Diabetes mellitus is a group of metabolic disorders of carbohydrate metabolism in which glucose is underutilized, producing hyperglycaemia. The management requires proper maintenance of glucose and electrolytes within its optimum concentrations. The study was designed to evaluate the possibility of using saliva as an alternative non-invasive sample for the determination of electrolytes. A total of 100 samples were utilized consisting of equal number of control (non-diabetics) and diabetic groups. Fasting blood and saliva were collected employing standard methods. The biochemical parameters were analysed using WHO approved methods and procedures. Independent samples t-test and Pearson correlation were the statistical tools used for the data analysis obtained from SPSS package (version 20). The study revealed a significant increase (p < 0.05) in concentrations of blood and salivary glucose, potassium and calcium when controls were compared to diabetics. Moreover, there was a high level of semblances and patterns between plasma and salivary electrolytes, except for potassium. Therefore, electrolytes and glucose results gotten from the use of saliva could be used to equate to that of blood. Hence, instances of non-accessibility of blood, saliva could be of help.
Ashwood, E.R., Bruns, D.E. and Burtis, A.C. (2012) Carbohydrates. In: Tietz Fundamentals of Clinical Chemistry, 7th Edition, Elsevier, New Delhi, 373-395.
Agoro, E.S., Wankasi, M.M. and Illeimokum, O. (2015) Relationship between Serum Anion Gap and Diabetes Mellitus. Journal of Diabetes Mellitus, 5, 199-205. https://doi.org/10.4236/jdm.2015.54024
Baker, F.J., Silverton, R.E. and Pallister, C.J. (2001) Gastric and Pancreatic Function Tests. In: Introduction to Medical Laboratory Technology, 7th Edition, Edward Arnold, London, 103-129.
Knip, M., Virtanen, S.M. and Akerblom, H.K. (2010) Guidelines on Diabetes, Prediabetes, and Cardiovascular Diseases Developed in Collaboration. European Heart Journal, 91, 1506-1513.
Mitsumari, M., Yamaguchi, M. and Kano, Y.A. (1998) A New Approach to Non-Invasive Measurement of Blood Glucose Using Saliva Analyzing System. England Medicine and Biological Society, 4, 1767-1770.
Chávez, E.M., Borell, L.N., Taylor, G.W. and Ship, J.A. (2001) A Longitudinal Analysis of Salivary Flow in Control Subjects and Old Adults with Type 2 Diabetes. Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics, 91, 166-173. https://doi.org/10.1067/moe.2001.112054
Pal, P., Desai, N.T., Kannan, N., Masur, V.N., Daniel, M.J. and Bhatt, N. (2003) Estimation of Salivary Glucose, Salivary Amylase, Salivary Total Protein and Periodontal Microflora in Diabetes Mellitus. Journal of the Indian Dental Association, 74, 143-149.
Lopez, M.E., Colloca, M.E., Paez, R.G., Schallmach, J.N., Koss, M.A. and Chervonagura, A. (2003) Salivary Characteristics of Diabetic Children. Brazilian Dental Journal, 14, 26-31. https://doi.org/10.1590/S0103-64402003000100005
Amer, S., Yousuf, M., Siddiqiui, P.Q. and Alam, J. (2001) Salivary Concentrations in Patients with Diabetes Mellitus—A Minimally Invasive Technique for Monitoring Blood Glucose Levels. Pakistan Journal of Pharmaceutical Science, 14, 33-37.
Agoro, E.S., Nelson-Ebimie, E., Soroh, A.E. and Odegbemi, J.O. (2017) The Suitability of Non-Invasive Sample in the Assay of Glucose in Diabetes Mellitus Diagnosis and Sex Difference. Journal of Applied Microbiology and Biochemistry, 2, 6.
Agoro, E.S., Ebiere, N.E., Eseimokumo, S.A. and Odegbemi, J.O. (2017) Is Saliva an Alternative Non-Invasive Sample for the Estimation of Protein Profile amongst Diabetics and Gender-Based Diagnostics? Anatomy and Physiology: Current Research, 7, 255.
Nielsen, M. (1995) Ions: The Body’s Electrical Energy Source. Mineral Resources International, 9, 178-183.
Horne, M.M. and Swearingen, P.L. (1989) Fluids, Electrolytes, and Acid-Base Balance. In: Year Book, 2nd Edition, Mosby, Saint Louis, 64-69.
De Almeida, P.V., Gregio, A.M., Machado, M.A., De Lima, A.A. and Azevedo, L.R. (2008) Saliva Composition and Functions: A Comprehensive Review. Journal Contemporary Dental Practice, 9, 72-80.
Ahmadi, M.F., Davoodi, P. and Dalband, M. (2010) Saliva as a Mirror of the Body Health. DJH, 1, 1-15.
Sreedevi, S.M.C. and Shambulingappa, P. (2008) Comparison of Serum Glucose and Salivary Glucose in Diabetic Patients. Journal of Indian Academy of Oral Medicine and Radiology, 20, 9-13. https://doi.org/10.4103/0972-1363.44354
John, O.O. and Arundhati, A.K. (2007) Carbohydrate Metabolism. In: Medical Laboratory Science Theory and Practice, 6th Edition, Tata McGraw-Hill, New Delhi, 99-110.
Carlos, L., Silke, T., John, P., Mohamed, T., Simon, N.T., Athanase, B. and Magnus, B. (2018) Differential Associations for Salivary Sodium, Potassium, Calcium, and Phosphate Levels with Carotid Intima Media Thickness, Heart Rate, and Arterial Stiffness. Disease Markers, 2018, Article ID: 3152146. https://doi.org/10.1155/2018/3152146
Shirzaiy, M., Heidari, F., Dalirsani, Z. and Dehghan, J. (2015) Estimation of Salivary Sodium, Potassium, Calcium, Phosphorus and Urea in Type II Diabetic Patients. Diabetes and Metabolic Syndrome: Clinical Research and Reviews, 9, 332-336. https://doi.org/10.1016/j.dsx.2013.02.025
Nerea, B.T., Ramón, E., Mònica, B., Rosa, C., Andrés, D.L., Josep, B., Montserrat, F., Lluis, S.M. and Jordi, S.S. (2014) Increased Serum Calcium Levels and Risk of Type 2 Diabetes in Individuals at High Cardiovascular Risk. Diabetes Care, 37, 3084-3091. https://doi.org/10.2337/dc14-0898
Sharon, A., Hannah, B.A., Biran, I., Yoram, K., Szargel, R., Gutman, D., et al. (1985) Salivary Composition in Diabetic Patients. Journal of Oral Medicine, 40, 23-26.
Antonios, H.T. and Pratap, S.A. (1987) Serum Potassium Concentration in Hyperglycemia of Diabetes Mellitus with Long-Term Dialysis. Western Journal of Medicine, 146, 571-575.
Ben-Aryeh, H., Serouya, R., Kanter, Y., Szargel, R. and Laufer, D. (1993) Oral Health and Salivary Composition in Diabetic Patients. Journal of Diabetes and Its Complications, 7, 57-62. https://doi.org/10.1016/1056-8727(93)90025-T
Yavuzyilmaz, E., Yumak, O., Akdoganli, T., Yamalik, N., Ozer, N., Ersov, F, et al. (1996) The Alteration of Whole Saliva Constituents in Patients with Diabetes Mellitus. Australian Dental Journal, 41, 193-197. https://doi.org/10.1111/j.1834-7819.1996.tb04855.x
Marder, M.Z., Abelson, D.C. and Mandel, I.D. (1975) Salivary Alterations in Diabetes Mellitus. Journal of Periodontology, 46, 567-569. https://doi.org/10.1902/jop.1975.46.9.567
Streckfus, C.F., Marcus, S., Welsh, S., Brown, R.S., Cherry-Peppers, G. and Brown, R.H. (1994) Parotidfunction and Composition of Parotid Saliva among Elderly Edentulous African-American Diabetics. Journal of Oral Pathology & Medicine, 23, 277-279. https://doi.org/10.1111/j.1600-0714.1994.tb00059.x
Moore, P.A., Guggenheimer, J., Etzel, K.R., Weyant, R.J. and Orchard, T. (2001) Type 1 Diabetes Mellitus, Xerostomia and Salivay Flow Rates. Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontology, 92, 281-291. https://doi.org/10.1067/moe.2001.117815
Prakash, C.K., Seraj, A.K. and Rajendra, K.C. (2014) Salivary Glucose Levels in Diabetic Patients: A Comparative Cross Sectional Study. International Journal of Therapeutic Applications, 18, 12-17.