A mesocosm-based study was conducted to assess the effect of glucose and hydrogen peroxide on periphyton communities. These chemicals have been found to be effective at controlling cyanobacteria blooms in the water column but their impact on attached communities is unknown. The experimental design included a total of 4 treatments: control (no chemicals; 3 replicates); hydrogen peroxide (3 replicates); glucose alone (3 different concentrations [no replicates]); and additive glucose (3 replicates). After 34 days, mean values of chlorophyll a were lower in all experimental treatments compared to the control; mean AFDM values were lower in all treatments except the unreplicated high glucose alone treatment. In contrast, mean autotrophic index values (AFDM/chlorophyll a ) were greater in all treatments compared to the control, indicating heterotrophs were more resistant to the chemical treatments than autotrophs. Periphyton community biodiversity was much lower in the additive glucose and moderate glucose alone treatments than the hydrogen peroxide and control treatments. The relative abundance of the bacteria Asticcacaul is and Sphingorhabdus responded positively to the glucose treatments, whereas relative abundance of Nevskia and Caenimonas declined in both the hydrogen peroxide and glucose treatments. In terms of relative abundance, no cyanobacteria taxa were detected among the top 20 taxa. We conclude that the autotrophic component of periphyton communities is especially vulnerable to hydrogen peroxide and glucose treatments, and that any management strategy employing these chemicals should be aware of this potential impact.
Vadeboncoeur, Y. and Steinman, A.D. (2002) Periphyton Function in Lake Ecosystems. The Scientific World Journal , 2, 1449-1468. https://doi.org/10.1100/tsw.2002.294
Vadeboncoeur, Y., Peterson, G., Vander Zanden, M.J. and Kalff, J. (2008) Benthic Algal Production across Lake Size Gradients: Interactions among Morphometry, Nutrients, and Light. Ecology , 89, 2542-2552. https://doi.org/10.1890/07-1058.1
Vesterinen, J., Devlin, S.P., Syväranta, J. and Jones, R.I. (2017) Influence of Littoral Periphyton on Whole‐Lake Metabolism Relates to Littoral Vegetation in Humic Lakes. Ecology , 98, 3074-3085. https://doi.org/10.1002/ecy.2012
Gushulak, C.A.C., Haig, H.A., Kingsbury, M.V., Wissel, B., Cumming, B.F. and Leavitt, P.R. (2021) Effects of Spatial Variation in Benthic Phototrophs along a Depth Gradient on Assessments of Whole‐Lake Processes. Freshwater Biology , 66, 2118-2132. https://doi.org/10.1111/fwb.13820
Wilkinson, G.M., Walter, J.A., Buelo, C.D. and Pace, M.L. (2021) No Evidence of Widespread Algal Bloom Intensification in Hundreds of Lakes. Frontiers in Ecology and the Environment , 20, 16-21. https://doi.org/10.1002/fee.2421
Hou, X., Feng, L., Dai, Y., Hu, C., Gibson, L., Tang, J., et al . (2022) Global Mapping Reveals Increase in Lacustrine Algal Blooms over the Past Decade. Nature Geoscience , 15, 130-134. https://doi.org/10.1038/s41561-021-00887-x
Hallegraeff, G.M., Anderson, D.M., Belin, C., Bottein, M.D., Bresnan, E., Chinain, M., et al . (2021) Perceived Global Increase in Algal Blooms Is Attributable to Intensified Monitoring and Emerging Bloom Impacts. Communications Earth & Environment , 2, Article ID: 117. https://doi.org/10.1038/s43247-021-00178-8
Jeppesen, E., Søndergaard, M., Jensen, J.P., Havens, K.E., Anneville, O., Carvalho, L., et al . (2005) Lake Responses to Reduced Nutrient Loading—An Analysis of Contemporary Long‐Term Data from 35 Case Studies. Freshwater Biology , 50, 1747-1771. https://doi.org/10.1111/j.1365-2427.2005.01415.x
Beutel, M.W. and Horne, A.J. (1999) A Review of the Effects of Hypolimnetic Oxygenation on Lake and Reservoir Water Quality. Lake and Reservoir Management , 15, 285-297. https://doi.org/10.1080/07438149909354124
Jeppesen, E., Meerhoff, M., Jacobsen, B.A., Hansen, R.S., Søndergaard, M., Jensen, J.P., et al . (2007) Restoration of Shallow Lakes by Nutrient Control and Biomanipulation—The Successful Strategy Varies with Lake Size and Climate. Hydrobiologia , 581, 269-285. https://doi.org/10.1007/s10750-006-0507-3
Mucci, N., Dugheri, S., Bonari, A., Farioli, A., Rapisarda, V., Garzaro, G., et al . (2020) Health Risk Assessment Related to Hydrogen Peroxide Presence in the Workplace Atmosphere—Analytical Methods Evaluation for an Innovative Monitoring Protocol. International Journal of Occupational Medicine and Environmental Health , 33, 137-150. https://doi.org/10.13075/ijomeh.1896.01508
Kinley-Baird, C., Calomeni, A., Berthold, D.E., Lefler, F.W., Barbosa, M., Rodgers, J.H., et al . (2021) Laboratory-Scale Evaluation of Algaecide Effectiveness for Control of Microcystin-Producing Cyanobacteria from Lake Okeechobee, Florida (USA). Ecotoxicology and Environmental Safety , 207, Article ID: 111233. https://doi.org/10.1016/j.ecoenv.2020.111233
Mortimer, C.H. (1941) The Exchange of Dissolved Substances between Mud and Water in Lakes. The Journal of Ecology , 29, 280-329. https://doi.org/10.2307/2256395
Lürling, M., Smolders, A.J.P. and Douglas, G.D. (2020) Methods for the Management of Internal Phosphorus Loading in Lakes. In: Steinman, A.D. and Spears, B.M., Eds., Internal Phosphorus Loading of Lakes : Causes , Case Stud ies , and Management , J. Ross Publishing, 77-107.
Brand, L.E., Sunda, W.G. and Guillard, R.R.L. (1986) Reduction of Marine Phytoplankton Reproduction Rates by Copper and Cadmium. Journal of Experimental Ma rine Biology and Ecology , 96, 225-250. https://doi.org/10.1016/0022-0981(86)90205-4
Jančula, D. and Maršálek, B. (2011) Critical Review of Actually Available Chemical Compounds for Prevention and Management of Cyanobacterial Blooms. Chemosphere , 85, 1415-1422. https://doi.org/10.1016/j.chemosphere.2011.08.036
Barón, M., Arellano, J.B. and Gorgé, J.L. (1995) Copper and Photosystem II: A Controversial Relationship. Physiologia Plantarum , 94, 174-180. https://doi.org/10.1111/j.1399-3054.1995.tb00799.x
Bauzá, L., Aguilera, A., Echenique, R., Andrinolo, D. and Giannuzzi, L. (2014) Application of Hydrogen Peroxide to the Control of Eutrophic Lake Systems in Laboratory Assays. Toxins , 6, 2657-2675. https://doi.org/10.3390/toxins6092657
Lusty, M.W. and Gobler, C.J. (2020) The Efficacy of Hydrogen Peroxide in Mitigating Cyanobacterial Blooms and Altering Microbial Communities across Four Lakes in NY, Usa. Toxins , 12, Article No. 428. https://doi.org/10.3390/toxins12070428
Santos, A.A., Guedes, D.O., Barros, M.U.G., Oliveira, S., Pacheco, A.B.F., Azevedo, S.M.F.O., et al . (2021) Effect of Hydrogen Peroxide on Natural Phytoplankton and Bacterioplankton in a Drinking Water Reservoir: Mesocosm-Scale Study. Water Research , 197, Article ID: 117069. https://doi.org/10.1016/j.watres.2021.117069
Chen, Y., Zaman, F., Jia, Y., Huang, Y., Li, T., Bai, F., et al . (2024) Harmful Cyanobacterial Bloom Control with Hydrogen Peroxide: Mechanism, Affecting Factors, Development, and Prospects. Current Pollution Reports , 10, 566-579. https://doi.org/10.1007/s40726-024-00328-4
Barrington, D.J., Reichwaldt, E.S. and Ghadouani, A. (2013) The Use of Hydrogen Peroxide to Remove Cyanobacteria and Microcystins from Waste Stabilization Ponds and Hypereutrophic Systems. Ecological Engineering , 50, 86-94. https://doi.org/10.1016/j.ecoleng.2012.04.024
Vesper, S., Sienkiewicz, N., Struewing, I., Linz, D. and Lu, J. (2022) Prophylactic Addition of Glucose Suppresses Cyanobacterial Abundance in Lake Water. Life , 12, Article No. 385. https://doi.org/10.3390/life12030385
Linz, D., Struewing, I., Sienkiewicz, N., Steinman, A.D., Partridge, C.G., McIntosh, K., et al . (2024) Periodic Addition of Glucose Suppressed Cyanobacterial Abundance in Additive Lake Water Samples during the Entire Bloom Season. Journal of Water Resource and Protection , 16, 140-155. https://doi.org/10.4236/jwarp.2024.162009
Linz, D., Partridge, C.G., Hassett, M.C., Sienkiewicz, N., Tyrrell, K., Henderson, A., et al . (2024) Changes in Cyanobacterial Phytoplankton Communities in Lake-Water Mesocosms Treated with Either Glucose or Hydrogen Peroxide. Microorganisms , 12, Article No. 1925. https://doi.org/10.3390/microorganisms12091925
Cantonati, M. and Lowe, R.L. (2014) Lake Benthic Algae: Toward an Understanding of Their Ecology. Freshwater Science , 33, 475-486. https://doi.org/10.1086/676140
DeNicola, D.M. and Kelly, M. (2014) Role of Periphyton in Ecological Assessment of Lakes. Freshwater Science , 33, 619-638. https://doi.org/10.1086/676117
Feminella, J.W. and Hawkins, C.P. (1995) Interactions between Stream Herbivores and Periphyton: A Quantitative Analysis of Past Experiments. Journal of the North American Benthological Society , 14, 465-509. https://doi.org/10.2307/1467536
Tonkin, J.D., Death, R.G. and Barquín, J. (2014) Periphyton Control on Stream Invertebrate Diversity: Is Periphyton Architecture More Important than Biomass? Marine and Freshwater Research , 65, 818-829. https://doi.org/10.1071/mf13271
Dodds, W.K. (2003) The Role of Periphyton in Phosphorus Retention in Shallow Freshwater Aquatic Systems. Journal of Phycology , 39, 840-849. https://doi.org/10.1046/j.1529-8817.2003.02081.x
Larned, S.T., Nikora, V.I. and Biggs, B.J.F. (2004) Mass‐Transfer‐Limited Nitrogen and Phosphorus Uptake by Stream Periphyton: A Conceptual Model and Experimental Evidence. Limnology and Oceanography , 49, 1992-2000. https://doi.org/10.4319/lo.2004.49.6.1992
Pećić, M., Grašić, S., Gajić, D., Popović, S., Subakov Simić, G. and Predojević, D. (2023) Periphyton Efficiency in Phosphorus Accumulation Affected by Phytoplankton Dynamics in Reservoir for Water Supply. Ecological Engineering , 191, Article ID: 106963. https://doi.org/10.1016/j.ecoleng.2023.106963
Liboriussen, L. and Jeppesen, E. (2005) Structure, Biomass, Production and Depth Distribution of Periphyton on Artificial Substratum in Shallow Lakes with Contrasting Nutrient Concentrations. Freshwater Biology , 51, 95-109. https://doi.org/10.1111/j.1365-2427.2005.01481.x
Struewing, I., Sienkiewicz, N., Zhang, C., Dugan, N. and Lu, J. (2022) Effective Early Treatment of Microcystis Exponential Growth and Microcystin Production with Hydrogen Peroxide and Hydroxyapatite. Toxins , 15, Article No. 3. https://doi.org/10.3390/toxins15010003
APHA (American Public Health Association) (1999) Standard Methods for the Ex-amination of Water and Wastewater. 19th Edition, American Public Health Association.
Steinman, A.D., Lamberti, G.A., Leavitt, P.R. and Uzarski, D.G. (2017) Biomass and Pigments of Benthic Algae. In: Hauer, F.R. and Lamberti, G.A., Eds., Methods in Stream Ecology , Volume 1, Elsevier, 223-241. https://doi.org/10.1016/b978-0-12-416558-8.00012-3
Quast, C., Pruesse, E., Yilmaz, P., Gerken, J., Schweer, T., Yarza, P., et al . (2012) The SILVA Ribosomal RNA Gene Database Project: Improved Data Processing and Web-Based Tools. Nucleic Acids Research , 41, D590-D596. https://doi.org/10.1093/nar/gks1219
Lefler, F.W., Berthold, D.E. and Laughinghouse IV, H.D. (2023) Cyanoseq: A Database of Cyanobacterial 16S rRNA Gene Sequences with Curated Taxonomy. Journal of Phycology , 59, 470-480. https://doi.org/10.1111/jpy.13335
Davis, N.M., Proctor, D., Holmes, S.P., Relman, D.A. and Callahan, B.J. (2018) Simple Statistical Identification and Removal of Contaminant Sequences in Marker-Gene and Meta-Genomics Data.
R Core Team (2020) R: A Language and Environment for Statistical Computing. R Foundation for Statistical Computing. https://www.R-project.org/
Balaji-Prasath, B., Wang, Y., Su, Y.P., Hamilton, D.P., Lin, H., Zheng, L., et al . (2022) Methods to Control Harmful Algal Blooms: A Review. Environmental Chemistry Letters , 20, 3133-3152. https://doi.org/10.1007/s10311-022-01457-2
Pal, M., Yesankar, P.J., Dwivedi, A. and Qureshi, A. (2020) Biotic Control of Harmful Algal Blooms (HABs): A Brief Review. Journal of Environmental Management , 268, Article ID: 110687. https://doi.org/10.1016/j.jenvman.2020.110687
Zámocký, M., Gasselhuber, B., Furtmüller, P.G. and Obinger, C. (2012) Molecular Evolution of Hydrogen Peroxide Degrading Enzymes. Archives of Biochemistry and Biophysics , 525, 131-144. https://doi.org/10.1016/j.abb.2012.01.017
Lusty, M.W. and Gobler, C.J. (2023) Repeated Hydrogen Peroxide Dosing Briefly Reduces Cyanobacterial Blooms and Microcystin While Increasing Fecal Bacteria Indicators in a Eutrophic Pond. Journal of Environmental Sciences , 124, 522-543. https://doi.org/10.1016/j.jes.2021.11.031
Gao, L., Pan, X., Zhang, D., Mu, S., Lee, D. and Halik, U. (2015) Extracellular Polymeric Substances Buffer against the Biocidal Effect of H 2 O 2 on the Bloom-Forming Cyanobacterium Microcystis Aeruginosa. Water Research , 69, 51-58. https://doi.org/10.1016/j.watres.2014.10.060
Gao, X., Zheng, T., Yuan, X., Dong, Y. and Liu, C. (2023) Biocidal H 2 O 2 Treatment Emphasizes the Crucial Role of Cyanobacterial Extracellular Polysaccharides against External Strong Oxidative Stress. Environmental Science and Pollution Research , 30, 60654-60662. https://doi.org/10.1007/s11356-023-26840-6
Drake, W.M., Scott, J.T., Evans-White, M., Haggard, B., Sharpley, A., Rogers, C.W., et al . (2011) The Effect of Periphyton Stoichiometry and Light on Biological Phosphorus Immobilization and Release in Streams. Limnology , 13, 97-106. https://doi.org/10.1007/s10201-011-0359-z
Pladdies, T., Babenzien, H. and Cypionka, H. (2004) Distribution of Nevskia ramosa and Other Rosette-Forming Neustonic Bacteria. Microbial Ecology , 47, 218-223. https://doi.org/10.1007/s00248-003-1070-3
Stürmeyer, H., Overmann, J., Babenzien, H. and Cypionka, H. (1998) Ecophysiological and Phylogenetic Studies of Nevskia ramosa in Pure Culture. Applied and Environmental Microbiology , 64, 1890-1894. https://doi.org/10.1128/aem.64.5.1890-1894.1998
Le, V.V., Ko, S., Lee, S., Kang, M., Oh, H. and Ahn, C. (2022) Caenimonas aquaedulcis sp. nov., Isolated from Freshwater of Daechung Reservoir during Microcystis Bloom. Journal of Microbiology and Biotechnology , 32, 575-581. https://doi.org/10.4014/jmb.2201.01023
Vasilyeva, L.V., Omelchenko, M.V., Berestovskaya, Y.Y., Lysenko, A.M., Abraham, W., Dedysh, S.N., et al . (2006) Asticcacaulis benevestitus sp. nov., a Psychrotolerant, Dimorphic, Prosthecate Bacterium from Tundra Wetland Soil. International Journal of Systematic and Evolutionary Microbiology , 56, 2083-2088. https://doi.org/10.1099/ijs.0.64122-0
Glaeser, S.P. and Kämpfer, P. (2014) The Family Sphingomonadaceae. In: Rosenberg, E., DeLong, E.F., Lory, S., Stackebrandt, E. and Thompson, F., Eds., The Prokaryotes , Springer.
Jogler, M., Chen, H., Simon, J., Rohde, M., Busse, H., Klenk, H., et al . (2013) Description of Sphingorhabdus planktonica gen. nov., sp. nov. and Reclassification of Three Related Members of the Genus Sphingopyxis in the Genus Sphingorhabdus Gen. Nov. International Journal of Systematic and Evolutionary Microbiology , 63, 1342-1349. https://doi.org/10.1099/ijs.0.043133-0
Nold, S.C. and Zwart, G. (1998) Patterns and Governing Forces in Aquatic Microbial Communities. Aquatic Ecology , 32, 17-35. https://doi.org/10.1023/a:1009991918036
Stroud, J.T., Delory, B.M., Barnes, E.M., Chase, J.M., De Meester, L., Dieskau, J., et al . (2024) Priority Effects Transcend Scales and Disciplines in Biology. Trends in Ecology & Evolution , 39, 677-688. https://doi.org/10.1016/j.tree.2024.02.004
Sand‐Jensen, K. and Søndergaard, M. (1981) Phytoplankton and Epiphyte Development and Their Shading Effect on Submerged Macrophytes in Lakes of Different Nutrient Status. Internationale Revue der Gesamten Hydrobiologie und Hydrographie , 66, 529-552. https://doi.org/10.1002/iroh.19810660406
Vadeboncoeur, Y., Vander Zanden, M.J. and Lodge, D.M. (2002) Putting the Lake Back Together: Reintegrating Benthic Pathways into Lake Food Web Models. BioScience , 52, 44-54. https://doi.org/10.1641/0006-3568(2002)052[0044:ptlbtr]2.0.co;2
Lamberti, G.A. (1996) The Role of Periphyton in Benthic Food Webs. In: Jan Stevenson, R., et al ., Eds., Algal Ecology , Elsevier, 533-572. https://doi.org/10.1016/b978-012668450-6/50046-1