Groundwater remains a critical resource for rural communities in South Africa, yet its quality is questionable due to geogenic processes and anthropogenic activities. This research evaluated groundwater quality through hydrochemical and microbial analysis of 20 borehole samples used domestically in the rural area of Zebediela subregion, Limpopo Province, South Africa. Parameters analysed included major ions, trace metals and faecal indicator bacteria, with data interpreted using multivariate analyses including co-occurrence matrices, Pearson correlation heatmaps and Principal Component Analysis. Nitrate, sulphate, phosphate, chloride and fluoride were within permissible limits, but COD and BOD were slightly above recommended levels, indicating moderate ion concentrations. Microbial contamination was detected in 80% of the samples (including Heterotrophic, Escherichia coli , coliform, S almo nella species, Enterobacteriaceae , Bacillus cereus , S taphylococcus aureus and Enter ococci ), with Heterotrophic bacteria present in most samples, indicating emerging contamination. PCA identified mineral dissolution, evaporative concentration, organic pollution and redox-mediated metal mobilization as the dominant processes shaping groundwater chemistry in Zebediela subregion. Therefore, regular monitoring, improved sanitation infrastructure and protection of borehole surroundings are recommended to manage water security in the Zebediela sub-region.
KeywordsWater QualityBoreholeHeavy MetalsMicrobial ContaminationZebedielaRural Water Safety
Baddianaah, I., Dongzagla, A. and Salifu, S.N. (2024) Navigating Access to Safe Water by Rural Households in Sub-Saharan Africa: Insights from North-Western Ghana. Sustainable Environment , 10, Article ID: 2303803. https://doi.org/10.1080/27658511.2024.2303803
Ejiohuo, O., Onyeaka, H., Akinsemolu, A., Nwabor, O.F., Siyanbola, K.F., Tamasiga, P., et al . (2025) Ensuring Water Purity: Mitigating Environmental Risks and Safeguarding Human Health. Water Biology and Security , 4, Article ID: 100341. https://doi.org/10.1016/j.watbs.2024.100341
Tenebe, I.T., Babatunde, E.O., Ogarekpe, N.M., Emakhu, J., Etu, E., Edo, O.C., et al . (2024) Detection and Measurement of Bacterial Contaminants in Stored River Water Consumed in Ekpoma. Water , 16, Article No. 2696. https://doi.org/10.3390/w16182696
Tshona, S.S., Lungisa, S. and Mgweba, L. (2025) Thirsting for Solutions: Unpacking Inadequate Water Provision in Rural Communities. Africa’s Public Service Delivery and Performance Review , 13, a873. https://doi.org/10.4102/apsdpr.v13i1.873
Voudouris, K., Valipour, M., Kaiafa, A., Zheng, X.Y., Kumar, R., Zanier, K., et al . (2018) Evolution of Water Wells Focusing on Balkan and Asian Civilizations. Water Supply , 19, 347-364. https://doi.org/10.2166/ws.2018.114
Promilton, A.A.A., Ravindran, A.A., Pitchaimani, V.S., Kingston, J.V. and Karuppannan, S. (2025) Comprehensive Hydrogeochemical Characterization and Seasonal Water Quality Index Analysis for Sustainable Groundwater Management in Valliyur Region, Southern Tamil Nadu, India. Scientific Reports , 15, Article No. 33251. https://doi.org/10.1038/s41598-025-18285-3
Akhtar, N., Syakir Ishak, M.I., Bhawani, S.A. and Umar, K. (2021) Various Natural and Anthropogenic Factors Responsible for Water Quality Degradation: A Review. Water , 13, Article No. 2660. https://doi.org/10.3390/w13192660
Ravi, M., et al . (2025) Hydrogeochemical Evaluation and Groundwater Quality Assessment in Madurai South Taluk, Tamil Nadu, India. Journal of Environmental Studies , 39, 25-44. https://doi.org/10.21608/jesj.2025.390385.1130
Egbueri, J.C., Agbasi, J.C., Ayejoto, D.A., Khan, M.I. and Khan, M.Y.A. (2023) Extent of Anthropogenic Influence on Groundwater Quality and Human Health-Related Risks: An Integrated Assessment Based on Selected Physicochemical Characteristics. Geocarto International , 38, Article ID:2210100. https://doi.org/10.1080/10106049.2023.2210100
Shokoohi, E. and Moyo, N. (2025) Groundwater Quality in a Rural and Urbanized Region in Limpopo Province, South Africa. Environments , 12, Article No. 174. https://doi.org/10.3390/environments12060174
Ndlangamandla, L., Sukdeo, N. and Mukwakungu, S.C. (2024) Water Quality Improvement for Food and Beverage Industry vs. SANS:241. Proceedings of the International Conference on Industrial Engineering and Operations Management , Tokyo, 10-12 September 2024, 1028-1041. https://doi.org/10.46254/ap05.20240242
Florez-Peñaloza, J.R., Mahlknecht, J., Escolero, O., Morales-Casique, E., Montaño-Caro, J.C., Blanco-Gaona, S., et al . (2025) Hydrogeochemical Evolution of a Semiarid Endorheic Basin, with Intense Agricultural and Livestock Activities. Journal of Hydrology , 657, Article ID:133093. https://doi.org/10.1016/j.jhydrol.2025.133093
Hamma, B., Bekkouche, M.F., Bouaicha, F., Elnagdy, K.A., Alzaed, A., Barkat, A., et al . (2025) Hydrogeochemical Assessment of Groundwater for Agricultural Suitability in the Ksour Mountains, Algeria. Scientific Reports , 15, Article No. 36441. https://doi.org/10.1038/s41598-025-20352-8
Sambo, T.D., Gololo, S.S. and Seeletse, S.M. (2025) Borehole Water Quality and Health Risks in Rural Communities: A Consumer Perceptive Analysis. International Journal of Business Ecosystem & Strategy , 7, 420-429. https://doi.org/10.36096/ijbes.v7i3.818
Mosase, E. and Ahiablame, L. (2018) Rainfall and Temperature in the Limpopo River Basin, Southern Africa: Means, Variations, and Trends from 1979 to 2013. Water , 10, Article No. 364. https://doi.org/10.3390/w10040364
World Health Organization (2022) Guidelines for Drinking-Water Quality, Fourth Edition Incorporating the First and Second Addenda. World Health Organization.
Mabe, C.J., Molefe, D.M. and Gololo, S.S. (2024) Investigating the Presence and Levels of Some Selected Chemical Parameters in Borehole Water of Ga-Matlala in Limpopo Province, South Africa: Determining the Potential Risks. Environmental Health Insights , 18, 1-2. https://doi.org/10.1177/11786302241297492
Custodio, M., De la Cruz, H., Huarcaya, J. and Huanay, Y. (2025) Tap Water Quality from Surface and Groundwater Sources in Rural and Urban Communities Evaluated in Contrasting Climatic Seasons and Its Implications for Public Health. Journal of Water and Health , 23, 1196-1214. https://doi.org/10.2166/wh.2025.045
Maluleke, T.P., Dube, S., Sunkari, E.D. and Ambushe, A.A. (2025) Assessment of Borehole Water Quality in Nwadzekudzeku Village, Giyani, Limpopo Province, South Africa: Implication for Potential Human Health Risks. Journal of Trace Elements and Minerals , 11, Article ID: 100206. https://doi.org/10.1016/j.jtemin.2024.100206
Heaviside, C., Witham, C. and Vardoulakis, S. (2021) Potential Health Impacts from Sulphur Dioxide and Sulphate Exposure in the UK Resulting from an Icelandic Effusive Volcanic Eruption. Science of the Total Environment , 774, Article ID: 145549. https://doi.org/10.1016/j.scitotenv.2021.145549
Fuge, R. (2019) Fluorine in the Environment, a Review of Its Sources and Geochemistry. Applied Geochemistry , 100, 393-406. https://doi.org/10.1016/j.apgeochem.2018.12.016
Guissouma, W., Hakami, O., Al-Rajab, A.J. and Tarhouni, J. (2017) Risk Assessment of Fluoride Exposure in Drinking Water of Tunisia. Chemosphere , 177, 102-108. https://doi.org/10.1016/j.chemosphere.2017.03.011
Stadler, S., Talma, A., Tredoux, G. and Wrabel, J. (2012) Identification of Sources and Infiltration Regimes of Nitrate in the Semi-Arid Kalahari: Regional Differences and Implications for Groundwater Management. Water SA , 38, 213-224. https://doi.org/10.4314/wsa.v38i2.6
Fossen Johnson, S. (2019) Methemoglobinemia: Infants at Risk. Current Problems in Pediatric and Adolescent Health Care , 49, 57-67. https://doi.org/10.1016/j.cppeds.2019.03.002
Ma, L., Hu, L., Feng, X. and Wang, S. (2018) Nitrate and Nitrite in Health and Disease. Aging and disease , 9, Article No. 938. https://doi.org/10.14336/ad.2017.1207
Lacalamita, D., Mongioví, C. and Crini, G. (2024) Chemical Oxygen Demand and Biochemical Oxygen Demand Analysis of Discharge Waters from Laundry Industry: Monitoring, Temporal Variability, and Biodegradability. Frontiers in Environmental Science , 12, Article ID:1387041. https://doi.org/10.3389/fenvs.2024.1387041
Adeyemi, A.I. (2020) Bacteriological and Physicochemical Quality of Borehole Water Used for Drinking at Olusegun Agagu University of Science and Technology, Okitipupa, Nigeria. International Journal of Environment , Agriculture and Biotechnology , 5, 890-897. https://doi.org/10.22161/ijeab.54.7
Hama Aziz, K.H., Mustafa, F.S., Omer, K.M., Hama, S., Hamarawf, R.F. and Rahman, K.O. (2023) Heavy Metal Pollution in the Aquatic Environment: Efficient and Low-Cost Removal Approaches to Eliminate Their Toxicity: A Review. RSC Advances , 13, 17595-17610. https://doi.org/10.1039/d3ra00723e
Acosta, J.A., Jansen, B., Kalbitz, K., Faz, A. and Martínez-Martínez, S. (2011) Salinity Increases Mobility of Heavy Metals in Soils. Chemosphere , 85, 1318-1324. https://doi.org/10.1016/j.chemosphere.2011.07.046
Deng, H., Tu, Y., Wang, H., Wang, Z., Li, Y., Chai, L., et al . (2022) Environmental Behavior, Human Health Effect, and Pollution Control of Heavy Metal(loid)s toward Full Life Cycle Processes. Eco - Environment & Health , 1, 229-243. https://doi.org/10.1016/j.eehl.2022.11.003
Liu, W., Qin, D., Yang, Y. and Guo, G. (2023) Enrichment of Manganese at Low Background Level Groundwater Systems: A Study of Groundwater from Quaternary Porous Aquifers in Changping Region, Beijing, China. Water , 15, Article No. 1537. https://doi.org/10.3390/w15081537
DeVore, C.L., Rodriguez-Freire, L., Villa, N., Soleimanifar, M., Gonzalez-Estrella, J., Ali, A.M.S., et al . (2022) Mobilization of As, Fe, and Mn from Contaminated Sediment in Aerobic and Anaerobic Conditions: Chemical or Microbiological Triggers? ACS Earth and Space Chemistry , 6, 1644-1654. https://doi.org/10.1021/acsearthspacechem.1c00370
Odewade, L.O., Imam, A.A., Adesakin, T.A. and Odewade, J.O. (2025) Assessment of Human Faecal Contamination on Groundwater Quality and Reporting Consequent Waterborne Diseases in Funtua Metropolis, Katsina State, Nigeria. Frontiers in Water , 7, Article ID: 1561777. https://doi.org/10.3389/frwa.2025.1561777
Xiao, Q., Wang, B., Li, Z., Zhang, Z., Xie, K., Zhou, J., et al . (2024) The Assembly Process and Co-Occurrence Network of Soil Microbial Community Driven by Cadmium in Volcanic Ecosystem. Resources , Environment and Sustainability , 17, Article ID: 100164. https://doi.org/10.1016/j.resenv.2024.100164
Kristanti, R.A., Hadibarata, T., Syafrudin, M., Yılmaz, M. and Abdullah, S. (2022) Microbiological Contaminants in Drinking Water: Current Status and Challenges. Water , Air , & Soil Pollution , 233, Article No. 299. https://doi.org/10.1007/s11270-022-05698-3
Lin, L., Yang, H. and Xu, X. (2022) Effects of Water Pollution on Human Health and Disease Heterogeneity: A Review. Frontiers in Environmental Science , 10, Article ID: 880246. https://doi.org/10.3389/fenvs.2022.880246
Ben Maamar, S., Aquilina, L., Quaiser, A., Pauwels, H., Michon-Coudouel, S., Vergnaud-Ayraud, V., et al . (2015) Groundwater Isolation Governs Chemistry and Microbial Community Structure along Hydrologic Flowpaths. Frontiers in Microbiology , 6, Article No. 1457. https://doi.org/10.3389/fmicb.2015.01457
Said, I., Abd-Elgawad, A.N., Seleem, E.M., Zeid, S.A.M. and Salman, S.A. (2022) Multivariate Statistics Explaining Groundwater Chemistry, Asyut, Egypt. Environmental Monitoring and Assessment , 194, Article No. 669. https://doi.org/10.1007/s10661-022-10338-8