Generation of Reactive Oxygen Species (ROS) on the Surface of Severe Plastically Deformed Pure Copper Processed by High-Pressure Torsion (HPT) — Oak Academic Publishing
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Generation of Reactive Oxygen Species (ROS) on the Surface of Severe Plastically Deformed Pure Copper Processed by High-Pressure Torsion (HPT)
Research Center of Bio-Micro-Fluidic Science, Doshisha University, Kyoto, Japan
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Department of Chemical Engineering and Materials Science, Faculty of Science & Engineering, Doshisha University, Kyoto, Japan
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Department of Chemical Engineering and Materials Science, Faculty of Science & Engineering, Doshisha University, Kyoto, Japan
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Research Center of Bio-Micro-Fluidic Science, Doshisha University, Kyoto, Japan
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Graduate School of Engineering, Osaka Metropolitan University, Osaka, Japan
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International Institute for Carbon-Neutral Energy Research (I2CNER), Kyushu University, Fukuoka, Japan
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Japan Copper Development Association, Tokyo, Japan
1 Research Center of Bio-Micro-Fluidic Science, Doshisha University, Kyoto, Japan
2 Department of Chemical Engineering and Materials Science, Faculty of Science & Engineering, Doshisha University, Kyoto, Japan
3 Department of Chemical Engineering and Materials Science, Faculty of Science & Engineering, Doshisha University, Kyoto, Japan
4 Research Center of Bio-Micro-Fluidic Science, Doshisha University, Kyoto, Japan
5 Graduate School of Engineering, Osaka Metropolitan University, Osaka, Japan
6 International Institute for Carbon-Neutral Energy Research (I2CNER), Kyushu University, Fukuoka, Japan
7 Japan Copper Development Association, Tokyo, Japan
To investigate the antibacterial activity of severe plastically deformed pure Cu by high-pressure torsion (HPT), the generation of reactive oxygen species (ROS) which might be the main origin of antibacterial activity of Cu, Cu disc (1.00 × 10 − 2 m in diameter, 1.0 × 10 − 3 m thick) has been evaluated using chemiluminescence (CL) with the luminol solution; HPT processes were performed under various torsion turns, 0, 1/4, 1, 3 and 10 turns under uniaxial pressure of 6.0 GPa and rotating speed of 1.667/s (1.0 rpm) at room temperature. Cu disc which was HPT-processed with the 1/4 turn, simply denoted as HPT 1/4 Cu disc, showed the highest summation of CL intensity, ΣCL, among those of other HPTed discs, and this value was 2.7 times higher than that of as-obtained disc. Furthermore, HPT 1/4 disc heated at 523 K (250˚C) in a vacuum (2 × 10 − 3 Pa) for 1.8 × 10 3 s (30 min) brought much higher ΣCL value, about 35 times of as-obtained disc. Then, the components of ROS generated were investigated using the combination of luminol + scavengers corresponding to each ROS. About 45% of ROS were strong antibacterial active hydroxy radical ·OH and hydrogen peroxide H 2 O 2 .
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