<i>In Vitro</i> Studies of NIPAAM-MAA-VP Copolymer-Coated Magnetic Nanoparticles for Controlled Anticancer Drug Release*

Abstract

Thermosensetive poly(N-isopropylacrylamide)-based magnetic nanoparticles were synthesized by free radical polym erization of N-isopropylacrylamide (NIPPAMs), methacrylic acid (MAA), and vinyl pyrrolidone (VP) in the presence of methylene-bis-acrylamide as cross linking agent. The Fe 3 O 4 magnetic nanoparticls were prepared by chemical pre cipitation of Fe salts in the ratio of 1:2 under alkaline and inert condition. Thermosensitive crosslinked P (NI - PAAM-MAA-VP) copolymer s were characterized by FT-IR and H-NMR . The pH and thermosensitive copolymer was used for preparation of drug loaded magnetic nanoparticles , and doxorubicin (DOX) was used as a typical anticancer drug. The amount of the loaded drug and drug release amount were determined by UV measurements. Scanning elec tron microscopy (SEM) and lower critical solution temperature (LCST) were used to determine the particle surface morphology and the phase transition temperature of the nanoparticles respectively. The release behavior of DOX at pH = 7.4 and 37°C was studied. The result indicated that this thermosensetive magnetic nanoparticle has a high drug load ing capacity and favorable linear release property for DOX without initial burst release. Thus this system is promising for the application in targeted smart anticancer drug delivery.