Improvement of Renewable Bioenergy Production in Microbial Fuel Cells with Saponin Supplementation
- 1 Horace Greeley High School, Chappaqua, NY, USA
- 2 Deerfield Academy, Deerfield, MA, USA
- 3 Oakton High School, Vienna, VA USA
- 4 School of Arts & Sciences, University of Virginia, Charlottesville, VA, USA
- 5 School of Arts & Sciences, Emory University, Atlanta, GA, USA
- 6 Bachelor of Science, Villanova University, Villanova, PA, USA
- 7 College of Letters & Sciences, University of California Los Angeles, Los Angeles, CA, USA
- 8 School of Arts & Sciences, University of Michigan, Ann Arbor, MI, USA
- 9 Division of Biological Sciences, Revelle College, University of California, San Diego, CA, USA
- 10 Fuzbien Technology Institute, Rockville, MD, USA
Abstract
Microbial fuel cell (MFC) is one of renewable biofuel production technology that directly converts biomass to electricity. Cellulosic biomass is particularly attractive renewable resources for its low cost and abundance and neutral carbon balance. However, methanogenesis remains as a major factor limiting MFC performance. The current study reports that saponin addition at 0.05 % w/v dose to anolyte in MFCs inhibited methanogenesis and improves power generation and cellulose fermentation. Mediator-less two chamber H-type MFCs were prepared using rumen fluid as anode inocula at 20 % v/v of anolyte to convert finely ground pine tree (Avicel) at 2 % , w/v to electricity. Saponin was added to the anode of MFC at 0.005 % or 0.05 % v/v dosage for treatment. MFC power and current across an external resistor were measured daily for 10d. On d10, collected gases from anode compartment were measured for total gas volume and analyzed for gas composition on gas chromatography. Supplementation of saponin to MFC at 0.005 % did not have any effects on electricity generation or biogas production and composition. Saponin at 0.05% dose reduced 10 % of methane production and increased 40 % of CO 2 production and 6.4 % of total gas production for 10d MFC operation. Voltage across resistor prior to treatment addition (d0) was 164.75 ± 9.07 mV. In control group, voltage across resistor did not change (P = 0.9153) with time course and mean was 167.8 ± 8.20 mV ranged from 157 to 174.5 mV during 10d operation. In 0.05 % Saponin group, voltage across resistor increased (P < 0 .0001) after d2 and mean was 187.3 ± 4.30 mV ranged between 161.5 and 204.0 mV and the 10d mean of voltage across resistor in 0.05 % Saponin was greater (P < 0 .0001) than in control group. 0.05 % Saponin also had greater voltage across resistor at d5 (P = 0.0030) and d6 (P = 0.0246) than control. End point potential increased (P < 0 .0001) in 0.05 % Saponin after d2. 0.05 % Saponin had greater (P < 0.05) end point potentials than control at d1, d4, d7, d10, and also 10d mean was greater (731.9 vs 606.5 mV; P < 0 .0001) in 0.05 % Saponin. Power density increased (P < 0 .0001) after d2 in 0.05 % Saponin. 0.05 % Saponin MFCs had greater (P < 0.05) power density than control at d5 and d6, and also a greater (P < 0 .0001) overall mean of 10d operation. The current study provides strong background for potential use of saponin and saponin containing natural resources for methanogenesis inhibitor and cellulolysis enhancer in MFC and also cellulolysis reactors.
- Goldemberg, J. and Johansson, T.B. (2004) World Energy Assessment Overview: 2004 Update. United Nations Development Programme, New York.
- EPA (2018) Inventory of U.S. Greenhouse Gas Emissions and Sinks: 1990-2018. https://www.epa.gov/ghgemissions/inventory-us-greenhouse-gas-emissions-and-sinks-1990-2018
- Saratale, G. and Oh, S. (2011) Production of Thermotolerant and Alkalotolerant Cellulolytic Enzymes by Isolated Nocardiopsis sp. KNU. Biodegradation, 22, 905-919. https://doi.org/10.1007/s10532-010-9450-0
- Levin, D.B., Islam, R., Cicek, N. and Sparling, R. (2006) Hydrogen Production by Clostridium Thermocellum 27405 from Cellulosic Biomass Substrates. International Journal of Hydrogen Energy, 31, 1496-1503. https://doi.org/10.1016/j.ijhydene.2006.06.015
- Logan, B.E. and Regan, J.M. (2006) Microbial Fuel Cells—Challenges and Applications. Environmental Science & Technology, 40, 5172-5180. https://doi.org/10.1021/es0627592
- Bond, D.R. and Lovley, D.R. (2003) Electricity Production by Geobacter sulfurreducens Attached to Electrodes. Applied and Environmental Microbiology, 69, 1548-1555. https://doi.org/10.1128/AEM.69.3.1548-1555.2003
- Liu, H., Cheng, S. and Logan, B.E. (2005) Production of Electricity from Acetate or Butyrate Using a Single-Chamber Microbial Fuel Cell. Environmental Science & Technology, 39, 658-662. https://doi.org/10.1021/es048927c
- Min, B., Kim, J., Oh, S., Regan, J.M. and Logan, B.E. (2005) Electricity Generation from Swine Wastewater Using Microbial Fuel Cells. Water Research, 39, 4961-4968. https://doi.org/10.1016/j.watres.2005.09.039
- Aelterman, P., Rabaey, K., Clauwaert, P. and Verstraete, W. (2006) Microbial Fuel Cells for Wastewater Treatment. Water Science and Technology, 54, 9-15. https://doi.org/10.2166/wst.2006.702
- Hassan, S.H., Kim, Y.S. and Oh, S.E. (2012) Power Generation from Cellulose Using Mixed and Pure Cultures of Cellulose-Degrading Bacteria in a Microbial Fuel Cell. Enzyme and Microbial Technology, 51, 269-273. https://doi.org/10.1016/j.enzmictec.2012.07.008
- Ishii, S., Suzuki, S., Norden-Krichmar, T.M., Nealson, K.H., Sekiguchi, Y. and Gorby, Y.A. (2012) Functionally Stable and Phylogenetically Diverse Microbial Enrichments from Microbial Fuel Cells during Wastewater Treatment. PLoS ONE, 7, e30495. https://doi.org/10.1371/journal.pone.0030495
- Lynd, L.R., Weimer, P.J., van Zyl, W.H. and Pretorius, I.S. (2002) Microbial Cellulose Utilization: Fundamentals and Biotechnology. Microbiology and Molecular Biology Reviews, 66, 506-577. https://doi.org/10.1128/MMBR.66.3.506-577.2002