Bacterial Influence on Textile Wastewater Decolorization
- 1 Department of Hydrology and water resources, Hohai University,China, Nanjing
- 2 State Key Laboratory of Hydrology-Water Resources and Hydraulic Engineering, Hohai University,China, Nanjing
Abstract
The study aims to isolate and optimize bacterial strains having the ability to degrade and decolorize azo dyes produced in the final effluent of textile dying industries. In this regard, ten bacterial strains were isolated from wastewater treatment plants, and most of them were subjected to the colored effluents resulting from dilapidated houses. The ability of these bacterial isolations to use a wide range of azo dyes to determine the sole carbon source was determined. According to these screening testes, two bacterial isolations were selected as the most potent decolorizer for azo dyes, and they were identified as Comamanas acidovorns-TN1 and Burkholdera cepace-TN5. The optimization process started with the addition of 1 g/l yeast extract, where the decolorization ability of the two strains increased sharply and according to this experiment, the two azo dyes, Acid orange 7 and Direct blue 75, were selected to complete the study. The effect of different conditional and chemical factors on the decolorization process of Acid orange 7 and Direct blue 75 by Comamanas acidovorns-TN1 and Burkholdera cepace-TN5 was studied. Factors that contributed to the difference were different pH, temperature, incubation period, inoculum size, carbon source, nitrogen source and the respective concentrations of yeast extract. This study recommends the application of the two most potent bacterial strains in the decolorization of the azo dyes, along with acid orange 7 and direct blue 75, specifically in the industrial effluents under all nutritional and environmental conditions.
- C. M. Carliell, S. J. Barclay, N. Naidoo, C. A. Buckley, D. A. Mulholland and E. Senior, “Microbial Decolorization of a Reactive Azodye under Anaerobic Conditions,” Water SA, Vol. 21, No. 1, 1995, pp. 61-69.
- N. Dafale, S. Watea, S. Meshram and T. Nandya, “Kinetic Study Approach of Remazol Black-Buse for the Development of Two-Stage Anoxic-Oxic Reactor for Decolorization/Biodegradation of Azo Dyes by Activated Bacterial Consortium,” Journal of Hazardous Materials, Vol. 159, No. 2-3, 2008, pp. 319-328. doi:10.1016/j.jhazmat.2008.02.058
- A. M. Talarposhti, T. Donnelly and G. K. Anderson, “Colour Removal from a Simulated Dye Wastewater Using a Two Phase Anaerobic Packed Bed Reactor,” Water Research, Vol. 35, No. 2, 2001, pp. 425-432. doi:10.1016/S0043-1354(00)00280-3
- K. Wuhrmann, K. L. Mechsner and T. H. Kappeler, “Investigation on Rate Determining Factors in the Microbial Reduction of Azo Dyes,” Applied Microbiology and Biotechnology, Vol. 9, No. 4, 1980, pp. 325-338. doi:10.1007/BF00508109
- D. Deng, J. Guo, G. Zeng and G. Sun, “Decolorization of Anthraquinone, Triphenylmethane and Azo Dyes by a New Isolated Bacillus Cereus Strain DC11,” International Biodeterioration & Biodegradation, Vol. 62, No. 3, 2008, pp. 263-269.
- D. Brown and P. Laboureur, “The Degradation of Dyestuffs: Part I. Primary Biodegradation under Anaerobic Conditions,” Chemosphere, Vol. 12, No. 3, 1983, pp. 397-404. doi:10.1016/0045-6535(83)90114-5
- E. Razo-Flores, M. Luijten, B. Donlon, G. Lettinga and J. Field, “Complete Biodegradation of the Azo Dye Azosalicylate under Anaerobic Conditions,” Environmental Science and Technology, Vol. 31, No. 7, 1997, pp. 2098- 2103. doi:10.1021/es960933o
- S. Chinwetkitvanich, M. Tuntoolvest and T. Panswad, “Anaerobic Decolorization of Reactive Dyebath Effluents by a Two Stage UASB System with Tapioca as Co- Substrate,” Water Research, Vol. 34, No. 8, 2000, pp. 2223- 2232. doi:10.1016/S0043-1354(99)00403-0
- W. G. Levine, “Metabolism of Azo Dyes: Implication for Detoxification and Activation,” Drug Metabolism Review, Vol. 23, No. 3-4, 1991, pp. 253-309. doi:10.3109/03602539109029761
- F. He, W. R. Hu and Y. Z. Li, “Biodegradation Mechanisms and Kinetics of Azo Dye 4BS by a Microbial Consortium,” Chemosphere, Vol. 57, No. 4, 2004, pp. 293- 301. doi:10.1016/j.chemosphere.2004.06.036
- N. Hayase, K. Kouno and K. Ushio, “Isolation and Characterization of Aeromonas sp. B-5 Capable of Decolorizing Various Dyes,” Journal of Bioscience and Bioengineering, Vol. 90, No. 5, 2000, pp. 570-573.