This study assessed the levels of oxidative stress biomarkers in gills and liver, as well as the activities of transaminases in the liver of Nile tilapia ( Oreochromis niloticus ), exposed to pharmaceutical effluents. The pharmaceutical effluents were collected from two pharmaceutical industries in Lagos, Nigeria. The assessment of physicochemical characteristics of the effluents indicated that some parameters were not in accordance with NESREA limits. The acute toxicity studies showed that 96hrLC 50 values of “effluent A” and “effluent B” were 27.0 ml/L and 18.0 ml/L respectively. The juveniles of O. niloticus were exposed to 1/100 th and 1/10 th LC 50 s of the two effluents for a period of 14 and 28 days. These concentrations significantly increased the level of the lipid peroxidation product, malondialdehyde. There was also inhibition of the activities of superoxide dismutase and catalase as well as significantly lower levels of reduced glutathione after 28 days. The levels of the transaminases (aspartate aminotransferase and alanine transaminase) were elevated in the liver of the fish after the exposure to the effluents. The present findings showed that the wastewater caused oxidative stress and hepatocellular damage in the fish suggesting potential ecotoxicological risks of the wastewater to aquatic organisms.
Anyakora, C., Nwaeze, K., Awodele, O., Nwadike, C., Arbabi, M. and Coker, H. (2011) Concentrations of Heavy Metals in Some Pharmaceutical Effluents in Lagos, Nigeria. Journal of Environmental Chemistry and Ecotoxicology, 3, 25-31.
Obasi, A.I., Amaeze, N.H. and Osoko, D.D. (2014) Microbiological and Toxicological Assessment of Pharmaceutical Wastewater from the Lagos Megacity, Nigeria. Chinese Journal of Biology, 2014, Article ID: 638142. https://doi.org/10.1155/2014/638142
Idris, M.A., Kolo, B.G., Garba, S.T. and Ismail, M.A. (2013) Physicochemical Analysis of Pharmaceutical Effluent and Surface Water of River Gorax in Minna, Niger State, Nigeria. Bulletin of Environment, Pharmacology and Life Sciences, 2, 45-49.
Ibegbulam-Njoku, P.N., Chijioke-Osuji, C.C. and Imo, E.O. (2013) Physicochemical Characteristics and Biodegradation of Pharmaceutical Effluents. International journal of Scientific & Engineering Research, 4, 29.
Ohlenbusch, G., Kumke, M.U. and Frimmel, F.H. (2000) Sorption of Phenols to Dissolved Organic Matter Investigated by Solid Phase Microextraction. Science of the Total Environment, 253, 63-74. https://doi.org/10.1016/S0048-9697(00)00376-4
Cleuvers, M. (2003) Aquatic Ecotoxicity of Pharmaceuticals Including the Assessment of Combination Effects. Toxicology Letters, 142, 185-194. https://doi.org/10.1016/S0378-4274(03)00068-7
Lateef, A., Yekeen, T.A. and Ufuoma, P.E. (2007) Bacteriology and Genotoxicity of Some Pharmaceutical Wastewaters in Nigeria. International Journal of Environment and Health, 1, 551-562. https://doi.org/10.1504/IJENVH.2007.018572
Akintonwa, A., Awodele, O., Olofinnade, A.T., Anyakora, C., Afolayan, G.O. and Coker, H.A.B. (2009) Assessment of the Mutagenicity of Some Pharmaceutical Effluents. American Journal of Pharmacology and Toxicology, 4, 144-150. https://doi.org/10.3844/ajptsp.2009.144.150
Bakare, A.A., Okunola, A.A., Adetunji, O.A. and Jenmi, H.B. (2009) Genotoxicity Assessment of a Pharmaceutical Effluent Using Four Bioassays. Genetics and Molecular Biology, 32, 373-381. https://doi.org/10.1590/S1415-47572009000200026
Chang, Y.C., Chen, C.H. and Lin, M.C. (2012) The Macronutrients in Human Milk Change after Storage in Various Containers. Pediatrics & Neonatology, 53, 205-209. https://doi.org/10.1016/j.pedneo.2012.04.009
Timbrell, J. (2000) Principles of Biochemical Toxicology. 3rd Edition, Taylor & Francis, Abingdon-on-Thames.
Obanya, H.E., Omoarukhe, A., Amaeze, N.H. and Okoroafor, C.U. (2019) Polycyclic Aromatic Hydrocarbons in Ologe Lagoon and Effects of Benzo[b]fluoranthene in African Catfish. Journal of Health and Pollution, 9, Article ID: 190605. https://doi.org/10.5696/2156-9614-9.22.190605
Larsson, D.J., de Pedro, C. and Paxeus, N. (2007) Effluent from Drug Manufactures Contains Extremely High Levels of Pharmaceuticals. Journal of Hazardous Materials, 148, 751-755. https://doi.org/10.1016/j.jhazmat.2007.07.008
Jones, O.A.H., Voulvoulis, N. and Lester, J.N. (2001) Human Pharmaceuticals in the Aquatic Environment a Review. Environmental Technology, 22, 1383-1394. https://doi.org/10.1080/09593330.2001.11090873
Adeoye, G.O., Alimba, C.G. and Oyeleke, O.B. (2015) The Genotoxicity and Systemic Toxicity of a Pharmaceutical Effluent in Wistar Rats May Involve Oxidative Stress Induction. Toxicology Reports, 2, 1265-1272. https://doi.org/10.1016/j.toxrep.2015.09.004
Babatunde, A., Bamgbola, E.P. and Oyelola, O. (2014) The Effect of Pharmaceutical Effluents on the Quality of Groundwater: A Case Study of Ikeja Industrial Area of Lagos, Nigeria. International Journal of Research in Medical and Health Sciences, 4, 1.
Kumari, V. and Tripathi, A.K. (2019) Characterization of Pharmaceuticals Industrial Effluent Using GC-MS and FT-IR Analyses and Defining Its Toxicity. Applied Water Science, 9, 185. https://doi.org/10.1007/s13201-019-1064-z
Obayiuwana, A., Ogunjobi, A., Yang, M. and Ibekwe, M. (2018) Characterization of Bacterial Communities and Their Antibiotic Resistance Profiles in Wastewaters Obtained from Pharmaceutical Facilities in Lagos and Ogun States, Nigeria. International Journal of Environmental Research and Public Health, 15, Article No. 1365. https://doi.org/10.3390/ijerph15071365
APHA (American Public Health Association) (2005) Standard Methods for the Examination of Water and Wastewater. 21st Edition, American Public Health Association, Washington DC.
Amaeze, N.H., Adeyemi, R.O. and Adebesin, A.O. (2015) Oxidative Stress, Heats Shock Protein and Histopathological Effects in the Gills of African Catfish, Clarias gariepinus Induced by Bridge Runoffs. Environmental Monitoring and Assessment, 187, 172. https://doi.org/10.1007/s10661-015-4390-0
Sedlak, J. and Lindsay, R.H. (1968) Estimation of Total, Protein-Bound, and Non-Protein Sulfhydryl Groups in Tissue with Ellman’s Reagent. Analytical Biochemistry, 25, 192-205. https://doi.org/10.1016/0003-2697(68)90092-4
Sun, M. and Zigma, S. (1978) An Improved Spectrophotometer Assay of Superoxide Dismutase Based on Epinephrine Antioxidation. Analytical Biochemistry, 90, 81-89. https://doi.org/10.1016/0003-2697(78)90010-6
Sinha, A.K. (1957) Colorimetric Assay of Catalase. Analytical Biochemistry, 47, 389-394. https://doi.org/10.1016/0003-2697(72)90132-7
Buege, J.A. and Aust, S.D. (1978) Microsomal Lipid Peroxidation. Methods in Enzymology, 52, 302-310. https://doi.org/10.1016/S0076-6879(78)52032-6
Reitman, S. and Frankel, S. (1957) A Colorimetric Method for the Determination of Serum Glutamic Oxalacetic and Glutamic Pyruvic Transaminase. American Journal of Clinical Pathology, 28, 56-63. https://doi.org/10.1093/ajcp/28.1.56
NESREA (National Environmental Standards and Regulations Enforcement Agency) (2009) Maximum Permissible Limits for Wastewater Discharge. The Federal Government Press, Abuja.
Olaitan, J.O., Oluwaleye, S.E., Saka, K.L. and Olabanji, D.J. (2014) Physico-Chemical Characteristics of Pharmaceutical Effluents from Sango Industrial Area, Nigeria. Bulletin of Environment, Pharmacology and Life Sciences, 3, 78-81.
De Schamphelaere, K.A., Koene, J.M., Heijerick, D.G. and Janssen, C.R. (2008) Reduction of Growth and Haemolymph Ca Levels in the Freshwater Snail Lymnaea stagnalis Chronically Exposed to Cobalt. Ecotoxicology and Environmental Safety, 71, 65-70. https://doi.org/10.1016/j.ecoenv.2007.07.004
Dringen, R. (2000) Metabolism and Functions of Glutathione in Brain. Progress in Neurobiology, 62, 649-671. https://doi.org/10.1016/S0301-0082(99)00060-X
Jana, S.K., Dutta, M., Joshi, M., Srivastava, S., Chakravarty, B. and Chaudhury, K. (2013) 1H NMR Based Targeted Metabolite Profiling for Understanding the Complex Relationship Connecting Oxidative Stress with Endometriosis. BioMed Research International, 2013, Article ID: 329058. https://doi.org/10.1155/2013/329058
Ryu, B., Himaya, S.W.A., Qian, Z.J., Lee, S.H. and Kim, S.K. (2011) Prevention of Hydrogen Peroxide-Induced Oxidative Stress in HDF Cells by Peptides Derived from Seaweed Pipefish, Syngnathus schlegeli. Peptides, 32, 639-647. https://doi.org/10.1016/j.peptides.2011.01.009
Gallagher, E.P., Hasspieler, B.M. and Di Glulio, R.T. (1992) Effects of Buthionine Sulfoximine and Diethyl Maleate on Glutathione Turnover in the Channel Catfish. Biochemical Pharmacology, 43, 2209-2215. https://doi.org/10.1016/0006-2952(92)90180-Q
Zhang, J., Shen, H., Wang, X., Wu, J. and Xue, Y. (2004) Effects of Chronic Exposure of 2,4-Dichlorophenol on the Antioxidant System in Liver of Freshwater Fish Carassius auratus. Chemosphere, 55, 167-174. https://doi.org/10.1016/j.chemosphere.2003.10.048
Kovarova, J., Celechovska, O., Kizek, R., Adam, V., Harustiakova, D. and Svobodova, Z. (2009) Effect of Metals, with Special Attention of Cd, Content of the Svitava and Svratka Rivers on Levels of Thiol Compounds in Fish Liver and Their Use as Biochemical Markers. Neuroendocrinology Letters, 30, 169-176.
Cao, L., Huang, W., Liu, J., Yin, X. and Dou, S. (2010) Accumulation and Oxidative Stress Biomarkers in Japanese Flounder Larvae and Juveniles under Chronic Cadmium Exposure. Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 151, 386-392. https://doi.org/10.1016/j.cbpc.2010.01.004
Jia, X., Zhang, H. and Liu, X. (2011) Low Levels of Cadmium Exposure Induce DNA Damage and Oxidative Stress in the Liver of Oujiang Colored Common Carp Cyprinus carpio var. Color. Fish Physiology and Biochemistry, 37, 97-103. https://doi.org/10.1007/s10695-010-9416-5
Arun, S., Thirumurugan, R., Visakan, R., Balamurugan, S., Arunachalam, V. and Subramanian, P. (2003) Optimal Analytical Conditions for Catalase in Fresh Water Prawn, Macrobrachium malcolmsonii. Biotechnic & Histochemistry, 78, 1-4. https://doi.org/10.1080/10520290312120001
Zhou, J.Y. and Prognon, P. (2006) Raw Material Enzymatic Activity Determination: A Specific Case for Validation and Comparison of Analytical Methods—The Example of Superoxide Dismutase (SOD). Journal of Pharmaceutical and Biomedical Analysis, 40, 1143-1148. https://doi.org/10.1016/j.jpba.2005.09.022
Timbrel, J.A. (2009) Principles of Biochemical Toxicology, 4th Edition, Informa Healthcare, New York.
Yancheva, V.S., Georgieva, E.S., Velcheva, I.G., Iliev, I.N., Vasileva, T.A., Petrova, S.T. and Stoyanova, S.G. (2014) Biomarkers in European Perch (Perca fluviatilis) Liver from a Metal-Contaminated Dam Lake. Biologia, 69, 1615-1624. https://doi.org/10.2478/s11756-014-0460-y
Baghshani, H. and Shahsavani, D. (2013) Effects of Lead Acetate Exposure on Metabolic Enzyme Activities in Selected Tissues of Common Carp (Cyprinus carpio). Comparative Clinical Pathology, 22, 903-907. https://doi.org/10.1007/s00580-012-1497-3
Nwani, C.D., Nnaji, M.C., Oluah, S.N., Echi, P.C., Nwamba, H.O., Ikwuagwu, O.E. and Ajima, M.N.O. (2014) Mutagenic and Physiological Responses in the Juveniles of African Catfish, Clarias gariepinus (Burchell 1822) Following Short Term Exposure to Praziquantel. Tissue and Cell, 46, 264-273. https://doi.org/10.1016/j.tice.2014.05.011