Genotoxicity of Water Extracts from Sewage Effluents in the Kanagawa Prefecture, Japan Using the Novel <i>umu</i> Tester Strain — Oak Academic Publishing
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Genotoxicity of Water Extracts from Sewage Effluents in the Kanagawa Prefecture, Japan Using the Novel <i>umu</i> Tester Strain
Department of Applied Chemistry, Kanagawa Institute of Technology, Atsugi, Japan
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Department of Applied Chemistry, Kanagawa Institute of Technology, Atsugi, Japan
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Institute of Life and Environmental Sciences, Osaka Shin-Ai College, Osaka, Japan
1 Department of Applied Chemistry, Kanagawa Institute of Technology, Atsugi, Japan
2 Department of Applied Chemistry, Kanagawa Institute of Technology, Atsugi, Japan
3 Institute of Life and Environmental Sciences, Osaka Shin-Ai College, Osaka, Japan
The genotoxic activities of effluents from drainage water treatment plants were examined by using the novel umu tester strain NM8001, which lacks MutMst genes. To enhance the sensitivity of the LacZ assay, a BugBuster mix protein extraction reagent and TokyoGreen- β Gal for a fluorescence-galactosidase substrate were applied. Of the 24 sampling locations present in Kanagawa prefecture, Japan, water extracts from nine sampling points showed apparent genotoxic activities without metabolic activation. In contrast, water extracts from the upper sites of these water treatment plants did not show any significant genotoxic activities. The selected samples with genotoxic activity did not show significant mutagenicity toward Ames strains TA98 and TA100. Genotoxicity was also well correlated with the activity of a classical umu strain of TA1535/pSK1002; these findings indicate that the genotoxicity induced by oxidative damage was not a significant component of the genotoxicity.
KeywordsGenotoxic CompoundsMutagenic CompoundsSurface Water Contamination<i>umu</i>Tester Strain
Murphy, E.A., Post, G.B., Buckley, B.T., Lippincott, R.L. and Robson, M.G. (2012) Future Challenges to Protecting Public Health from Drinking-Water Contaminants. Annual Review of Public Health, 33, 209-224. https://doi.org/10.1146/annurev-publhealth-031811-124506
Sun, Z., Sokolova, E., Brittain, J.E., Saltveit, S.J., Rauch, S. and Meland, S. (2019) Impact of Environmental Factors on Aquatic Biodiversity in Roadside Stormwater Ponds. Scientific Reports, 9, Article No. 5994. https://doi.org/10.1038/s41598-019-42497-z
Wee, S.Y. and Aris, A.Z. (2019) Occurrence and Public-Perceived Risk of Endocrine Disrupting Compounds in Drinking Water. NPJ Clean Water, 2, 4. https://doi.org/10.1038/s41545-018-0029-3
Canada, C.C., Blaise, C. and Canada, E. (2004) Effects of Pharmaceuticals on Aquatic Biota—A Review. Current Topics in Toxicology, 1, 73-86.
Koelmans, A.A., Mohamed Nor, N.H., Hermsen, E., Kooi, M., Mintenig, S.M. and De France, J. (2019) Microplastics in Freshwaters and Drinking Water: Critical Review and Assessment of Data Quality. Water Research, 155, 410-422. https://linkinghub.elsevier.com/retrieve/pii/S0043135419301794 https://doi.org/10.1016/j.watres.2019.02.054
Makowska, M. and Spychala, M. (2014) Organic Compounds Fractionation for Domestic Wastewater Treatment Modeling. Polish Journal of Environmental Studies, 23, 131-137.
Schaider, L.A., Rodgers, K.M. and Rudel, R.A. (2017) Review of Organic Wastewater Compound Concentrations and Removal in Onsite Wastewater Treatment Systems. Environmental Science and Technology, 51, 7304-7317. https://doi.org/10.1021/acs.est.6b04778
Altenburger, R., Brack, W., Burgess, R.M., Busch, W., Escher, B.I., Focks, A., et al. (2019) Future Water Quality Monitoring: Improving the Balance between Exposure and Toxicity Assessments of Real-World Pollutant Mixtures. Environmental Sciences Europe, 31, 1-17. https://doi.org/10.1186/s12302-019-0193-1
Ohe, T., Watanabe, T. and Wakabayashi, K. (2004) Mutagens in Surface Waters: A Review. Mutation Research, 567, 109-149. http://www.ncbi.nlm.nih.gov/pubmed/15572284 https://doi.org/10.1016/j.mrrev.2004.08.003
Sakamoto, H. and Hayatsu, H. (1990) A Simple Method for Monitoring Mutagenicity of River Water. Mutagens in Yodo River System, Kyoto-Osaka. Bulletin of Environmental Contamination and Toxicology, 44, 521-528. http://www.ncbi.nlm.nih.gov/pubmed/2182143 https://doi.org/10.1007/BF01700870
Takamura-Enya, T., Ishii, R. and Oda, Y. (2011) Evaluation of Photo-Genotoxicity Using the umu Test in Strains with a High Sensitivity to Oxidative DNA Damage. Mutagenesis, 26, 499-505. https://doi.org/10.1093/mutage/ger008 http://www.ncbi.nlm.nih.gov/pubmed/21478297
Ohe, T., Mizuno, T., Morisawa, T., Kiritani, S., Suzuki, S., Takehana, H., et al. (2006) Mutagenicity and Levels of 2-Phenylbenzotriazole (PBTA)-Type Mutagens in Sewage Effluent, River Water, Sediment and Drinking Water Collected from the Yodo River System, Japan. Genes and Environment, 28, 108-119. https://doi.org/10.3123/jemsge.28.108
Takamura-Enya, T., Watanabe, T., Tada, A., Hirayama, T., Nukaya, H., Sugimura, T., et al. (2002) Identification of a New Mutagenic Polychlorinated Biphenyl Derivative in the Waka River, Wakayama, Japan, Showing Activation of an Aryl Hydrocarbon Receptor-Dependent Transcription. Chemical Research in Toxicology, 15, 419-425. http://www.ncbi.nlm.nih.gov/pubmed/11896690 https://doi.org/10.1021/tx010163g
Hartmann, A., Alder, A.C., Koller, T. and Widmer, R.M. (1998) Identification of Fluoroquinolone Antibiotics as the Main Source of umuC Genotoxicity in Native Hospital Wastewater. Environmental Toxicology and Chemistry, 17, 377-382. https://doi.org/10.1002/etc.5620170305
Tsukatani, H., Tanaka, Y., Sera, N., Shimizu, N., Kitamori, S. and Inoue, N. (2003) Validity of Mutagenic Activity as an Indicator of River Water Pollution. Environmental Health and Preventive Medicine, 8, 133-138. https://doi.org/10.1007/BF02897917
Tian, Z., Oda, Y., Zhang, Y., Yang, M. and Li, H. (2015) Use of a New Enzyme Extraction System to Improve the Sensitivity of SOS/umu Test and Application to Environmental Samples. Bulletin of Environmental Contamination and Toxicology, 94, 370-375. https://doi.org/10.1007/s00128-014-1445-9
Reifferscheid, G., Heil, J., Oda, Y. and Zahn, R.K. (1991) A Microplate Version of the SOS/umu-Test for Rapid Detection of Genotoxins and Genotoxic Potentials of Environmental Samples. Mutation Research/Environmental Mutagenesis and Related Subjects, 253, 215-222. https://doi.org/10.1016/0165-1161(91)90134-T
Oda, Y., Yamazaki, H., Thier, R., Ketterer, B., Guengerich, F.P. and Shimada, T. (1996) A New Salmonella typhimurium NM5004 Strain Expressing Rat Glutathione S-Transferase 5-5: Use in Detection of Genotoxicity of Dihaloalkanes Using an SOS/umu Test System. Carcinogenesis, 17, 297-302. http://www.ncbi.nlm.nih.gov/pubmed/8625454 https://doi.org/10.1093/carcin/17.2.297
Schindler Wildhaber, Y., Mestankova, H., Scharer, M., Schirmer, K., Salhi, E. and von Gunten, U. (2015) Novel Test Procedure to Evaluate the Treatability of Wastewater with Ozone. Water Research, 75, 324-335. https://doi.org/10.1016/j.watres.2015.02.030
Kameya, T., Nagato, T., Nakagawa, K., Yamashita, D., Kobayashi, T. and Fujie, K. (2011) Quantification of umu Genotoxicity Level of Urban River Water. Water Science and Technology, 63, 410-415. https://iwaponline.com/wst/article/63/3/410/13961/Quantification-of-umu-genotoxicity-level-of-urban https://doi.org/10.2166/wst.2011.235
Rincón-Bedoya, E., Velásquez, N., Quijano, J. and Bravo-Linares, C. (2013) Mutagenicity and Genotoxicity of Water Treated for Human Consumption Induced by Chlorination By-Products. Journal of Environmental Health, 75, 28-36.
Ono, Y., Somiya, I. and Kawamura, M. (1991) The Evaluation of Genotoxicity Using DNA Repairing Test for Chemicals Produced in Chlorination and Ozonation Processes. Water Science and Technology, 23, 329-338. https://iwaponline.com/wst/article/23/1-3/329/26650/The-Evaluation-of-Genotoxicity-Using- DNA-Repairing https://doi.org/10.2166/wst.1991.0431