Bio-Chemical Analysis and FTIR-Spectral Studies of Artificially Removed Renal Stone Mineral Constituents
- 1 Department of Bio-chemistry, Avinashilingam University for Women, Coimbatore, India.
- 2 Department of Physics, Thiruvalluvar Govt. Arts College, Rasipuram, Namakkal,India-637401
- 3 Department of Bio-chemistry, Avinashilingam University for Women, Coimbatore, India.
Abstract
The kidneys are the vital organs of the human body. Its function is a peculiar one and it continues till end of the life. The kidneys are largely responsible for the regulation of water, electrolyte and acid-base balance in the body. They excrete metabolic waste products such as urea, creatinine, creatine, uric acid, sulphate and phosphate from the body. The kidneys reabsorb and retain several substances of biochemical importance in the body. If the mineral content of the blood increases, mineral deposition starts (crystal growth) automatically in kidney or in any part of the urinary tracts leading to the formation of renal stones. In the present research, five renal stones were collected from the affected people by lithotropic treatment method. The FTIR spectrum was taken for all the stones. From the spectrum, the chemical constituents of renal stones were identified and compared with the reported values. Finally FTIR spectral results were compared with the biochemical analysis result to confirm the chemical constituents present in the renal stones.
- Mandel N. (1986), ‘Urinary tract calculi’, Lab. Med., Vol.17, pp.449-458.
- Mitcheson H.D., Zamenhof R.G., Bankoff M.S. and Prien E.L. (1983), ‘Determination of the chemical composition of urinary calculi by computerized tomography’, J. Urol., Vol.130, pp.814-819.
- Sutor D.J. and Scheidt S. (1968), ‘Identification standards for human urinary calculus components using crystallographic methods’, Br. J. Urol., Vol.40, pp22-28.
- Alexander Randall, (1942), ‘Analysis of urinary calculi through the use of the polarizing microscope,’ J.Urol., Vol.48, pp.642-649.
- Hesse A. and Bach D., (1982), ‘Stone analysis by infrared spectroscopy’. In. Urinary Stones: Clinical and Laboratory Aspects, Ed. Alan Rose G., University Park Press, Baltimore, pp.165-171.
- Berthelot M., Cornu G., Daudon M., Helbert M. and Laurence C. (1987), ‘Computer- aided infrared analysis of urinary calculi,’ Clin. Chem., Vol.33, pp.2070-2073.
- Leemann C.A., McClure G.L.and Smdens I. (1988), ‘Identification of renal calculi by computerized infrared spectroscopy’, Clin. Chem. Acta., Vol.173, pp.107-116.
- Venkata Ramana Kodati, Tomasi G.E., Turumin J.L. and Anthony T.Tu (1990), ‘Raman spectroscopic identification of calium oxalate type kidney stone’, Applied Spectroscopy, Vol.44, pp.1408-1411.
- Alan Rose G. and Woodfine C., (1976) ‘The Thermogravimetric analysis of renal stones(in clinical practice)’ Br.J.Urol., Vol.48, pp.403-412.
- Beeler M.F., Veith D.A., Morriss R.H., and Biskind G.R. (1964), ‘Analysis of Urinary Calculus, Comparision of Methods,’ Amer. J. Clin. Path., Vol.41, pp.553-560.
- Yean-Chin Tsay (1961), ‘Application of infrared spectroscopy to analysis of urinary calculi’, J. Urol., Vol.86, pp.838-854.
- Hesse A. and Siener R. (1997), ‘Current aspects of epidemiology and nutrition in urinary stone disease’, World J. Urol., Vol.15, pp.165-171
- Juana Bellanto (1990), ‘Infrared spectroscopy of urinary calculi’. In. Renal Tract Stone: Metabolic Basis and Clinical Practise, Ed. Wickham J.E.A. and Colinbuck, A., Churchill Livingstone, Newyork, pp. 45-57.
- Sundaramoorthi.P, Kalainathan.S, (2007) Growth and characterizations studies of SMHP single crystal in silica gel medium and laser induced nucleation reduction process. J. Bio. Chem. Engg., 34 pp 244-249.