Impact of MGMT Promoter Methylation as a Prognostic Marker in Patients with High Grade Glioma: A Single-Center Observational Study
- 1 Department of Medical Oncology, Health Care Global Enterprises Ltd., Bangalore, India
- 2 Department of Medical Oncology, Health Care Global Enterprises Ltd., Bangalore, India
- 3 Department of Medical Oncology, Health Care Global Enterprises Ltd., Bangalore, India
- 4 Department of Medical Oncology, Health Care Global Enterprises Ltd., Bangalore, India
- 5 Department of Medical Oncology, Health Care Global Enterprises Ltd., Bangalore, India
- 6 Department of Medical Oncology, Health Care Global Enterprises Ltd., Bangalore, India
- 7 Department of Medical Oncology, Health Care Global Enterprises Ltd., Bangalore, India
- 8 Department of Medical Oncology, Health Care Global Enterprises Ltd., Bangalore, India
Abstract
Objectives: 1) To correlate the methylation status of the O - 6-methylguanine- DNA-methyltransferase (MGMT promoter gene) and response to alkylating agent - based treatment in high - grade gliomas. Background: The MGMT gene is epigenetically silenced by promoter hypermethylation in gliomas and this modification has emerged as a relevant predictor of therapeutic response. Methods: 20 cases of high - grade glioma were analyzed for MGMT promoter methylation by methylation-specific PCR. Response to treatment and overall survival data w ere recorded and data analysed . Results: MGMT promoter methylation was found in 60% of gliomas by methylation-specific PCR. The mean survival time of glioblastoma patients submitted to adjuvant therapy was significantly higher among patients with MGMT promoter methylation (P = 0.035) and methylation status was an independent predictive factor that was associated with improved prognosis. Discussion and Conclusion: MGMT promoter methylation status was a more reliable predictor of response to adjuvant therapy and prognosis of high - grade gliomas. A subset of patients received irinotecan and bevacizumab in the second line setting and patients with unmethylated MGMT seemed to do better than the MGMT promoter methylated group.
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