Culturability and Viability of <i>Salmonella Typhimurium</i> during Photo-Fenton Process at pH 5.5 under Solar Simulated Irradiation — Oak Academic Publishing
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Culturability and Viability of <i>Salmonella Typhimurium</i> during Photo-Fenton Process at pH 5.5 under Solar Simulated Irradiation
Center of Research and Development in Applied Sciences, “Dr. J. J. Ronco” (CINDECA), Chemistry Department, Faculty of Exact Sciences, UNLP-CCT La Plata, Buenos Aires, Argentina
,
Research Group in Oxidation Advanced Processes for Chemical and Biological Treatments (GAOX), University del Valle, Cali, Colombia
,
Research Group in Oxidation Advanced Processes for Chemical and Biological Treatments (GAOX), University del Valle, Cali, Colombia
1 Center of Research and Development in Applied Sciences, “Dr. J. J. Ronco” (CINDECA), Chemistry Department, Faculty of Exact Sciences, UNLP-CCT La Plata, Buenos Aires, Argentina
2 Research Group in Oxidation Advanced Processes for Chemical and Biological Treatments (GAOX), University del Valle, Cali, Colombia
3 Research Group in Oxidation Advanced Processes for Chemical and Biological Treatments (GAOX), University del Valle, Cali, Colombia
Culturability and viability techniques such as plate count on solid agar (PC), Most Probable Number (MPN) and Direct Viable Count-Fluorescence in Situ Hybridation (DVC FISH) were used to study the inactivation of Salmonella typhimurium by photo-Fenton process at pH 5.5. In the presence of only simulated solar irradiation (500 W·m -2) , S. typhimurim showed that both culturability measured by MPN and viability (measured by DVC FISH) underwent just a slight decreasing of 2 and 1 log respectively after 240 min of light exposition while culturability measured by PC did not show any change. Results after 48 h of dark conditions did not reveal re-growth. However, when experiment was carried out in the presence of 2 mg L -1 of Fe 3+ and 20 mg L -1 of H 2 O 2 and pH 5.5, culturability was strongly affected after 240 min of simulated solar irradiation; nevertheless, viability was only slightly altered (~1 log). During dark period of 48 h changes on culturability and viability were not observed. On the other hand, it was also found that sugar metabolism was affected rather the amino-acids in S. typhimurium cells irradiated at different times upon photo-Fenton conditions. These findings might suggest for the first time that photo-Fenton process at pH 5.5 could induce viable but nonculturable state (VBNC) on waterborne S. typhimurium and that probably sugar metabolism damage could activate the VBNC state.
KeywordsPhoto-Fenton DisinfectionSalmonella Typhimurium InactivationVBNC StateViability and Cultivability
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