Isolation of Picocyanobacteria (Order <i>Synechococcales</i>) and Occurrence of the Cyanotoxin Anatoxin-A in a Shallow Mesotrophic Pond — Oak Academic Publishing
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Isolation of Picocyanobacteria (Order <i>Synechococcales</i>) and Occurrence of the Cyanotoxin Anatoxin-A in a Shallow Mesotrophic Pond
Lim-Tex, North Andover, Massachusetts, USA
,
Brewster Natural Resources Advisory Commission, Brewster, Massachusetts, USA
,
Brewster Ponds Coalition, Brewster, Massachusetts, USA
,
Department of Civil and Environmental Engineering, University of New Hampshire, College of Engineering and Physical Sciences, Durham, New Hampshire, USA
,
Department of Civil and Environmental Engineering, University of New Hampshire, College of Engineering and Physical Sciences, Durham, New Hampshire, USA
,
Department of Biological Sciences, University of New Hampshire, Center for Freshwater Biology and Ecotoxicology, Durham, New Hampshire, USA
1 Lim-Tex, North Andover, Massachusetts, USA
2 Brewster Natural Resources Advisory Commission, Brewster, Massachusetts, USA
3 Brewster Ponds Coalition, Brewster, Massachusetts, USA
4 Department of Civil and Environmental Engineering, University of New Hampshire, College of Engineering and Physical Sciences, Durham, New Hampshire, USA
5 Department of Civil and Environmental Engineering, University of New Hampshire, College of Engineering and Physical Sciences, Durham, New Hampshire, USA
6 Department of Biological Sciences, University of New Hampshire, Center for Freshwater Biology and Ecotoxicology, Durham, New Hampshire, USA
We have verified the use of a serial filtration method to isolate picocyanobacteria for analysis. We used eDNA metabarcoding to confirm the picocyanobacteria as members of the Order Synechococcales , Genus Cyanobium , specifically Cyanobium 6307. Fluorometric analysis using accessory pigments phycocyanin and phycoerythrin described periods of excess biomass, where the net growth rate model confirmed these conditions. The total anatoxin-a concentrations in the picocyanobacterial sample ranged from 0.0074 - 6.41 μg · L -1 representing a 40-fold difference over the entire sampling season. Sampling frequency of every three days appeared to be an important factor in capturing these changes in anatoxin-a concentration. During a period of excess biomass, we were able to establish a linear correlation between cyanobacterial biomass and Anatoxin-a concentrations.
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