Isolation of Picocyanobacteria (Order <i>Synechococcales</i>) and Occurrence of Cyanotoxins (Anatoxin-a) in Saline Microhabitats at Martha’s Vineyard, MA
- 1 Lim-Tex, North Andover, Massachusetts, USA
- 2 Martha’s Vineyard Commission, Oak Bluffs, Massachusetts, USA
- 3 Martha’s Vineyard Commission, Oak Bluffs, Massachusetts, USA
- 4 College of Engineering and Physical Sciences, University of New Hampshire, Durham, USA
- 5 College of Engineering and Physical Sciences, University of New Hampshire, Durham, USA
- 6 Center for Freshwater Biology and Ecotoxicology, University of New Hampshire, Durham, USA
Abstract
We have used serial filtration to isolate picocyanobacteria from brackish and marine microhabitats for analysis. We used 16s metabarcoding to confirm the picocyanobacteria as members of the Order Synechococcales , Genus Cyanobium 6307 (Upper Chilmark Pond) and differing abundances of Cyanobium 6307 and Synechococcus 9902 (Chilmark Pond, Edgartown Great Pond, Tisbury Great Pond and Tashmoo Pond). The proportion and composition of (pico)cyanobacteria in water samples were influenced by the salinity concentrations at various sites, as evidenced by fluorometry and 16s metabarcoding analysis. The cyanobacterial neurotoxin anatoxin-a was present in the picocyanobacterial samples from all studied sites. Additional analyses using fluorometry and 16s metabarcoding described members of the Order Nostocales , including a halotolerant population of Dolichospermum sp., Sphaerospermopsis spp. and Nodularia spp. in Upper Chilmark Pond. We were able to establish a positive linear correlation between cyanobacterial biomass (phycocyanin) and anatoxin-a concentrations using samples taken from Upper Chilmark Pond.
- Cabello-Yeves, P.J., Callieri, C., Picazo, A., Schallenberg, L., Huber, P., Roda-Garcia, J.J., Bartosiewicz, M., Belykh, O.I., Tikhonova, I.V., Requena-Torcells, A., De Prado, P.M., Puxty, R.J., Millard, A.D., Camacho, A., Rodriguez-Valera, F. and Scanlan, D.J. (2022) Elucidating the Picocyanobacteria Salinity Divide through Ecogenomics of New Freshwater Isolates. BMC Biology, 20, Article No. 175. https://doi.org/10.1186/s12915-022-01379-z
- Hunter-Cevera, K.R., Post, A.F., Peacock E.E. and Sosik, H.M. (2016) Diversity of Synechococcus at the Martha’s Vineyard Coastal Observatory: Insights from Culture Isolations, Clone Libraries and Flow Cytometry. Microbial Ecology, 71, 276-289. https://doi.org/10.1007/s00248-015-0644-1
- Leland, N.J., Haney, J.F., Conte, K., Malkus-Benjamin, K. and Horsley, B. (2019) Evaluation of Size Structure in Freshwater Cyanobacterial Populations: Methods to Quantify Risk Associated with Changes in Biomass and Microcystin Concentrations. Journal of Water Resource and Protection, 11, 810-829. https://doi.org/10.4236/jwarp.2019.116049
- Jakubowska, N. and Szelag-Wasielewska, E., (2015) Toxic Picoplanktonic Cyanobacteria—Review. Marine Drugs, 13, 1497-1518. https://doi.org/10.3390/md13031497
- Chapman, A.D. and Foss, A. (2019) GreenWater Laboratories Potentially Toxigenic (PTOX) Cyanobacteria List. GreenWater Laboratories, Palatka.
- Gin, K.Y-H., Sim, Z.Y., Goh, K.C., Kok, J.W.K., Te, S.H., Tran, N.H., Li, W. and He, Y. (2021) Novel Cyanotoxin-Producing Synechococcus in Tropical Lakes. Water Research, 192, Article ID: 116828. https://doi.org/10.1016/j.watres.2021.116828
- Leland, N.J., Pearson, K.C., Burke, M., Miller, J.T. Watts, A. and Haney, J.F. (2023) Isolation of Picocyanobacteria (Order Synechococcales) and Occurrence of the Cyanotoxin Anatoxin-A in a Shallow Mesotrophic Pond. Journal of Water Resource and Protection, 15, 299-314. https://doi.org/10.4236/jwarp.2023.156017
- Amiscience Corporation (2022) Certificate of Calibration, Phycocyanin and Phycoerythrin Fluorometer. http://www.amiscience.com/
- Gold Standard Diagnostics, Anatoxin-A(VFDF) Product # 520060 Warminster, PA. https://www.goldstandarddiagnostics.us/
- Parada, A.E., Needham, D.M. and Fuhrman, J.A. (2016) Every Base Matters: Assessing Small Subunit rRNA Primers for Marine Microbiomes with Mock Communities, Time Series and Global Field Samples. Environmental Microbiology, 18, 1403-1414. https://doi.org/10.1111/1462-2920.13023