The Activity of Serum 8-Iso-Prostaglandin F2<i>α</i> as Oxidative Stress Marker in Patients with Diabetes Mellitus Type 2 and Associated Dyslipidemic Hyperglycemia — Oak Academic Publishing
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The Activity of Serum 8-Iso-Prostaglandin F2<i>α</i> as Oxidative Stress Marker in Patients with Diabetes Mellitus Type 2 and Associated Dyslipidemic Hyperglycemia
Biochemistry Department, Faculty of Medicine, Umm Al-Qura University, Makkah, Saudi Arabia
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Gastroenterology Center, Faculty of Medicine, Mansoura University, Mansoura, Egypt
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Biochemistry Department, Faculty of Medicine, Umm Al-Qura University, Makkah, Saudi Arabia
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Biochemistry Department, Faculty of Medicine, Umm Al-Qura University, Makkah, Saudi Arabia
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Molecular Biology Department, Genetic Engineering and Biotechnology Research Institute, Sadat City University, Sadat City, Cairo, Egypt
1 Biochemistry Department, Faculty of Medicine, Umm Al-Qura University, Makkah, Saudi Arabia
2 Gastroenterology Center, Faculty of Medicine, Mansoura University, Mansoura, Egypt
3 Biochemistry Department, Faculty of Medicine, Umm Al-Qura University, Makkah, Saudi Arabia
4 Biochemistry Department, Faculty of Medicine, Umm Al-Qura University, Makkah, Saudi Arabia
5 Molecular Biology Department, Genetic Engineering and Biotechnology Research Institute, Sadat City University, Sadat City, Cairo, Egypt
Background: Oxidative stress has been closely linked to the incidence of diabetic complications. Therefore, the aim of this research article was to study hyperglycemia and abnormal lipid profile in diabetic patient type 2 and its correlation with oxidative stress development as measured by 8-iso-PGF2 α and 8-OHdG. Methods: Fifty (50) patients confirmed type 2 diabetes mellitus and eighty (80) non-diabetic control individuals were included in this study. All individuals were tested for blood glucose, lipid profile, 8-iso-PGF2 α and 8-OHdG HdG. Results: The age of diabetic patients was observed to be ≥40 yrs in 96% and diabetes was frequently detected in female than in male patients (76% vs . 24%, p < 0.0001). Mean serum lipids w ere elevated in diabetic patients compared with control individuals (p < 0.0001) except in HDL-C, a significant decrease was recorded (p = 0.04). Serum 8-iso-PGF2 α and 8-OHdG w ere elevated significantly in diabetic patients compared with non-diabetic control and a significant correlation was recorded between them (r = 0.6, p < 0.0001). 8-iso-PGF2 α was associated with Age (r = 0.394, p < 0.0001), FBG (0.553, p < 0.0001), LDL-C (r = 0.2, p = 0.023), TG (r = 0.176, p = 0.045) and TC (r = 0.2, p = 0.02). Also, 8-OHdG was associated with age (r = 0.558, p < 0.0001), FBG (r = 0.67, p < 0.0001), LDL-C (r = 0.28, p = 0.001), TG (r = 0.358, p < 0.0001) and TC (r = 0.33, p < 0.0001). Age, FBG, HbA1c, LDL-C, TG and TC showed a significant linear regression with 8-iso-PGF2 α and 8-OHdG recording its role as significant predictors for the elevation of 8-iso-PGF2 α and 8-OHdG. Therefore, hyperglycemia with oxidative stress development may play a role for dyslipidemia and diabetic complications. Conclusion: Diabetic patient’s type 2 has a higher rate of abnormal serum lipids and correlat es significantly with lipid peroxidation and oxidized DNA bases as measured by 8-iso-PGF2 α and 8-OHdG. Therefore, 8-iso-PGF2 α and 8-OHdG could be used as oxidative biomarkers for evaluating diabetic patients with early prediction of its complications and cancer development.
KeywordsDiabetes Mellitus Type 28-iso-PGF2<i>α</i>8-OHdGELISA
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