Phthalate Metabolites in Amniotic Fluid and Maternal Urine Samples
- 1 INAIL Research, Monteporzio Catone, Italy
- 2 INAIL Research, Monteporzio Catone, Italy
- 3 INAIL Research, Monteporzio Catone, Italy
- 4 INAIL Research, Monteporzio Catone, Italy
- 5 INAIL Research, Monteporzio Catone, Italy
- 6 Gynaecology and Obstetrics II, Azienda Ospedaliera San Giovanni-Addolorata, Rome, Italy
- 7 INAIL Research, Monteporzio Catone, Italy
Abstract
The objective of this study was to determine the concentrations of the metabolites of four selected phthalates, widely used industrial chemicals which possess endocrine-disrupting properties, in samples of amniotic fluid and maternal urine collected in the same day, in order to verify if the latter can be considered a measure of the fetal exposure. The quantitative determination of the metabolites was carried out by HPLC-MS/MS with isotopic dilution from 70 pregnant volunteers. Detectable concentrations of phthalates metabolites were found in amniotic fluids. As phthalate monoesters are excreted in the urine conjugated with glucuronic acid, an enzymatic hydrolysis is carried out before analysis. Amniotic fluids were tested with and without hydrolysis and only the free phthalate metabolites, not conjugated with glucuronic acid, were found. The concentration of metabolites after enzymatic hydrolysis in maternal urine is not correlated to those of amniotic fluids, but the free form concentrations are. These results suggest that only the free forms can cross the placenta. A significant number of mothers showed urine phthalate monoesters concentrations higher than non-pregnant women.
- Angerer, J., Ewers, U. and Wilhelm, M. (2007) Human Biomonitoring: State of the Art. International Journal of Hygiene and Environmental Health, 210, 201-228. http://dx.doi.org/10.1016/j.ijheh.2007.01.024
- Barr, D.B., Bishop, A. and Needham, L.L. (2007) Concentrations of Xenobiotic Chemicals in the Maternal-Fetal Unit. Reproductive Toxicology, 23, 260-266. http://dx.doi.org/10.1016/j.reprotox.2007.03.003
- Foster, W.G., Hughes, C.L., Chan, S. and Platt, L. (2002) Human Developmental Exposure to Endocrine Active Compounds. Environmental Toxicology and Pharmacology, 12, 75-81. http://dx.doi.org/10.1016/S1382-6689(02)00025-X
- Foster, W.G., Chan, S., Platt, L. and Hughes, C.L. (2000) Detection of Endocrine Disrupting Chemicals in Samples of Second Trimester Human Amniotic Fluid. The Journal of Clinical Endocrinology and Metabolism, 85, 2954-2957. http://dx.doi.org/10.1210/jcem.85.8.6850
- Luzardo, O.P., Mahtani, V., Troyano, J.M., Alvarez de la Rosa, M. and Padilla-Perez, A.I. (2009) Determinants of Organochlorine Levels Detectable in the Amniotic Fluid of Women from Tenerife Island (Canary Islands, Spain). Environmental Research, 109, 607-613. http://dx.doi.org/10.1016/j.envres.2009.03.008
- Engel, S.M., Levy, B., Liu, Z., Kaplan, D. and Wolff, M.S. (2006) Xenobiotic Phenols in Early Pregnancy Amniotic Fluid. Reproductive Toxicology, 21, 110-112. http://dx.doi.org/10.1016/j.reprotox.2005.07.007
- Silva, M.J., Reidy, J.A., Herbert, A.R., Preau Jr., J.L. and Needham, L.L. (2004) Detection of Phthalate Metabolites in Human Amniotic Fluid. Bulletin of Environmental Contamination and Toxicology, 72, 1226-1231. http://dx.doi.org/10.1007/s00128-004-0374-4
- Wittassek, M., Angerer, J., Koloss-Gehring, M., Schafer, S.D. and Klockenbusch, W. (2009) Fetal Exposure to Phthalates—A Pilot Study. International Journal of Hygiene and Environmental Health, 212, 492-498. http://dx.doi.org/10.1016/j.ijheh.2009.04.001
- Huang, P.C., Kuo, P.L., Chou, Y.Y., Lin, S.J. and Lee, C.C. (2009) Association between Prenatal Exposure to Phthalates and the Health of Newborns. Environment International, 35, 14-20. http://dx.doi.org/10.1016/j.envint.2008.05.012
- Swan, S.H., Liu, F., Hines, M., Kruse, R.L. and Wang, C. (2009) Prenatal Phthalate Exposure and Reduced Masculine Play in Boys. International Journal of Andrology, 32, 1-9.
- Dobek, D., Karmowski, A., Sobiech, K.A., Terpilowski, L. and Mis-Michalek, M. (1998) Average Quantitative Concentration of Cotinine within the System Pregnant Woman-Baby. Archivum Immunologiae et Therapia Experimentalis (Warsz), 46, 59-61.