C., Hollis, D.M. and Zander, M.E. (2018) Herbals, Cancer Prevention and Health. The Journal of Nutrition, 131, 3034S-3036S. https://doi.org/10.1093/jn/131.11.3034S
Yin, S.Y., Wei, W.C., Jian, F.Y. and Yang, N.S. (2013) Therapeutic Applications of Herbal Medicines for Cancer Patients. Evidence-Based Complementary and Alternative Medicine, 2013, Article ID: 302426. https://doi.org/10.1155/2013/302426
Mishra, A., Kavita, K. and Jha, B. (2011) Characterization of Extracellular Polymeric Substances Produced by Micro-Algae Dunaliella salina. Carbohydrate Polymers, 83, 852-857. https://doi.org/10.1016/j.carbpol.2010.08.067
Oren, A. (2005) A Hundred Years of Dunaliella Research: 1905-2005. Saline Systems, 1, 2. https://doi.org/10.1186/1746-1448-1-2
Francavilla, M., Colaianna, M., Zotti, M., Morgese, M.G., Trotta, P., Tucci, P., Schiavone, S., Cuomo, V. and Trabace, L. (2012) Extraction, Characterization and in Vivo Neuromodulatory Activity of Phytosterols from Microalga Dunaliella Tertiolecta. Current Medicinal Chemistry, 19, 3058-3067. https://doi.org/10.2174/092986712800672021
Jafari, S., Mobasher, M.A., Najafipour, S., Ghasemi, Y., Mohkam, M., Ebrahimi, M.A. and Mobasher, N. (2018) Antibacterial Potential of Chlorella vulgaris and Dunaliella salina Extracts against Streptococcus mutans. Jundishapur Journal of Natural Pharmaceutical Products, 13, e13226. https://doi.org/10.5812/jjnpp.13226
El-Baz, F., Abdel Jaleel, G., Saleh, D. and Hussein, R. (2018) Protective and Therapeutic Potentials of Dunaliella salina on Aging-Associated Cardiac Dysfunction in Rats. Asian Pacific Journal of Tropical Biomedicine, 8, 403-410. https://doi.org/10.4103/2221-1691.239428
Srinivasan, R., Chaitanyakumar, A., Mageswari, A., Gomathi, A., Pavan, J.G.S., Kumar, M., Jayasindu, G., Bharath, J.S. and Shravan, K.M. (2017) Gothandam, Oral Administration of Lyophilized Dunaliella salina, a Carotenoid-Rich Marine Alga, Reduces Tumor Progression in Mammary Cancer Induced Rats. Food & Function, 8, 4517-4527. https://doi.org/10.1039/C7FO01328K
Pohlschroder, M. and Schulze, S. (2019) Haloferax volcanii. Trends in Microbiology, 27, 86-87. https://doi.org/10.1016/j.tim.2018.10.004
Venugopal, V. (2008) Marine Products for Healthcare: Functional and Bioactive Nutraceutical Compounds from the Ocean. CRC Press, Boca Raton, FL. https://doi.org/10.1201/9781420052640
Sathasivam, R., Radhakrishnan, R., Hashem, A. and Abd-Allah, E.F. (2019) Microalgae Metabolites: A Rich Source for Food and Medicine. Saudi Journal of Biological Sciences, 26, 709-722. https://doi.org/10.1016/j.sjbs.2017.11.003
El-Baz, F.K., Abdo, S.M. and Hussein, A.M.S. (2017) Microalgae Dunaliella salina for Use as Food Supplement to Improve Pasta Quality. International Journal of Pharmaceutical Sciences Review and Research, 46, 45-51.
Martínez Andrade, K.A., Lauritano, C., Romano, G. and Ianora, A. (2018) Marine Microalgae with Anti-Cancer Properties. Marine Drugs, 16, 165. https://doi.org/10.3390/md16050165
Pasquet, V., Morisset, P., Ihammouine, S., Chepied, A., Aumailley, L., Berard, J.B., Serive, B., Kaas, R., Lanneluc, I., Thiery, V., Lafferriere, M., Piot, J.M., Patrice, T., Cadoret, J.P. and Picot, L. (2011) Antiproliferative Activity of Violaxanthin Isolated from Bioguided Fractionation of Dunaliella tertiolecta Extracts. Marine Drugs, 9, 819-831. https://doi.org/10.3390/md9050819
Mo, E., Moghadasi, Z., Rabbani, M., Ma, E., Samadi, S. and Mossaffa, N. (2012) Anticancer Effect of Dunaliella salina under Stress and Normal Conditions against Skin Carcinoma Cell Line A431 in Vitro. Iranian Journal of Fisheries Sciences, 11, 283-293.
Khayyal, M.T., El-Baz, F.K., Meselhy, M.R., Ali, G.H. and El-Hazek, R.M. (2019) Intestinal Injury Can Be Effectively Prevented by Dunaliella salina in Gamma Irradiated Rats. Heliyon, 5, e01814. https://doi.org/10.1016/j.heliyon.2019.e01814
Raja, R., Hemaiswarya, S., Balasubramanyam, D. and Rengasamy, R. (2007) Protective Effect of Dunaliella salina (Volvocales, Chlorophyta) against Experimentally Induced Fibrosarcoma on Wistar Rats. Microbiological Research, 162, 177-184. https://doi.org/10.1016/j.micres.2006.03.009
Oren, A. (1999) Benjamin Elazari Volcani (1915-1999): Sixty-Three Years of Studies of the Microbiology of the Dead Sea. International Microbiology, 2, 195-198. https://doi.org/10.1007/s007920050113
Oren, A. and Ventosa, A. (1999) In Memoriam-Benjamin Elazari Volcani. International Journal of Salt Lake Research, 8, 3-6. https://doi.org/10.1007/BF02442132
Rayappan, F. and Nair, A. (2013) Halophilic Bacteria-A Potent Source of Carotenoids with Antioxidant and Anticancer Potentials. Journal of Pure and Applied Microbiology, 7, 2825-2830.