Development of a Consolidated Anaerobic Digester and Microbial Fuel Cell to Produce Biomethane and Electricity from Cellulosic Biomass Using Bovine Rumen Microorganisms — Oak Academic Publishing
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Development of a Consolidated Anaerobic Digester and Microbial Fuel Cell to Produce Biomethane and Electricity from Cellulosic Biomass Using Bovine Rumen Microorganisms
Centreville High School, Clifton, VA, USA
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West Springfield High School, Springfield, VA, USA
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Centreville High School, Clifton, VA, USA
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Department of Biological Science, University of Southern California, Los Angeles, CA, USA
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Biology, College of Arts & Science, Cornell University, Ithaca, NY, USA
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Biological Science, Mellon College of Science, Carnegie Mellon University, Pittsburgh, PA, USA
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Biological Science, Mellon College of Science, Carnegie Mellon University, Pittsburgh, PA, USA
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Department of Biology, Amherst College, Amherst, MA, USA
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Biology, Science Program, University of Alberta, Edmonton, Canada
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Fuzbien Technology Institute (FTI), Rockville, USA
1 Centreville High School, Clifton, VA, USA
2 West Springfield High School, Springfield, VA, USA
3 Centreville High School, Clifton, VA, USA
4 Department of Biological Science, University of Southern California, Los Angeles, CA, USA
5 Biology, College of Arts & Science, Cornell University, Ithaca, NY, USA
6 Biological Science, Mellon College of Science, Carnegie Mellon University, Pittsburgh, PA, USA
7 Biological Science, Mellon College of Science, Carnegie Mellon University, Pittsburgh, PA, USA
8 Department of Biology, Amherst College, Amherst, MA, USA
9 Biology, Science Program, University of Alberta, Edmonton, Canada
10 Fuzbien Technology Institute (FTI), Rockville, USA
Microbial fuel cells (MFCs) are bioelectrochemical systems that convert chemical energy contained in organic matter into electrical energy by using the catalytic (metabolic) activity of living microorganisms. Mediator-less two chamber H-type MFCs were constructed in the current study, using dairy digester microbial population as anode inocula to convert finely ground pine tree (Avicel) at 2% (w/v) to electricity. MFCs were placed at 37 ° C and after the circuit voltage was stabilized on d9, bovine rumen microorganisms cultured anaerobically for 48 hrs in cellulose broth media were added to treatment group of MFC at 1% v/v dosage. MFC power and current across an external resistor were measured daily for 10 d. At the end of incubation on d19 head space gas and anode chamber liquid solutions were collected and analyzed for total gas volume and composition, and volatile fatty acids, respectively. Addition of enriched rumen microorganisms to anaerobic anode chamber increased cellulose digestibility and increased both CO 2 and methane production; however, it decreased the methane to CO 2 ratio. Over the experimental period, electricity generation was increased with rumen microorganism addition, and power density normalized to anode surface area was 17.6 to 67.2 mW/m 2 with average of 36.0 mW/m 2 in treatment, while control group had 3.6 to 21.6 (AVE 12.0) mW/m 2 . These observations imply that biocatalysis in MFCs requires additional cellulolytic activities to utilize structural biomass in bioenergy production.
Goldemberg, J. and Johansson, T.B. (2004) World Energy Assessment Overview: 2004 Update. United Nations Development Programme, New York.
Saratale, G. and Oh, S. (2011) Production of Thermotolerant and Alkalotolerant Cellulolytic Enzymes by Isolated Nocardiopsis sp. KNU. Biodegradation, 22, 905-919. https://doi.org/10.1007/s10532-010-9450-0
Lashof, D.A. and Ahuja, D.R. (1990) Relative Contributions of Greenhouse Gas Emissions to Global Warming. Nature, 344, 529-531. https://doi.org/10.1038/344529a0
Gong, M., Liu, X., Trembly, J. and Johnson, C. (2007) Sulfur-Tolerant Anode Materials for Solid Oxide Fuel Cell Application. Journal of Power Sources, 168, 289-298.
Kim, I.S., Chae, K.J., Choi, M.J. and Verstraete, W. (2008) Microbial Fuel Cells: Recent Advances, Bacterial Communities and Application beyond Electricity Generation. Environmental Engineering Research, 13, 51-65.
Levin, D.B., Islam, R., Cicek, N. and Sparling, R. (2006) Hydrogen Production by Clostridium Thermocellum 27405 from Cellulosic Biomass Substrates. International Journal of Hydrogen Energy, 31, 1496-1503. https://doi.org/10.1016/j.ijhydene.2006.06.015
Rismani-Yazdi, H., Christy, A.D., Dehority, B.A., Morrison, M., Yu, Z. and Tuovinen, O.H. (2007) Electricity Generation from Cellulose by Rumen Microorganisms in Microbial Fuel Cells. Biotechnology and Bioengineering, 97, 1398-1407. https://doi.org/10.1002/bit.21366
Perlack, R., Wright, L.L., Turhollow, A.F., Graham, A.F., Stokes, B.J. and Erbach, D.C. (2005) Biomass as Feedstock for a Bioenergy and Bioproducts Industry: The Technical Feasibility of a Billion-Ton Annual Supply. Oak Ridge National Laboratory, Oak Ridge, TN.
Niessen, J., Schroder, U., Harnisch, F. and Scholz, F. (2005) Gaining Electricity from in situ Oxidation of Hydrogen Produced by Fermentative Cellulose Degradation. Letters in Applied Microbiology, 41, 286-290. https://doi.org/10.1111/j.1472-765X.2005.01742.x
Howard, R.L., Abotsi, E., Jansen van Rensburg, E.L. and Howard, S. (2003) Lignocellulose Biotechnology: Issues of Bioconversion and Enzyme Production. African Journal of Biotechnology, 2, 602-619. https://doi.org/10.5897/AJB2003.000-1115
Palmqvist, E. and Hahn-Hagerdal, B. (2000) Fermentation of Lignocellulosic Hydrolysates. I: Inhibition and Detoxification. Bioresource Technology, 74, 17-24.
Mielenz, J.R. (2001) Ethanol Production from Biomass: Technology and Commercialization Status. Current Opinion in Microbiology, 4, 324-329. https://doi.org/10.1016/S1369-5274(00)00211-3
Powlson, D.S., Riche, A.B. and Shield, I. (2005) Biofuels and Other Approaches for Decreasing Fossil Fuel Emissions from Agriculture. Annals of Applied Biology, 146, 193-201. https://doi.org/10.1111/j.1744-7348.2005.040056.x
Ni, M., Leung, D.Y.C., Leung, M.K.H. and Sumathy, K. (2006) An Overview of Hydrogen Production from Biomass. Fuel Processing Technology, 87, 461-472. https://doi.org/10.1016/j.fuproc.2005.11.003
Lo, K., Liao, P. and Gao, Y. (1994) Anaerobic Treatment of Swine Wastewater Using Hybrid UASB Reactors. Bioresource Technology, 47, 153-157.
Parawira, W., Read, J.S., Mattiasson, B. and Bjornsson, L. (2008) Energy Production from Agricultural Residues: High Methane Yields in Pilot-Scale Two-Stage Anaerobic Digestion. Biomass Bioenergy, 32, 44-50. https://doi.org/10.1016/j.biombioe.2007.06.003
Sakar, S., Yetilmezsoy, K. and Kocak, E. (2009) Anaerobic Digestion Technology in Poultry and Livestock Waste Treatment—A Literature Review. Waste Management & Research, 27, 3-18. https://doi.org/10.1177/0734242X07079060
Premier, G.C., Kim, J.R., Massanet-Nicolau, J., Kyazze, G., Esteves, S.R.R., Penumathsa, B.K.V., Rodríguez, J., Maddy, J., Dinsdale, R.M. and Guwy, A.J. (2013) Integration of Biohydrogen, Biomethane and Bioelectrochemical Systems. Renewable Energy, 49, 188-192. https://doi.org/10.1016/j.renene.2012.01.035
Logan, B.E. and Regan, J.M. (2006) Microbial Fuel Cells-Challenges and Applications. Environmental Science & Technology, 40, 5172-5180. https://doi.org/10.1021/es0627592
EPA (2016) Inventory of U.S. Greenhouse Gas Emissions and Sinks: 1990-2014. https://www.epa.gov/sites/production/files/2016-04/documents/us-ghg-inventory-2016-main-text.pdf
Fradler, K.R., Kim, J.R., Shipley, G., Massanet-Nicolau, J., Dinsdale, R.M., Guwy, A.J. and Premier, G.C. (2014) Operation of a Bioelectrochemical System as a Polishing Stage for the Effluent from a Two-Stage Biohydrogen and Biomethane Production Process. Biochemical Engineering Journal, 85, 125-131. https://doi.org/10.1016/j.bej.2014.02.008
Kim, T., An, J., Jang, J.K. and Chang, I.S. (2015) Coupling of Anaerobic Digester and Microbial Fuel Cell for COD Removal and Ammonia Recovery. Bioresource Technology, 195, 217-222. https://doi.org/10.1016/j.biortech.2015.06.009
Xie, B., Liu, B., Yi, Y., Yang, L., Liang, D., Zhu, Y. and Liu, H. (2016) Microbiological Mechanism of the Improved Nitrogen and Phosphorus Removal by Embedding Microbial Fuel Cell in Anaerobic-Anoxic-Oxic Wastewater Treatment Process. Bioresource Technology, 207, 109-117. https://doi.org/10.1016/j.biortech.2016.01.090
Ishii, S., Suzuki, S., Norden-Krichmar, T.M., Nealson, K.H., Sekiguchi, Y. and Gorby, Y.A. (2012) Functionally Stable and Phylogenetically Diverse Microbial Enrichments from Microbial Fuel Cells during Wastewater Treatment. PLoS ONE, 7, e30495. https://doi.org/10.1371/journal.pone.0030495
Desvaux, M., Guedon, E. and Petitdemange, H. (2000) Cellulose Catabolism by Clostridium Cellulolyticum Growing in Batch Culture on Defined Medium. Applied and Environmental Microbiology, 66, 2461-2470. https://doi.org/10.1128/AEM.66.6.2461-2470.2000
Krause, D.O., Denman, S.E., Mackie, R.I. and Morrison, M. (2003) Opportunities to Improve Fiber Degradation in the Rumen: Microbiology, Ecology, and Genomics. FEMS Microbiology Reviews, 27, 663-693. https://doi.org/10.1016/S0168-6445(03)00072-X
Chung, R., Moon, D., Chang, Y., Chung, D., Kong, T. and Kim, J. (2018) The Cellulolytic Bacteria R. albus for Improving the Efficiency of Microbial Fuel Cell. Journal of Sustainable Bioenergy Systems, 8, 36-46. https://doi.org/10.4236/jsbs.2018.82003
Schwarz, W.H. (2001) The Cellulosome and Cellulose Degradation by Anaerobic Bacteria. Applied Microbiology and Biotechnology, 56, 634-649. https://doi.org/10.1007/s002530100710
Leschine, S.B. (1995) Cellulose Degradation in Anaerobic Environments. Annual Review of Microbiology, 49, 399-426. https://doi.org/10.1146/annurev.mi.49.100195.002151
Freguia, S., The, E.H., Boon, N., Leung, K.M., Keller, J. and Rabaey, K. (2010) Microbial Fuel Cells Operating on Mixed Fatty Acids. Bioresource Technology, 101, 1233-1238. https://doi.org/10.1016/j.biortech.2009.09.054
31-Wang, Z.Q., Hong, M., Shu, Y., Chen, Y., Dong, L.X. and Ming, Y. (2014) Rumen Bacteria Convert Cellulose into Electricity in Two-Chamber Microbial Fuel Cell. Journal of Chemical and Pharmaceutical Research, 6, 727-732.
Choi, S. (2015) Microscale Microbial Fuel Cells: Advances and Challenges. Biosensors Bioelectronics, 69, 8-25.