Osmium-Polymer Modified Carbon Nanotube Paste Electrode for Detection of Sucrose and Fructose
- 1 Department of Chemistry and Drug Technologies, Sapienza University of Rome, Rome, Italy
- 2 Department of Chemistry, Universidad de Santiago de Chile, Santiago, Chile
- 3 Department of Chemistry and Drug Technologies, Sapienza University of Rome, Rome, Italy
Abstract
The aim of the work was the modification of a carbon nanotube paste electrode with a highly original osmium-polymer hydrogel for the development of a new amperometric biosensor for detection of sucrose and fructose. The biosensor for sucrose is based on the activity of the enzymes invertase and fructose dehydrogenase (FDH) immobilized into a carbon nanotube paste (CNTP) electrode properly modified with the Os-polymer. A second biosensor, for fructose only, i s constructed containing inactive invertase and used for detection of fructose and for signal subtraction. The biosensors exhibit a detection limit for sucrose of 2 m M and for fructose of 1 m M, linearity up to 5 mM for both biosensors, high sensitivity (1.98 m A·cm - 2 ·mM for sucrose and 1.95 m A·cm - 2 ·mM for fructose), a good reproducibility (RSD = 2.5% for sucrose and 2.1% for fructose), fast response time (8 s for sucrose and 4 s for fructose) and a stability of about 4 months for both biosensors when stored under wet conditions at 4° C . Finally, the biosensors were applied for specific determi nation of sucrose and fructose in several commercial fruit juice samples and validated with a commercial spectropho tometric enzymatic kit.
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